We narrowed to 6,573 results for: tac

1. pcDNA3 Mito(OM)-deltaCKAR

   Plasmid

   #222431

   Purpose

   FRET-based reporter for monitoring deltaPKC activity at the mitochondrial outer membrane in cells.

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

   Insert

   Mitochondrial outer membrane delta-selective C kinase activity reporter
   

   Use

   Tags

   CFP and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-Golgi-deltaCKAR

   Plasmid

   #222428

   Purpose

   FRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.

   Depositor

   Alexandra Newton

   Article

   Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

   Insert

   Golgi delta-selective C kinase activity reporter
   

   Use

   Tags

   CFP and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAttB-Tub-HA-WnkDeltaCC

   Plasmid

   #203972

   Purpose

   Fly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression

   Depositor

   Andreas Jenny

   Insert

   Wnk (Wnk Fly)
   

   Use

   Tags

   HA

   Expression

   Insect

   Mutation

   deletion of coiled coil domains 1 and 2

   Promoter

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAttB-Tub-HA-WnkDeltaCC1

   Plasmid

   #203973

   Purpose

   Fly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression

   Depositor

   Andreas Jenny

   Insert

   Wnk (Wnk Fly)
   

   Use

   Tags

   HA

   Expression

   Insect

   Mutation

   deletion of coiled coil domain 1

   Promoter

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAttB-Tub-HA-WnkDeltaCC2

   Plasmid

   #203974

   Purpose

   Fly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression

   Depositor

   Andreas Jenny

   Insert

   Wnk (Wnk Fly)
   

   Use

   Tags

   HA

   Expression

   Insect

   Mutation

   deletion of coiled coil domain 2

   Promoter

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAttB-Tub-HA-WnkDeltaCT

   Plasmid

   #203975

   Purpose

   Fly transformation vector expressing indicated Wnk variant under tubulin promoter for ubiquitous expression

   Depositor

   Andreas Jenny

   Insert

   Wnk (Wnk Fly)
   

   Use

   Tags

   HA

   Expression

   Insect

   Mutation

   C-terminal deletion after RFxV motif

   Promoter

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFsynW SYT1deltaC2AB reporter

   Plasmid

   #197279

   Purpose

   Encodes for viral expression of Synaptotagmin 1 (SYT1) (1 - 140 a.a.) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

   Depositor

   Edwin Chapman

   Article

   Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

   Insert

   SYT1 (1 - 420 a.a.) and HaloTag (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   hSyn (human synapsin I promoter)

   Available since

   July 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PB-TAC-KRAB-dCas9

   Plasmid

   #120355

   Purpose

   piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tet-on and CBh

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SBP-GFP-Tac-TC

   Plasmid

   #162506

   Purpose

   Expresses GFP tagged partial length Tac (TMD and cytosolic tail) in RUSH vector in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

   Insert

   GFP and SBP tagged partial length Tac (TMD and cytosolic tail) (IL2RA Human)
   

   Use

   Tags

   GFP and SBP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GFP-Cstem(7A)-Tac(5A)

    Plasmid

    #162499

    Purpose

    Expresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a with mutations is inserted between GFP and Tac(5A).

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    CD8a stem region with mutations and Tac full length with mutated stem region (IL2RA, CD8A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The five theronine and two serine residues are mu…

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GFP-Cstem-Tac(5A)

    Plasmid

    #162497

    Purpose

    Expresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a is inserted between GFP and Tac(5A).

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    CD8a stem region and Tac full length with mutated stem region (IL2RA, CD8A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The five threonine residues are mutated into alan…

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GFP-Tac-STC(5A)

    Plasmid

    #162491

    Purpose

    Expresses non-O-glycosylated partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    partial length Tac (stem region with mutations, TMD and cytosolic tail) (IL2RA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Tac is in partial length with its stem region, TM…

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GFP-Tac-STC

    Plasmid

    #162490

    Purpose

    Expresses partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    partial length Tac (stem region, TMD and cytosolic tail) (IL2RA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Tac is in partial length with its stem region, TM…

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNOC-stacked-GeNL-Ca

    Plasmid

    #101003

    Purpose

    Construct for fusion expression of GeNL calcium reporter and neomycin/G418 resistance gene by a bidirectional promoter

    Depositor

    Eva Farre

    Article

    Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP-mSPDEF deltaC2

    Plasmid

    #100867

    Purpose

    Mammalian expression of mouse SPDEF truncation

    Depositor

    Noah Shroyer

    Article

    Lo et al Gastroenterology. 2017 Jul;153(1):205-21

    Insert

    Spdef (Spdef Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    truncated protein 1-238aa

    Promoter

    CMV

    Available since

    Sept. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc-GFP-dSUMOdeltaC

    Plasmid

    #85697

    Purpose

    expression of GFP-SUMO1 delta C in Drosophila cells

    Depositor

    Licio Collavin, Giannino Del Sal

    Article

    Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

    Insert

    SUMO (Sumo Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    non-conjugatable SUMO, lacking the C-terminal gly…

    Promoter

    pAc5

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pLEX_970_puro_DEST_V5_TLOC1(220aa)deltaC27

    Plasmid

    #45292

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    TLOC1 (SEC62 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    deleted 27 c-terminal amino acids

    Promoter

    Available since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pRSF-KpDcr1deltaC(GiVL-1)

    Plasmid

    #32032

    Depositor

    David Bartel

    Article

    Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

    Insert

    DCR1(15-355)
    

    Use

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    H123-N143 replaced by P642-Y645 of GiDicer

    Promoter

    T7

    Available since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSF-KpDcr1deltaC(K217A)

    Plasmid

    #32026

    Depositor

    David Bartel

    Article

    Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

    Insert

    DCR1(15-355)
    

    Use

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    K217A

    Promoter

    T7

    Available since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSF-KpDcr1deltaC(N184A)

    Plasmid

    #32025

    Depositor

    David Bartel

    Article

    Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

    Insert

    DCR1(15-355)
    

    Use

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    N184A

    Promoter

    T7

    Available since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge