We narrowed to 2,188 results for: gibson assembly

#120943

MoClo golden gate assembly AB part for PlacO (common LacI-repressed promoter; see DOI: 10.1126/science.1256272). Please see Supplemental Documents for annotated Genbank file.

Richard Murray

Dec. 4, 2019

Academic Institutions and Nonprofits only

#120946

MoClo golden gate assembly AB part for pLbda-CasRQi (strong lambda promoter with downstream dCas binding site for Qi-lab gRNA (UC16m); gRNA sequence taken from DOI: 10.1016/j.cell.2013.02.022).

Richard Murray

Dec. 4, 2019

Academic Institutions and Nonprofits only

#229953

bigMamAct is evolution of biGBac for mammalian cells. Has empty CMV-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

Martin Pelosse

Pelosse et al Synthetic Biology 2025.02.13

Feb. 18, 2025

Academic Institutions and Nonprofits only

#229953

bigMamAct is evolution of biGBac for mammalian cells. Has empty CMV-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

Martin Pelosse

Pelosse et al Synthetic Biology 2025.02.13

Feb. 18, 2025

Academic Institutions and Nonprofits only

#229956

bigMamAct is evolution of biGBac for mammalian cells. Has empty U6-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

Martin Pelosse

Pelosse et al Synthetic Biology 2025.02.13

Feb. 18, 2025

Academic Institutions and Nonprofits only

#229956

bigMamAct is evolution of biGBac for mammalian cells. Has empty U6-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

Martin Pelosse

Pelosse et al Synthetic Biology 2025.02.13

Feb. 18, 2025

Academic Institutions and Nonprofits only

#229954

bigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

Martin Pelosse

Pelosse et al Synthetic Biology 2025.02.13

Feb. 12, 2025

Academic Institutions and Nonprofits only

#229954

bigMamAct is evolution of biGBac for mammalian cells. Has empty EF1alpha-driven cassette; users should clone gene of interest into shuttle plasmid prior to multi-assembly step by Gibson cloning

Martin Pelosse

Pelosse et al Synthetic Biology 2025.02.13

Feb. 12, 2025

Academic Institutions and Nonprofits only

#157788

EcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Michael Rosen

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

March 17, 2022

Academic Institutions and Nonprofits only

#157788

EcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomes

Michael Rosen

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

March 17, 2022

Academic Institutions and Nonprofits only