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pGEX-2TK-WW-CR-d-ZZ-W3083F/P3086A

Plasmid

#18079

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Tags

GST

Expression

Bacterial

Mutation

Second conserved Tryptophan (W) of the WW domain …

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pWal10-roe-sfGFP

Plasmid

#240239

Purpose

Gateway destination plasmid to generate C-terminal sfGFP fusions under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Type

Empty backbone

Use

Gateway destination vector

Expression

Insect

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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plenti-ATF4

Plasmid

#125238

Purpose

ATF4 expression vector

Depositor

Rozita Yarmand

Article

Bagheri-Yarmand et al J Biol Chem. 2015 May 1;290(18):11749-61

Insert

ATF4 (ATF4 Human)

Use

Lentiviral

Tags

Flag

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pTUM4

Plasmid

#230905

Purpose

Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli

Depositor

Arne Skerra

Article

Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-

Inserts

FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
SurA (surA E. coli)
DsbA (dsbA E. coli)
DsbC (dsbC E. coli)

Expression

Bacterial

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pGART-GFP

Plasmid

#99106

Purpose

Expresses human GART-GFP fusion in mammalian cells

Depositor

Stephen Benkovic

Article

An et al Science. 2008 Apr 4;320(5872):103-6. doi

Insert

GART (GART Human)

Tags

GFP

Expression

Mammalian

Mutation

A65T, L68V, and A72S mutations in GFP gene; kanR/…

Promoter

CMV

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 TFAM-mScarlet

Plasmid

#129573

Purpose

Expression of mScarlet fused TFAM

Depositor

Stephen Tait

Article

Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

Insert

TFAM (TFAM Human)

Expression

Mammalian

Mutation

None

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRL1 CTF-FLAG

Plasmid

#217706

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRL1 (ADGRL1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-838

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRB3 CTF-FLAG

Plasmid

#217683

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRB3 (BAI3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-856

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pBZCas13b

Plasmid

#89898

Purpose

Bacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

Depositor

Feng Zhang

Article

Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

Insert

Cas13b

Expression

Bacterial

Promoter

Lac

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV_IRES_GFP_CD16a

Plasmid

#196189

Purpose

Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

Depositor

Ana Ruiz-Saenz

Article

Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

Insert

CD16a (FCGR3A Human)

Use

Retroviral

Expression

Mammalian

Mutation

changed Phenylalanine 158 to Valine

Promoter

LTR

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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