We narrowed to 4,223 results for: Pet2

1. pET28b-NmCas9-Flag

   Plasmid

   #109030

   Purpose

   Wild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

   Depositor

   Yan Zhang

   Article

   Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

   Insert

   CRISPR-associated endonuclease Cas9
   

   Use

   Tags

   Flag Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET24a-ncOGT-TPR

   Plasmid

   #190822

   Purpose

   For expression of the TPR domain of human ncOGT (C467X mutation) in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.

   Depositor

   Gerald Hart

   Article

   Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   C467X. Also codon optimized for expression in E.c…

   Promoter

   T7

   Available since

   Jan. 9, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pET29b-EPN01*-His

   Plasmid

   #84509

   Purpose

   E.coli version of EPN01*

   Depositor

   Neil King

   Article

   Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

   Insert

   EPN01*-His_pET29b
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28 His10-FcRɣ

   Plasmid

   #113021

   Purpose

   for purification of His10-FcRɣ intracellular domain

   Depositor

   Ron Vale

   Article

   Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

   Insert

   His10-FcRɣ intracellular domain
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21-pelB-LaG-17

   Plasmid

   #172747

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-17
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET24a-LaM4-3xTS

   Plasmid

   #162777

   Purpose

   Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to three tyrosine sulfation (TS) motifs. LaM4-3xTS also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   anti-mCherry nanobody fused to three TS sites, T7, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, T7, and TS site from proCCK

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET24b-6His-fSNAP

   Plasmid

   #106999

   Purpose

   To express 6His-fSNAP protein in E.coli

   Depositor

   Melissa Moore

   Article

   Haraszti et al Methods Mol Biol. 2020;2113:17-22. doi:

   Insert

   fSNAP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET24a-VHH-myc

   Plasmid

   #109422

   Purpose

   Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

   Insert

   anti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, T7, and myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28b-Ets1-ETS

   Plasmid

   #85735

   Purpose

   Expresses DNA-binding (ETS) domain of murine Ets-1, residues 311 to 440

   Depositor

   Gregory Poon

   Article

   Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16

   Insert

   DNA-binding (ETS) domain of murine Ets-1, residues 331 to 440 (Ets1 Human, Mouse)
   

   Use

   Tags

   6xHis following thrombin cleavage site LVPR|G

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Brg1 Del2 pET28a

    Plasmid

    #122116

    Purpose

    Expression Brg1 amino acids 1-837 in bacteria

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    Brg1 (SMARCA4 Human)
    

    Use

    Tags

    6XHis and His-T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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