We narrowed to 2,516 results for: organization Addgene

1. pCR2.1-KRAS-G12V

   Plasmid

   #129091

   Purpose

   KI KRAS G12V mutation into human KRAS locus

   Depositor

   Toshiro Sato

   Article

   Matano et al Nat Med. 2015 Mar;21(3):256-62. doi: 10.

   Insert

   KRAS(G12V) (KRAS Human)
   

   Use

   Homologous recombination donor plasmid

   Tags

   Expression

   Mutation

   G12V

   Promoter

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSS02:cyt-Peredox-mCherry_DS

   Plasmid

   #161747

   Purpose

   cytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plants

   Depositor

   Markus Schwarzländer

   Article

   Steinbeck et al Plant Cell. 2020 Aug 13. pii: tpc.20.002

   Insert

   cyt-Peredox-mCherry_DS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   amino acid substitution D115S in first T-Rex doma…

   Promoter

   Ubiquitin 10

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. S10A mutant of H3S10ph biosensor

   Plasmid

   #120810

   Purpose

   FRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capability

   Depositor

   Yingxiao Wang

   Article

   Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

   Insert

   Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Serine 10 is mutated to Alanine, and Threonine 3,…

   Promoter

   CMV

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-CMV-Vcan-V3-HA

   Plasmid

   #238076

   Purpose

   Expresses Vcan V3 in mice heart tissues

   Depositor

   Yuxuan Guo

   Article

   Bai et al Cell Prolif. 2025 Jun 3:e70070. doi: 10.

   Insert

   Vcan (Vcan Mouse)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-TP53-1

   Plasmid

   #121917

   Purpose

   Encodes sgRNA targeting exon 4 of TP53

   Depositor

   William Hahn

   Article

   Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

   Insert

   TP53 (TP53 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-TP53-2

   Plasmid

   #121918

   Purpose

   Encodes sgRNA targeting exon 9 of TP53

   Depositor

   William Hahn

   Article

   Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

   Insert

   TP53 (TP53 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-R536C-WLS-HA-IRES-GFP

   Plasmid

   #178065

   Purpose

   Expression of WLS-HA protein (R536C mutant)

   Depositor

   Joseph Gleeson

   Article

   Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

   Insert

   WLS (WLS Human)
   

   Use

   Tags

   HA and IRES-GFP

   Expression

   Mammalian

   Mutation

   R536C

   Promoter

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Y478C-WLS-HA-IRES-GFP

   Plasmid

   #178063

   Purpose

   Expression of WLS-HA protein (Y478C mutant)

   Depositor

   Joseph Gleeson

   Article

   Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

   Insert

   WLS (WLS Human)
   

   Use

   Tags

   HA and IRES-GFP

   Expression

   Mammalian

   Mutation

   Y478C

   Promoter

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Y392C-WLS-HA-IRES-GFP

   Plasmid

   #178062

   Purpose

   Expression of WLS-HA protein (Y392C mutant)

   Depositor

   Joseph Gleeson

   Article

   Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

   Insert

   WLS (WLS Human)
   

   Use

   Tags

   HA and IRES-GFP

   Expression

   Mammalian

   Mutation

   Y392C

   Promoter

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-I531T-WLS-HA-IRES-GFP

    Plasmid

    #178064

    Purpose

    Expression of WLS-HA protein (I531T mutant)

    Depositor

    Joseph Gleeson

    Article

    Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

    Insert

    WLS (WLS Human)
    

    Use

    Tags

    HA and IRES-GFP

    Expression

    Mammalian

    Mutation

    I531T

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PGL4.11-COL1A1WT

    Plasmid

    #230915

    Purpose

    Expression of human COL1A1 promoter and testing the promoter activity with luciferase assay.

    Depositor

    Avi Rosenberg

    Article

    Yang et al Lab Invest. 2024 May;104(5):100336. doi:

    Insert

    Human COL1A1 promoter (COL1A1 Human)
    

    Use

    Luciferase

    Tags

    luciferase - Luc2p

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    COL1A1

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMRX-IP-EGFP-PA-BAX(mito)

    Plasmid

    #228942

    Purpose

    Stable expression of EGFP-LOV2(N538E)-BAX-OMP25 in mammalian cells. Photoactivatable BAX localizes on the outer mitochondrial membrane and induces mitochondrial rupture upon blue light stimulation.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    LOV2 domain of phototropin1
    BAX (BAX Human)
    Omp25 (Synj2bp Rat)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N-terminus and C-terminus are deleted. BAX(3-171)…

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMRX-IP-EGFP-PA-BAX(ER)

    Plasmid

    #228941

    Purpose

    Stable expression of EGFP-LOV2(N538E)-BAX-Cyb5a in mammalian cells. Photoactivatable BAX localizes on ER and induces ER rupture upon blue light stimulation.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    LOV2 domain of phototropin1
    BAX (BAX Human)
    Cyb5a (Cyb5a Rat)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N-terminus and C-terminus are deleted. BAX(3-171)…

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMRX-IP-EGFP-PA-BAX(ER, G108V)

    Plasmid

    #233344

    Purpose

    Stable expression of EGFP-LOV2(N538E)-BAX(G108V)-Cyb5a in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    LOV2 domain of phototropin1
    BAX (BAX Human)
    Cyb5a (Cyb5a Rat)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    BAX(3-171), N-terminus and C-terminus are deleted…

    Promoter

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRX-IP-EGFP-PA-BAX(mito, G108V)

    Plasmid

    #233343

    Purpose

    Stable expression of EGFP-LOV2(N538E)-BAX(G108V)-OMP25 in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    LOV2 domain of phototropin1
    BAX (BAX Human)
    Omp25 (Synj2bp Rat)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N-terminus and C-terminus are deleted. BAX(3-171)…

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRX-IP-EGFP-PA-BAX(lyso, G108V)

    Plasmid

    #233345

    Purpose

    Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    LOV2 domain of phototropin1
    BAX (BAX Human)
    TMEM106B (TMEM106B Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    BAX(3-171), N-terminus and C-terminus are deleted…

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRX-IP-EGFP-PA-BAX(lyso)

    Plasmid

    #228939

    Purpose

    Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. Photoactivatable BAX localizes on lysosomal membrane and induces lysosomal rupture upon blue light stimulation.

    Depositor

    Noboru Mizushima

    Article

    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

    Inserts

    LOV2 domain of phototropin1
    BAX (BAX Human)
    TMEM106B (TMEM106B Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N-terminus and C-terminus are deleted. BAX(3-171)…

    Promoter

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDH-EF1a-mCherry-EGFP-LC3B

    Plasmid

    #170446

    Purpose

    Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.

    Depositor

    Sang-Hun Lee

    Article

    Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

    Insert

    mCherry-GFP-LC3 (MAP1LC3B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV x EF1a_EKAREN5-ires-puro

    Plasmid

    #167821

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-CMV-PEmax-IRES-ZeoR

    Plasmid

    #207353

    Purpose

    Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    Prime editor max (PEmax) enzyme
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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