We narrowed to 11,710 results for: pcDNA

1. pcDNA3.1.Gαi2-LgB91

   Plasmid

   #134358

   Purpose

   Nanoluc complementation assay. Expression of Gαi2 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi2. Addition of the HA epitope at N terminus of Gαi2.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   Gαi2-LgB91 (GNAI2 Human)
   

   Use

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   T7

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-CD40L-mgSrtA

   Plasmid

   #125795

   Purpose

   for co-expression of CD40L (CD40 ligand) and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of CD40 positive cells.

   Depositor

   P. Chen

   Article

   Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

   Insert

   CD40L-mgSrtA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pY116 (pcDNA3.1-huMb2Cpf1)

   Plasmid

   #92292

   Purpose

   Expression of humanized Mb2Cpf1

   Depositor

   Feng Zhang

   Insert

   huMb2Cpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA FLAG TORC2

   Plasmid

   #22975

   Depositor

   Marc Montminy

   Article

   Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

   Insert

   TORC2 (CRTC2 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO V5 DNAJB6b

   Plasmid

   #19528

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

   Insert

   DNAJB6b (DNAJB6 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-human LRRC33

   Plasmid

   #111602

   Purpose

   Express human LRRC33 in 293T cells

   Depositor

   Timothy Springer

   Article

   Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

   Insert

   human LRRC33 (NRROS Human)
   

   Use

   Tags

   myc/his tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. LacZ-5BoxB in pcDNA3.1+

   Plasmid

   #29729

   Depositor

   Howard Chang

   Article

   Wang et al Nature. 2011 Mar 20. ():.

   Insert

   LacZ-5BoxB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1/HisA/Serca1

   Plasmid

   #74402

   Purpose

   expression of SERCA in mammalian cells

   Depositor

   Robert Nakamoto

   Insert

   SERCA1 (ATP2A1 rabbit)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   NcoI site destroyed (silent) T898

   Promoter

   CMV

   Available since

   April 6, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1-RON-V5/HIS

   Plasmid

   #201983

   Purpose

   expression of human RON receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   RON receptor tyrosine kinase (MST1R Human)
   

   Use

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-10nm-EBFP2

    Plasmid

    #231184

    Purpose

    Expresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 10 nm

    Depositor

    Dmitry Lim

    Article

    Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

    Insert

    10nm-EBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-sACE2-WT(732)-8h

    Plasmid

    #154101

    Purpose

    Mammalian expression plasmid for dimeric soluble ACE2 (8his tag)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Short GSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease and neck domains …

    Promoter

    CMV

    Available since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5-Flag-Med26

    Plasmid

    #49250

    Purpose

    Mammalian expression of flag-tagged Med26

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    Med 26 (MED26 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1_nV5-AMOT (p130)

    Plasmid

    #172997

    Purpose

    constitutive expression of V5-tagged AMOT (p130)

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    V5-AMOT (p130) (AMOT Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO HSPA8

    Plasmid

    #19460

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    HSPA8 (HSPA8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-P25C-GFP

    Plasmid

    #1343

    Depositor

    Li-Huei Tsai

    Insert

    P25 (CDK5R1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. Human NCKX6 (pcDNA3.1+)

    Plasmid

    #75216

    Purpose

    Mammalian expression of SLC8B1

    Depositor

    Jonathan Lytton

    Article

    Cai et al J Biol Chem. 2004 Feb 13;279(7):5867-76.

    Insert

    SLC8B1 (SLC8B1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-ccdB-3xHA

    Plasmid

    #214986

    Purpose

    Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xHA tag

    Depositor

    Wade Harper

    Type

    Empty backbone

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. FLAG-HA-FbxO5-pcDNA3.1-

    Plasmid

    #52509

    Purpose

    expresses human FbxO5 in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO5 (FBXO5 Human, FbxO5)
    

    Use

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(-) FLIPQTV3.0 100micro

    Plasmid

    #63730

    Purpose

    FLIPQTV3.0 glutamine sensor with D157N mutation

    Depositor

    Sakiko Okumoto

    Article

    Gruenwald et al PLoS One. 2012;7(6):e38591. doi: 10.1371

    Insert

    FLIPQTV3.0 sensor, 100microM affinity
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D157N in glutamine binding domain

    Promoter

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5/FRT/TO DNAJB1

    Plasmid

    #19468

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    DNAJB1 (DNAJB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge