We narrowed to 596 results for: pFAST

1. pFastBac1-OSF-TRIM5alpha(human)(R332P)

   Plasmid

   #79037

   Purpose

   Baculoviral entry vector to produce Bac-to-bac baculoviruses

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OSF-TRIM5alpha(human R332P) (TRIM5 Human)
   

   Tags

   OneSTrEP-FLAG

   Expression

   Insect

   Mutation

   Changed Arg 332 to Pro

   Promoter

   polyhedrin

   Available Since

   March 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac-His-Flag-Tipin-L195A

   Plasmid

   #23120

   Depositor

   Aziz Sancar

   Article

   Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

   Insert

   Tipin (TIPIN Human)
   

   Tags

   Flag- and His

   Expression

   Insect

   Mutation

   Changed Leucine 195 to Alanine

   Available Since

   March 3, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pFastBac-M13-8xHis-ZZ-LTLT-HOOK3

   Plasmid

   #222287

   Purpose

   Expression of HOOK3 in insect cells for purification of untagged adapter (8xHIS-ZZtag can be cleaved off with TEV protease).

   Depositor

   Anne Straube

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Insert

   HOOK3 (HOOK3 Human)
   

   Tags

   8 His and ZZ tag

   Expression

   Insect

   Available Since

   June 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST

   Plasmid

   #223764

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   BNIP3 soluble part (BNIP3 Human)
   

   Tags

   Thrombin cleavage-GST

   Expression

   Insect

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac HTB His-TEV-rDPP9

   Plasmid

   #217326

   Purpose

   Purification of rat DPP9 from insect cells

   Depositor

   Yifan Wang

   Insert

   DPP9
   

   Tags

   His-TEV

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1

   Plasmid

   #216378

   Purpose

   Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)

   Depositor

   William Weis

   Article

   Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

   Insert

   Frizzled-4 (Fzd4 Mouse)
   

   Use

   Baculovirus

   Tags

   HA signal sequence-FLAG

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K

   Plasmid

   #198037

   Purpose

   Used for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Tags

   GST-TEV-EGFP

   Expression

   Insect

   Mutation

   E696K

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac-GST-TEV-mCherry-TBK1

   Plasmid

   #198033

   Purpose

   Used for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Tags

   GST-TEV and mCherry

   Expression

   Insect

   Mutation

   E696K

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac HTB MBP-SARM1 A600G

   Plasmid

   #195764

   Purpose

   Purification of SARM1 from insect cells

   Depositor

   Hao Wu

   Insert

   SARM1 (SARM1 Human)
   

   Tags

   HIS-MBP-TEV

   Expression

   Insect

   Mutation

   A600G

   Promoter

   polyhedrin

   Available Since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac-M13-6His-KIF1C_R169W-GFP

    Plasmid

    #185979

    Purpose

    Baculovirus expression of human KIF1C with R169W mutation and 6xHis and GFP tags.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

    Insert

    KIF1C (KIF1C Human)
    

    Tags

    6xHis and eGFP

    Expression

    Insect

    Mutation

    R169W - mutation causing HSP (SPG58)

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac-M13-6His-KIF1C_P176L-GFP

    Plasmid

    #185978

    Purpose

    Baculovirus expression of human KIF1C with P176L mutation and 6xHis and GFP tags.

    Depositor

    Anne Straube

    Article

    Siddiqui et al Curr Biol. 2022 Sep 12;32(17):3862-3870.

    Insert

    KIF1C (KIF1C Human)
    

    Tags

    6xHis and eGFP

    Expression

    Insect

    Mutation

    P176L - mutation causing HSP (SPG58)

    Available Since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac1-OSF-TRIMCyp (owl monkey)

    Plasmid

    #79035

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5Cyp (owl monkey)
    

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac(His10-Lckg2a,Y394F,Y505F)

    Plasmid

    #177894

    Purpose

    Baculo expression of His10 tag fused to human LckG2A

    Depositor

    Ron Vale

    Article

    Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

    Insert

    Lck (LCK Human)
    

    Tags

    10xHis-TEV

    Expression

    Insect

    Mutation

    G2A,Y394F,Y505F

    Promoter

    Polyhedrin

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac His6 TEV LIC cloning vector (4B)

    Plasmid

    #30115

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6-TEV

    Expression

    Insect

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac Dual PNG-FLAG PLU-HIS

    Plasmid

    #113173

    Purpose

    Baculovirus expression of PNG and PLU

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    PNG, PLU
    

    Use

    Baculovirus

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST

    Plasmid

    #223765

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    BNIP3 soluble part (BNIP3 Human)
    

    Tags

    EGFP-TEV-GST

    Expression

    Insect

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac Dual His6-TEV-CUL2, Rbx1

    Plasmid

    #231000

    Purpose

    Dual expression vector containing mouse CUL2 and Rbx1 for protein puritification from SF9 insect cells

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

    Inserts

    Rbx1 (Rbx1 Mouse)
    CUL2 (Cul2 Mouse)
    

    Tags

    6x HIS

    Expression

    Insect

    Available Since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFastBac HTB His-TEV-rDPP9-FLAG

    Plasmid

    #217328

    Purpose

    Purification of rat DPP9 fusion with C-terminal FLAG tag from insect cells

    Depositor

    Yifan Wang

    Insert

    DPP9
    

    Tags

    FLAG and His-TEV

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBac HTB His-TEV-rDPP9(S729A)

    Plasmid

    #217327

    Purpose

    Purification of catalytically-dead rat DPP9 from insect cells

    Depositor

    Yifan Wang

    Insert

    DPP9
    

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    S729A

    Promoter

    polyhedrin

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFastBac HTB His-TEV-BN-rNLRP1-FLAG

    Plasmid

    #217323

    Purpose

    Purification of Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cells

    Depositor

    Yifan Wang

    Insert

    NLRP1
    

    Tags

    FLAG and His-TEV

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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