We narrowed to 705 results for: intein

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pLV302

Plasmid

#119943

Purpose

Expresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cells

Depositor

Gerald Schwank

Publication

Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

Insert

N-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CMV

Available since

May 25, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV N-SpyCas9-Intein, U6-gRNA scaffold (F+E)

Plasmid

#120293

Purpose

AAV Vector for expression of N-terminal SpyCas9 fragment with Intein and a U6-driven F+E gRNA scaffold

Depositor

Dominik Niopek

Publication

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

N-terminal fragment of SpyCas9

Use

AAV and CRISPR

Tags

SV40-NLS and split-intein

Expression

Mammalian

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSiMPls_N + pSiMPls_C

Plasmid

#167203

Purpose

SiMPl plasmid pair (pSiMPls) for use with spectinomycin/streptomycin

Depositor

Barbara Di Ventura

Publication

Palanisamy et al ACS Omega. 2021 May 21;6(22):14148-14153

Inserts

EGFP
SmR 1-75 + gp41-1 N-intein
mRuby3
gp41-1 C-intein + SmR 76-263

Expression

Bacterial

Promoter

AmpR, CAT, pBAD, and pTrc

Available since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ17940-PHR-SFFV-NES*3-PYL1-pHintein_N150-NLS-ABE8e-Cas9-NLS

Plasmid

#252646

Purpose

Human expression vector containing SFFV promoter, ABE8e fused to PYL1 and pH intein

Depositor

Stanley Qi

Publication

Chen et al Nat Cell Biol. 2026 May;28(5):1076-1086.

Insert

NES*3-PYL1-pHintein_N150-NLS-ABE8e-Cas9-NLS

Use

Lentiviral

Promoter

SFFV

Available since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pWP171

Plasmid

#198795

Purpose

Co-expression plasmid for the OLL neurons

Depositor

Paul Sternberg

Publication

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Peat4prom9-NLS-cGAL(DBD)-gp41-1-N-intein

Expression

Worm

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[closed]

Plasmid

#71806

Purpose

Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID

Depositor

Bas van Steensel

Publication

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

FL.hsp70P-Dam[4-HT-intein@L127C]-Myc[closed]

Expression

Insect

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pET41 His-SUMO-Ub4-mEos3.2-Intein-CBD

Plasmid

#259902

Purpose

Express Tetra-ubiquitin-mEos3.2 construct, with N-terminal His-SUMO and C-terminal Intein-CBD tags

Depositor

Hemmo Meyer

Publication

Kracht et al Sci Adv. 2026 Mar 6;12(10):eaea7381. doi

Insert

mEos3.2

Tags

6xHis-SUMO-4xUbiquitin and Intein-Chitin-Binding …

Expression

Bacterial

Promoter

T7

Available since

Sept. 10, 2026

Availability

Academic Institutions and Nonprofits only

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AAV Intein-C-SpyCas9, CMV-AcrIIA4-scaffold (2xBsmBI sites)

Plasmid

#120294

Purpose

AAV Vector for expression of C-terminal SpyCas9 fragemnt with split-intein and a CMV-driven AcrIIA4 (no miR binding sites)

Depositor

Dominik Niopek

Publication

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

C-terminal fragment of SpyCas9

Use

AAV and CRISPR

Tags

split-intein

Expression

Mammalian

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pWP095

Plasmid

#198789

Purpose

Co-expression plasmid for the CEP neurons

Depositor

Paul Sternberg

Publication

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

PF35D11.1-NLS-cGAL(DBD)-gp41-1-N-intein

Expression

Worm

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pWP063

Plasmid

#198799

Purpose

Co-expression plasmid for the PHB neurons

Depositor

Paul Sternberg

Publication

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Posm-10-NLS-cGAL(DBD)-gp41-1-N-intein

Expression

Worm

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP026

Plasmid

#198800

Purpose

Co-expression plasmid for the PHB neurons

Depositor

Paul Sternberg

Publication

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Psrb-6-NLS-gp41-1-C-intein-cGAL(AD)

Expression

Worm

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pWP097

Plasmid

#198790

Purpose

Co-expression plasmid for the CEP neurons

Depositor

Paul Sternberg

Publication

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

PF09E10.7-NLS-gp41-1-C-intein-cGAL(AD)

Expression

Worm

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLV312.3

Plasmid

#119944

Purpose

Expresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cells

Depositor

Gerald Schwank

Publication

Villiger et al Nat Med. 2018 Oct;24(10):1519-1525. doi:

Insert

C-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CMV

Available since

May 25, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]MycLAM

Plasmid

#71807

Purpose

Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID

Depositor

Bas van Steensel

Publication

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

FL.hsp70P-Dam[4-HT-intein@L127C]-Myc-LAM

Expression

Insect

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]MycPC

Plasmid

#71808

Purpose

Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID

Depositor

Bas van Steensel

Publication

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

FL.hsp70P-Dam[4-HT-intein@L127C]-Myc-PC

Expression

Insect

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ14731-PHR-SFFV-NES*3-PYL1-pHintein-NLS-Cas9-NLS

Plasmid

#252644

Purpose

Human expression vector containing SFFV promoter, Cas9 fused to PYL1 and pH intein

Depositor

Stanley Qi

Publication

Chen et al Nat Cell Biol. 2026 May;28(5):1076-1086.

Insert

NES*3-PYL1-pHintein-NLS-Cas9-NLS

Use

Lentiviral

Promoter

SFFV

Available since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pET41 His-SUMO-Ub4-mEos3.2-Tail-Intein-CBD

Plasmid

#259903

Purpose

Express Tetra-ubiquitin-mEos3.2-Tail construct, with N-terminal His-SUMO and C-terminal Intein-CBD tags

Depositor

Hemmo Meyer

Publication

Kracht et al Sci Adv. 2026 Mar 6;12(10):eaea7381. doi

Insert

mEos3.2

Tags

6xHis-SUMO-4xUbiquitin and Intein-Chitin-Binding …

Expression

Bacterial

Promoter

T7

Available since

Sept. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pWP172

Plasmid

#198796

Purpose

Co-expression plasmid for the OLL neurons

Depositor

Paul Sternberg

Publication

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pseb-3-NLS-gp41-1-C-intein-cGAL(AD)

Expression

Worm

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pWP170

Plasmid

#198792

Purpose

Co-expression plasmid for the FLP, PVD neurons

Depositor

Paul Sternberg

Publication

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

PY48G10A.6-NLS-gp41-1-C-intein-cGAL(AD)

Expression

Worm

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pWP101

Plasmid

#198791

Purpose

Co-expression plasmid for the FLP, PVD neurons or the PHC neurons

Depositor

Paul Sternberg

Publication

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pkcnl-4-NLS-cGAL(DBD)-gp41-1-N-intein

Expression

Worm

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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