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Plasmid#168046PurposeExpresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationGlu 143 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (S87amb)-His
Plasmid#168044PurposeExpresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationSer 87 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (N116amb)-His
Plasmid#168045PurposeExpresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationAsn 116 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (N21amb)-His
Plasmid#168041PurposeExpresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationAsn 21 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-anticalin (I55amb)-His
Plasmid#168042PurposeExpresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).DepositorInsertAnticalin
UseTags6x HistagExpressionBacterialMutationIle 55 was mutated to amber codon (TAG)PromoterT7 promoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK-PatpI (PEP Hypothetical)
Plasmid#123473PurposeMoChlo kitDepositorInsertPatpI (PEP Hypothetical)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-SIRPT1-Loxodonta Africana
Plasmid#75319PurposeGST fusion Loxodonta Africana Sacsin 89-1368DepositorInsertSacsin Loxodonta Africana 89-1368
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
RCAS (A) Radical Fringe (CT#475)
Plasmid#13981DepositorInsertRadical Fringe (RFNG Chicken)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
RCAS (B) Radical Fringe (CT#476)
Plasmid#13982DepositorInsertRadical Fringe (RFNG Chicken)
UseRetroviral; Avian expressionTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
UAS:hGCG-ICAM(1235)- Nrxn1b;cryaa:mCherry
Plasmid#218860Purpose10xUAS promoter driving expression of human glucagon-truncated ICAM-zfNrxn1b fusion protein for use in trans-Tango.DepositorInserthGCG-ICAM(1235)-nrxn1b
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-SauriCas9-KKH-Puro
Plasmid#135966PurposeExpresses SauriCas9-KKH and puromycin resistance genes, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
G384A_pLenti-TetBattP(GT)-BFP-2A-iCasp9-2A-Blast_rtTA3
Plasmid#200630PurposeNegative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GT) landing padDepositorInsertiCasp9
UseLentiviralTagsExpressionMutationPromoterpTRE3GAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR- flag-Lag16-fibcon-iCAM1
Plasmid#205212PurposeThis vector encodes of the synCAM (fibcon - iCAM1) and GFP nanobody LAG16DepositorInsertsynCAM fibcon - iCAM1 and Lag16 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-GFP-iCAM-1-Tether
Plasmid#205187PurposeThis vector encodes of a synCAM (iCAM1 Tether) and GFPDepositorInsertsynCAM iCAM1 tether and GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pColdII-SIRPT1-Xenopus (Silurana) tropicalis
Plasmid#75355PurposeNt His-tag Xenopus (Silurana) tropicalis Sacsin 87-1376DepositorInsertSacsin Xenopus (Silurana) tropicalis 87-1376
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR- flag-Lag16-iCAM-1-Tether
Plasmid#205196PurposeThis vector encodes of the synCAM Tether (iCAM1) and GFP nanobody LAG16DepositorInsertsynCAM iCAM1 Tether and Lag16 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
1203N-CMV-dDiCas7-11-NLS-bGHpolyA
Plasmid#221537PurposeDeactivated DiCas7-11 effector with CMV promoter/enhancer and nuclear localization signal sequence at 5' and 3' end of the coding domainDepositorInsertDiCas7-11
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
1203O-CMV-DiCas7-11-Δ7.1-bGHpolyA
Plasmid#220959PurposeDiCas7-11 effector with Cas7.1 domain deletion mutationDepositorInsertDiCas7-11
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHR- flag-Lag43-iCAM-tether
Plasmid#205211PurposeThis vector encodes of the synCAM tether (iCAM1) and GFP nanobody LAG43DepositorInsertsynCAM iCAM1 tether and Lag43 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Fgβ-anticalin-His-ybbr
Plasmid#168040PurposeExpresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminusDepositorInsertAnticalin
UseTags6x Histag, Fgβ peptide, and ybbr tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only