We narrowed to 3,254 results for: actin

1. pET28a-clbH-C-A2-PCP

   Plasmid

   #114157

   Purpose

   Expresses ClbH-C-A2-PCP

   Depositor

   Emily Balskus

   Article

   Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

   Insert

   clbH
   

   Tags

   6xHis-thrombin-T7 tag

   Expression

   Bacterial

   Mutation

   Only contains the C-A2-PCP domains of ClbH

   Available Since

   Aug. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas8

   Plasmid

   #82380

   Purpose

   dCas9 without a promoter in acsA

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   dCas9
   

   Promoter

   none

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas73

   Plasmid

   #82398

   Purpose

   Δccm:Km, used to delete ccm operon

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   ccm deletion
   

   Promoter

   none

   Available Since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLG-deltaA

   Plasmid

   #33151

   Depositor

   Michael Rosbash

   Article

   Legrain et al Cell. 1989 May 19;57(4):573-83.

   Insert

   RP51A-synthetic minimal intron (RPS17A Budding Yeast)
   

   Tags

   LacZ

   Expression

   Yeast

   Mutation

   The RP51A sequence was modified by digestion with…

   Available Since

   Dec. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

5. 413-Met25 Kar2 TorsinA E171Q HDEL

   Plasmid

   #32123

   Depositor

   Susan Lindquist

   Article

   Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

   Insert

   TorsinA (TOR1A Human)
   

   Tags

   HDEL and Kar2

   Expression

   Yeast

   Mutation

   K108A

   Available Since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

6. 413-Met25 Kar2 TorsinA K108A HDEL

   Plasmid

   #32121

   Depositor

   Susan Lindquist

   Article

   Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

   Insert

   TorsinA (TOR1A Human)
   

   Tags

   HDEL and Kar2

   Expression

   Yeast

   Mutation

   K108A

   Available Since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pDS221

   Plasmid

   #34981

   Purpose

   Expresses a high-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   ePDZb1–mCherry
   

   Expression

   Yeast

   Promoter

   TEF

   Available Since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDS220

   Plasmid

   #34980

   Purpose

   Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   ePDZb–mCherry
   

   Expression

   Yeast

   Promoter

   TEF

   Available Since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 mycBioID

   Plasmid

   #35700

   Purpose

   To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag

   Depositor

   Kyle Roux

   Article

   Roux et al J Cell Biol. 2012 Mar 12.

   Has Service

   Cloning Grade DNA

   Insert

   BirA
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   R118G (highly promiscuous form)

   Promoter

   cmv

   Available Since

   April 5, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. TOMM20-PuRRRE

    Plasmid

    #208329

    Purpose

    PuRRRE genetically-encoded tag introduced after TOMM20; to be used with PunyEEER probe peptide.

    Depositor

    Kimberly Beatty

    Article

    Suyama et al Biochemistry. 2023 Jun 6;62(11):1735-174

    Insert

    TOMM20-PuRRRE
    

    Tags

    PuRRRE

    Expression

    Mammalian

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 MCS-BirA(R118G)-HA

    Plasmid

    #36047

    Purpose

    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag

    Depositor

    Kyle Roux

    Article

    Roux et al J Cell Biol. 2012 Mar 12.

    Has Service

    Cloning Grade DNA

    Insert

    BirA(R118G)-HA
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Available Since

    April 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. HIR WT

    Plasmid

    #24049

    Depositor

    Frederick Stanley

    Article

    Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

    Insert

    Insulin Receptor (INSR Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-p150

    Plasmid

    #51412

    Purpose

    expression of full length of chicken DCTN1 p150Glued in mammalian cells

    Depositor

    Trina Schroer

    Article

    Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

    Insert

    DCTN1 (DCTN1 Chicken)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pMXs-puro-EGFP-FAK

    Plasmid

    #38194

    Depositor

    Noboru Mizushima

    Article

    Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

    Insert

    PTK2 protein tyrosine kinase 2 (Ptk2 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pL1P4 ZmUbiP:GRF-GIF:NosT

    Plasmid

    #198048

    Purpose

    MoClo Golden Gate Level 1 Position 4. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

    Depositor

    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing

    Insert

    ZmUbiP::TaGRF4-GIF1::NosT
    

    Use

    Synthetic Biology

    Promoter

    Zea mays (maize) ubiquitin promoter

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pL1P3 ZmUbiP:GRF-GIF:NosT

    Plasmid

    #198047

    Purpose

    MoClo Golden Gate Level 1 Position 3. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

    Depositor

    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing

    Insert

    ZmUbiP::TaGRF4-GIF1::NosT
    

    Use

    Synthetic Biology

    Promoter

    Zea mays (maize) ubiquitin promoter

    Available Since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMP2463

    Plasmid

    #107774

    Purpose

    Expression of EGFP

    Depositor

    Herman Spaink

    Article

    Stuurman et al Mol Plant Microbe Interact. 2000 Nov;13(

    Insert

    pLacZ-EGFP in pBBR1-mcs5
    

    Tags

    pLacZ-EGFP

    Expression

    Bacterial

    Promoter

    pLacZ

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5-mFzd8

    Plasmid

    #42260

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 8 (Fzd8 Mouse)
    

    Expression

    Mammalian

    Available Since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pMXs-IP-EGFP-ULK1

    Plasmid

    #38193

    Depositor

    Noboru Mizushima

    Article

    Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

    Insert

    a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV5 Flag-Smad1

    Plasmid

    #14044

    Depositor

    Joan Massague

    Article

    Liu et al Nature. 1996 Jun 13. 381(6583):620-3.

    Insert

    Smad1 (SMAD1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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