We narrowed to 45,330 results for: cha
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Plasmid#101285PurposeLentiviral expression ,was generatedby recombining bothIRES an HA-tagged H-Ras V12 into SbfI site of pLL5.0 Ecadherin shRNA/mEcad-GFPDepositorInsertE-Cadherin-GFP-H-Ras
UseLentiviralTagsGFPExpressionMammalianMutationH-Ras V12Available SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_ZCCHC7_PAX5
Plasmid#205958PurposeExpress mEGFP-tagged fusion protein, ZCCHC7_PAX5 from patient-derived sequenceDepositorAvailable SinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
TPC2(E643A)-mCherry
Plasmid#135187PurposeMutant TPC2 with reduced Ca2+ selectivity, tagged on C terminus with mCherry.DepositorInsertTPC2 (Tpcn2 Mouse)
UseLentiviralTagsmCherryExpressionMammalianMutationMutation of glutamate 643 to alanineAvailable SinceDec. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
CaV1.3e[8a,11,31b,Δ32,Δ42a]
Plasmid#49332PurposeRepaired Addgene #26577 to agree with the rat ref sequence. Repairs are @: nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].DepositorInsertCACNA1D (Cacna1d Rat)
ExpressionMammalianMutationContains exons 8a, 11, and 31b.Exons 32 and 42a a…PromoterCMVAvailable SinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-linker-TlcnC
Plasmid#114377Purposeexpresses Flpo recombinase-dependent GtACR2-Kv2.1C-linker-TlcnC, which targets GtACR2 to the somatodendritic compartment. Messier et al found this hybrid construct was the most effective at targetingDepositorInsertGtACR2-EYFP-Kv2.1C-linker-TlcnC (NEWENTRY )
UseAAVTagsEYFP and Kv2.1C-linker-TlcnCExpressionMammalianPromoterEf1aAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-DEPTOR S286/287/291A
Plasmid#159160PurposeExpresses DEPTOR S286/287/291A mutant in mammalian cellsDepositorInsertDEPTOR (DEPTOR Human)
TagsHAExpressionMammalianMutationchanged serine 286,287 and 291 to alaninePromoterCMVAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-DEPTOR S286/287/291D
Plasmid#159161PurposeExpresses DEPTOR S286/287/291D mutant in mammalian cellsDepositorInsertDEPTOR (DEPTOR Human)
TagsHAExpressionMammalianMutationchanged serine 286,287 and 291 to aspartic acidPromoterCMVAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-FHC-POLQ-DM
Plasmid#64880Purposelentiviral expression of human POLQ double mutant (in POL and HDL domains)DepositorInsertPOLQ (POLQ Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationK121M and D2330A,Y2331APromoterEF1alphaAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-p2A-full length LIC1
Plasmid#74601Purposeexpresses LIC1 in mammalian cells; untagged but cells indicate expression with diffuse mCherryDepositorInsertfull length human dynein light intermediate chain 1 (DYNC1LI1 Human)
UseLentiviralExpressionMammalianPromoterSFFVAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP TEM4 FL
Plasmid#58893PurposeExpresses GFP tagged full length TEM4 protein. Please see notes below for information regarding the L722F mutation in TEM4.DepositorAvailable SinceSept. 15, 2014AvailabilityAcademic Institutions and Nonprofits only