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M31: pHR-SFFVp- TDP-43C::mCherry::SspB

Plasmid

#122669

Purpose

C terminal domain of TDP43 (218-414) tagged with SspB and mCherry.

Depositor

Clifford Brangwynne

Article

Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

Insert

TDP43 (TARDBP Human)

Use

Lentiviral

Tags

mCherry-SspB

Expression

Mammalian

Mutation

Contains only amino acids 218-414

Promoter

SFFV

Available since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCCLc-MND-B2705-SABR-Backbone

Plasmid

#119051

Purpose

3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

Depositor

David Baltimore

Article

Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCCLc-MND-B2705-KK10-SABR

Plasmid

#119053

Purpose

3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the HIV(gag) KK10 epitope "KRWIILGLNK" cloned via BsmBI

Depositor

David Baltimore

Article

Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

Insert

B2705-KK10-SABR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

MND

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pPKm-248

Plasmid

#90504

Purpose

Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tPCYA - IRES - MTS - tHO1 - P2A - MTS - tFD - P2A - MTS - tFNR

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pPKm-245

Plasmid

#90503

Purpose

Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tHO1 - P2A - MTS - tPCYA - P2A - MTS - tFD - P2A - MTS - tFNR

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pPKm-244

Plasmid

#90502

Purpose

Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tHO1 - P2A - MTS - tPCYA - IRES - MTS - tFD - P2A - MTS - tFNR

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pMT-C34-L

Plasmid

#223967

Purpose

Anti-MT-C34 light chain expression plasmid; to be used with the heavy chain plasmid, pMT-C34-L (Plasmid 223966), to make the antibody.

Depositor

Dmitriy Mazurov

Article

Kalinichenko et al Biology (Basel). 2024 Aug 29;13(9):675.

Insert

Variable domain of mouse anti-MT-C34 IgG1 light chain jointed to constant region of human IgG1 light chain

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFP-MT2A

Plasmid

#11613

Depositor

Steven Johnson

Insert

metallothionein 2A (MT2A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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EGFP-PKCZ

Plasmid

#110512

Purpose

Fluorescent PKCZ

Depositor

Bernardo Mainou

Insert

PKCZ (PRKCZ Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-PKCZ

Plasmid

#110511

Purpose

Fluorescent PKCZ

Depositor

Bernardo Mainou

Insert

PKCZ (PRKCZ Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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PCDHB10-EGFP

Plasmid

#110515

Purpose

Fluorescent PCDHB10

Depositor

Bernardo Mainou

Insert

PCDHB10 (PCDHB10 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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PCDHB10-mCherry

Plasmid

#110516

Purpose

Fluorescent PCDHB10

Depositor

Bernardo Mainou

Insert

PCDHB10 (PCDHB10 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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MT4

Plasmid

#105711

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 4

Depositor

Artur Krężel

Insert

Metallothionein 4 (MT4 Human)

Use

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab13-Q67L

Plasmid

#110502

Purpose

Constitutive Active

Depositor

Bernardo Mainou

Insert

Rab13-Q67L (RAB13 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Q67L

Promoter

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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Yes-EGFP

Plasmid

#110497

Purpose

Fluorescent Yes

Depositor

Bernardo Mainou

Insert

Yes (YES1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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MT1A

Plasmid

#105700

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 1a

Depositor

Artur Krężel

Insert

Metallothionein 1a (MT1A Human)

Use

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-Rab5C-S35N

Plasmid

#110503

Purpose

Dominant Negative

Depositor

Bernardo Mainou

Insert

Rab5C-S35N (RAB5C Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

S35N

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-PKCZdel239

Plasmid

#110514

Purpose

Constitutive Active

Depositor

Bernardo Mainou

Insert

PKCZ (PRKCZ Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Deleted amino terminal 239 amino acids

Promoter

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-PKCZdel239

Plasmid

#110513

Purpose

Constitutive Active

Depositor

Bernardo Mainou

Insert

PKCZ (PRKCZ Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deleted amino terminal 239 amino acids

Promoter

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-IQCF2

Plasmid

#110507

Purpose

Fluorescent IQCF2

Depositor

Bernardo Mainou

Insert

IQCF2 (IQCF2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge