We narrowed to 651 results for: pFAST

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pFastBac1-His6-E3(1)-CUbo-TwinStrep

Plasmid

#212712

Purpose

Expression of His6-E3(1)-CUbo-TwinStrep in insect cells

Depositor

Helle Ulrich

Publication

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Tags

6xHis and CUbo-TwinStrep

Expression

Bacterial

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac(His10-SNAP-Csk)

Plasmid

#177895

Purpose

Baculo expression of His10 tag fused to SNAPtag and human Csk

Depositor

Ron Vale

Publication

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Csk (CSK Human)

Tags

10xHis-TEV-TVMV-SNAP tag

Expression

Insect

Promoter

Polyhedrin

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Clover4-LUMABS-S1 RBD

Plasmid

#183491

Purpose

To express the Clover4-LUMABS-S1 RBD sensor for SARS-CoV-2 antibody dection.

Depositor

Jun Chu, Zongwen Jin

Publication

Deng et al ACS Nano. 2021 Nov 2. doi: 10.1021/acsna

Insert

Clover4-LUMABS-S1 RBD

Expression

Insect

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (787-1899)

Plasmid

#170145

Purpose

Expresses residues 787-1899 of CHD7 in insect cells

Depositor

Robert Kingston

Publication

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

FLAG

Expression

Insect

Mutation

N-terminal truncation of residues 1-786; C-termin…

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (787-2138)

Plasmid

#170146

Purpose

Expresses residues 787-2138 of CHD7 in insect cells

Depositor

Robert Kingston

Publication

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

FLAG

Expression

Insect

Mutation

N-terminal truncation of residues 1-786; C-termin…

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7 (936-2997)

Plasmid

#170147

Purpose

Expresses residues 936-2997 of CHD7 in insect cells

Depositor

Robert Kingston

Publication

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

FLAG

Expression

Insect

Mutation

N-terminal truncation of residues 1-935

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB DPP9S D808N

Plasmid

#169947

Purpose

Purification of catalytically-dead DPP9S from insect cells

Depositor

Hao Wu

Publication

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

HIS-TEV

Expression

Insect

Mutation

D808N

Promoter

polyhedrin

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac-ATG101-ATG13 HORMA

Plasmid

#99332

Purpose

Mammalian expression of one subcomplex from the Human ULK1 complex

Depositor

James Hurley

Publication

Qi et al Structure. 2015 Oct 6;23(10):1848-57. do

Inserts

ATG101 (ATG101 Human)
ATG13 (ATG13 Human)

Tags

GST

Expression

Insect

Promoter

p10 and pH

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(chimpanzee)

Plasmid

#79032

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(chimpanzee)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

polyhedrin

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac-His-Tipin-L195A

Plasmid

#23121

Depositor

Aziz Sancar

Publication

Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

Insert

Tipin (TIPIN Human)

Tags

His

Expression

Insect

Mutation

Changed Leucine 195 to Alanine

Available since

March 3, 2010

Availability

Academic Institutions and Nonprofits only
pFastBac LIC cloning vector with BioBrick polycistronic restriction sites (11A)

Plasmid

#48294

Depositor

Scott Gradia

Publication

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Expression

Insect

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFastBac1-OSF-TRIM5alpha(human)(R332P)

Plasmid

#79037

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(human R332P) (TRIM5 Human)

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Changed Arg 332 to Pro

Promoter

polyhedrin

Available since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac-V2-REPAIR-mini

Plasmid

#252297

Purpose

Expresses REPAIR-mini (V2) in insect cells.

Depositor

Hiroshi Nishimasu

Publication

Ishikawa et al Nat Struct Mol Biol. 2025 Aug;32(8):1567

Insert

REPAIR-mini (V2)

Expression

Insect

Mutation

dPspCas13b H133A/H1058A/Δ929–1090; 30-aa linker; …

Available since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pFastBac SOX Q129H

Plasmid

#192057

Purpose

Expression vector for SOX (ORF37) Q129H

Depositor

Britt Glaunsinger

Publication

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

SOX (ORF37) Q129H

Expression

Insect

Mutation

Q129H

Promoter

Polyhedrin

Available since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-NEDD4L_ΔC2 (157)

Plasmid

#187720

Purpose

Express GST-tagged NEDD4L ΔC2 (157) in insect cells

Depositor

Sandra Gabelli

Publication

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 157-975 (NEDD4L Human)

Tags

GST-HRV 3C site

Expression

Insect

Mutation

deletion of residues 1-156

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBacHTA-NEDD4L_C2-W1-L-W2-HECT

Plasmid

#187723

Purpose

Express His-tagged NEDD4L C2-W1-L-W2-HECT chimera in insect cells

Depositor

Sandra Gabelli

Publication

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 1-418+582-975 (NEDD4L Human)

Tags

His-TEV

Expression

Insect

Mutation

deletion of residues 419-581

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBacHTA-NEDD4L_W1-L-W2-HECT

Plasmid

#187724

Purpose

Express His-tagged NEDD4L W1-L-W2-HECT chimera in inserct cells

Depositor

Sandra Gabelli

Publication

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 153-418+582-975 (NEDD4L Human)

Tags

His-TEV

Expression

Insect

Mutation

deletion of residues 1-152 and 419-581

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBacI-KDAC6 H216A

Plasmid

#224351

Purpose

Expresses human KDAC6 (HDAC6) H216A in insect cells

Depositor

Terry Watt

Publication

Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

Insert

KDAC6 (HDAC6 Human)

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Histidine 216 mutated to alanine

Promoter

Polyhedrin

Available since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBacI-KDAC6 H611A

Plasmid

#224352

Purpose

Expresses human KDAC6 (HDAC6) H611A in insect cells

Depositor

Terry Watt

Publication

Toro et al Biochemistry. 2023 May 2;62(9):1464-1483

Insert

KDAC6 (HDAC6 Human)

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Histidine 611 mutated to alanine

Promoter

Polyhedrin

Available since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBac1-C17orf53-Strep

Plasmid

#211446

Purpose

generation of Bacmid for insect cell expression of C17orf53 C-terminally fused to Strep-Tag

Depositor

Helmut Pospiech

Insert

C17orf53 (HROB Human)

Tags

Strep-Tag

Expression

Insect

Available since

March 1, 2024

Availability

Academic Institutions and Nonprofits only

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