We narrowed to 4,420 results for: DUR

1. pcDNA5 FRT TO GFP MPS1 K-R

   Plasmid

   #59819

   Purpose

   Allows the integration of GFP MPS1 K-R in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Tighe et al J Cell Biol. 2008 Jun 16;181(6):893-901.

   Insert

   Mps1 (TTK Human)
   

   Use

   Tags

   EmGFP

   Expression

   Mammalian

   Mutation

   KD, catalytically inactive, D664A

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-AviMta3-3xFiBsd

   Plasmid

   #140962

   Purpose

   full-length wt Avi-Mta3-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

   Depositor

   Brian Hendrich

   Article

   Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

   Insert

   metastasis associated 3 (Mta3 Mouse, Synthetic)
   

   Use

   Tags

   3xFLAG, Avi, and TEV

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

   Plasmid

   #120564

   Purpose

   Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K

   Promoter

   CMV

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMX-IY LC3A

   Plasmid

   #89290

   Purpose

   For expression of the mammalian Atg8 protein LC3A without a tag

   Depositor

   Michael Lazarou

   Article

   Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

   Insert

   LC3A (MAP1LC3A Human)
   

   Use

   Retroviral

   Tags

   IRES-YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-Ctdnep1_D67E-2xFlag

   Plasmid

   #196519

   Purpose

   Expression of Ctdnep1_D67E-2xFlag

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Ctdnep1_D67E-2xFlag (CTDNEP1 Human)
   

   Use

   Tags

   2xFlag

   Expression

   Mammalian

   Mutation

   D67E mutation (phosphastase dead)

   Promoter

   CMV promoter; T7 promoter

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1+CeGFP-Ctdnep1WTsiR

   Plasmid

   #196517

   Purpose

   Expression of Ctdnep1WT-GFP

   Depositor

   Edgar Gomes

   Article

   Calero-Cuenca et al Curr Biol. 2021 Apr 12;31(7):1521-1530.e

   Insert

   Ctdnep1WTsiR (CTDNEP1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Silent mutations for siRNA resistance

   Promoter

   CMV promoter; T7 promoter

   Available since

   Feb. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLCHKO_TK1_HPRT1_Lb_1

   Plasmid

   #155059

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting TK1 using SpCas9 and HPRT1 using LbCas12a (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA targeting TK1 using SpCas9 and HPRT1 using LbCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/BirA/FLAG-DDX54

   Plasmid

   #97063

   Purpose

   Expresses BirA/FLAG-tagged DDX54 in mammalian cells

   Depositor

   Markus Landthaler

   Article

   Milek et al Genome Res. 2017 Aug;27(8):1344-1359. do

   Insert

   DDX54 (DDX54 Human)
   

   Use

   Tags

   BirA and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   tetO2

   Available since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-SV40P-Tet1 enhancer E

   Plasmid

   #63880

   Purpose

   Luciferase reporter plasmid used to study transcriptional control of murine Tet1

   Depositor

   Kian Peng Koh

   Article

   Sohni et al Mol Cell Biol. 2015 Mar 15;35(6):1026-42

   Insert

   Tet1 enhancer fragment E
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLCHKO_PDPR_exon_deletion_1

    Plasmid

    #155065

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNL2119 pARF7 GG0-GG1

    Plasmid

    #195910

    Purpose

    Level 0 pARF7 promoter with GG0 and GG1 golden gate linkers

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    pARF7 (NPH4 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLCHKO_Ptbp_ex8_3

    Plasmid

    #155083

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLCHKO_SFRS7_exon_deletion_3

    Plasmid

    #155071

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLCHKO_MDM4_exon_deletion_3

    Plasmid

    #155075

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRV-GN

    Plasmid

    #106373

    Purpose

    creating stable line with GFP and G418 resistance

    Depositor

    Tong-Chuan He

    Article

    Liao et al Gene Ther. 2017 Jun;24(6):333-341. doi:

    Insert

    eGFP and geneticin/G418 resistance gene
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMX-IY LC3B

    Plasmid

    #89291

    Purpose

    For expression of the mammalian Atg8 protein LC3B without a tag

    Depositor

    Michael Lazarou

    Article

    Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

    Insert

    LC3B (MAP1LC3B Human)
    

    Use

    Retroviral

    Tags

    IRES-YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. 3021_pETcon-SARS-CoV-2-RBD_K417N_E484K_N501Y

    Plasmid

    #184406

    Purpose

    yeast surface display of the SARS-CoV-2 Beta variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Beta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Use

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    K417N+E484K+N501Y

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. HA-human-BNIP3 delta Exon2

    Plasmid

    #100782

    Purpose

    Mammalian expression of BNIP3 delta Exon2 splice variant

    Depositor

    Joseph Gordon

    Article

    Field et al Cell Death Discov. 2018 Sep 21;4:37. doi

    Insert

    human-BNIP3 delta Exon2 (BNIP3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Exon2 deletion

    Promoter

    CMV

    Available since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. mCherry-FKBP-TPD54(L53P,L67P)

    Plasmid

    #172458

    Purpose

    Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag L53P,L67P mutant

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    TPD54 (TPD52L2 Human)
    

    Use

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Mutation

    changed Leucines 53 and 67 to proline (breaks coi…

    Promoter

    CMV

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMX-IY GBRPL2

    Plasmid

    #89295

    Purpose

    For expression of the mammalian Atg8 protein GABARAPL2 without a tag

    Depositor

    Michael Lazarou

    Article

    Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

    Insert

    GABARAPL2 (GABARAPL2 Human)
    

    Use

    Retroviral

    Tags

    IRES-YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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