We narrowed to 2,516 results for: organization Addgene

1. pPB CAG SMARCA4 T910M-IRES-EGFP

   Plasmid

   #153949

   Purpose

   piggyBac transposon vector with CAG promoter expressing T910M mutant SMARCA4 (FLAG-tagged) and EGFP

   Depositor

   Luca Tiberi

   Article

   Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

   Insert

   SMARCA4 (SMARCA4 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T910M

   Promoter

   CAG

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. miniTol2 x EF1a_EKAREN4 + PGK-puro

   Plasmid

   #167824

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4
   

   Use

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLV x EF1a_EKAREN4(TA)-ires-puro

   Plasmid

   #167830

   Purpose

   Control sensor for EKAREN4

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4(TA)
   

   Use

   Lentiviral

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W, T420A

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. miniTol2 x EF1a_EKAREN4(TA) + PGK-puro

   Plasmid

   #167826

   Purpose

   Control sensor for EKAREN4

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4(TA)
   

   Use

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W, T420A

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. miniTol2 x EF1a_EKAREN4 + PGK-blast

   Plasmid

   #167825

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4
   

   Use

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. P521_VSX2_p2A-h2b_mRuby3

   Plasmid

   #239104

   Purpose

   Used in CRISPR gene editing to Insert a p2A-h2b_mRuby3 sequence before the stop codon of the endogenous human VSX2 gene

   Depositor

   Karl Wahlin

   Article

   Jones et al Cells. 2022 Oct 28;11(21):3412. doi: 10.

   Insert

   VSX2 (VSX2 Human)
   

   Use

   CRISPR

   Tags

   -p2A-h2b-mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous VSX2 promoter

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBIG1a_StrepII-Hook2_FTS_FHIP2A

   Plasmid

   #222302

   Purpose

   Co-expresses full-length human FHF (FTS, StrepII-HOOK2 and FHIP2A) in a pBIG1a vector

   Depositor

   Andrew Carter

   Article

   Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

   Inserts

   HOOK2 (HOOK2 Human)
   FTS (AKTIP Human)
   FHIP2A (FHIP2A Human)
   

   Use

   Tags

   StrepII-Psc

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Lenti-N1303K-ngRNA+14_EF1a-puroR

   Plasmid

   #207359

   Purpose

   Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of N1303K-CFTR via prime editing and EF1a-driven puromycin resistance gene.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   N1303K-CFTR +14 ngRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Lenti-L227R-ngRNA+15_EF1a-puroR

   Plasmid

   #207360

   Purpose

   Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of L227R-CFTR via prime editing and EF1a-driven puromycin resistance gene.

   Depositor

   Marianne Carlon

   Article

   Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

   Insert

   L227R-CFTR +15 ngRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV x EF1a_EKAREN4(TA)-ires-blast

    Plasmid

    #167831

    Purpose

    Control sensor for EKAREN4

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4(TA)
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W, T420A

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLX401-INK4A

    Plasmid

    #121919

    Purpose

    Doxycycline-inducible expression of p16-INK4A product of CDKN2A

    Depositor

    William Hahn

    Article

    Howard et al Cancer Res. 2019 Feb 12. pii: 0008-5472.

    Insert

    p16-INK4A (CDKN2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE Tet ON

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. P532_POU4F2-p2A-tdTomato-p2A-Thy1.2

    Plasmid

    #239102

    Purpose

    Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of POU4F2 (aka BRN3B). This includes the donor casette and a U6 driven guide sequence to be used with Cas9.

    Depositor

    Karl Wahlin

    Article

    Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472

    Insert

    POU4F2 (POU4F2 Human)
    

    Use

    Donor plasmid for integration into the human geno…

    Tags

    p2A-tdTomato-p2A-Thy1.2

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous POU4F2

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Lenti-CMV-MLH1dn-IRES-puroR

    Plasmid

    #207354

    Purpose

    Lentiviral transfer plasmid containing CMV-driven expression cassette encoding MLH1dn for prime editing (PE4 and PE5 systems).

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    MutL homolog 1 (MLH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Δ754-756 dominant negative mutant

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Lenti-N1303K-tevopreQ1-epegRNA+13C>G_EF1a-puroR (PBS 14 - RTT 18)

    Plasmid

    #207356

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a N1303K-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    N1303K-CFTR + 13 C>G tevopreq1-prime editing guide RNA (epegRNA)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PGL4.11-COL1A1mSMAD

    Plasmid

    #230917

    Purpose

    Tested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.

    Depositor

    Avi Rosenberg

    Article

    Yang et al Lab Invest. 2024 May;104(5):100336. doi:

    Insert

    Human COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase-luc2p

    Expression

    Bacterial and Mammalian

    Mutation

    COL1A1 promoter with mutant potential SMAD bindin…

    Promoter

    COL1A1

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lenti-L227R-tevopreQ1-epegRNA+13C>A_EF1a-puroR (PBS 10 - RTT 19)

    Plasmid

    #207357

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a L227R-CFTR correcting prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    L227R-CFTR + 13 C>A tevopreq1-prime editing guide RNA (epegRNA)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. P530_SIX6-p2A-h2b-EGFP

    Plasmid

    #239101

    Purpose

    Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of SIX6. This includes the donor casette and a U6 driven guide sequence to be used with Cas9.

    Depositor

    Karl Wahlin

    Article

    Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472

    Insert

    SIX6 (SIX6 Human)
    

    Use

    Donor plasmid

    Tags

    p2A-h2b-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous SIX6 promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PGL4.11-COL1A1mTEAD

    Plasmid

    #230916

    Purpose

    Comparing with wild type COL1A1 promoter, tested whether mutant TEAD binding sites changed the promoter activity.

    Depositor

    Avi Rosenberg

    Article

    Yang et al Lab Invest. 2024 May;104(5):100336. doi:

    Insert

    Human COL1A1 promoter with mutant TEAD elements (COL1A1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase- luc2p

    Expression

    Bacterial and Mammalian

    Mutation

    mutant potential TEAD bind elements AGGAAT to CTG…

    Promoter

    COL1A1

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBIG1a_StrepII-Hook3_FTS_FHIP1B

    Plasmid

    #222299

    Purpose

    Co-expresses full-length human FHF (FTS, StrepII-HOOK3 and FHIP1B) in a pBIG1a vector

    Depositor

    Andrew Carter

    Article

    Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

    Inserts

    HOOK3 (HOOK3 Human)
    FTS (AKTIP Human)
    FHIP1B (FHIP1B Human)
    

    Use

    Tags

    StrepII-Psc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBIG1a_StrepII-Hook3[451-718]_FTS_FHIP1B

    Plasmid

    #222300

    Purpose

    Co-expresses truncated human FHF (FTS, StrepII-HOOK3[451-718] and FHIP1B) in a pBIG1a vector

    Depositor

    Andrew Carter

    Article

    Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

    Inserts

    HOOK3[451-718] (HOOK3 Human)
    FTS (AKTIP Human)
    FHIP1B (FHIP1B Human)
    

    Use

    Tags

    StrepII-Psc

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge