We narrowed to 22,704 results for: c-MYC

1. pInducer10b-EGFP-KRAS-WT

   Plasmid

   #164935

   Purpose

   Expresses EGFP cDNA and KRAS wild type cDNA in mammalian cells

   Depositor

   Ji Luo

   Article

   Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:

   Inserts

   Enhanced green fluorescent protein
   KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Ubc Promoter and Ubc promoter

   Available since

   May 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pF CAG BFP2 IRES neo

   Plasmid

   #108175

   Purpose

   Expresses a blue fluorescent protein (BFP2) in mammalian cells along with conferring resistance to G418

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   Blue fluorescent protein (BFP2)
   

   Use

   Lentiviral; Fluorescent protein

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCR2.1-KRAS-G12V

   Plasmid

   #129091

   Purpose

   KI KRAS G12V mutation into human KRAS locus

   Depositor

   Toshiro Sato

   Article

   Matano et al Nat Med. 2015 Mar;21(3):256-62. doi: 10.

   Insert

   KRAS(G12V) (KRAS Human)
   

   Use

   Homologous recombination donor plasmid

   Tags

   Expression

   Mutation

   G12V

   Promoter

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1/ZMYM2-FGFR1

   Plasmid

   #167486

   Purpose

   Mammalian expression vector for the ZMYM2-FGFR1 fusion gene associated with myeloid and lymphoid neoplasms

   Depositor

   Nicholas Cross

   Article

   Smedley et al Neoplasia. 1999 Oct;1(4):349-55. doi: 10

   Insert

   ZMYM2-FGFR1 (FGFR1, ZMYM2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pOT_4 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-LTBR

   Plasmid

   #181973

   Purpose

   Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

   Insert

   FMC6.3-BBz CAR; LTBR (LTBR Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pscALPSpuro-SsACE2 (Pig)

   Plasmid

   #158085

   Purpose

   Expresses pig ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   Pig ACE2 (ACE2 Sus scrofa)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-puro/RAF1-P261A

   Plasmid

   #131728

   Purpose

   Mammalian Expression of RAF1

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

   Insert

   RAF1 (RAF1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   P261A

   Promoter

   CMV

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOT_3 - lenti-EFS-FMC6.3-28z-2A-puro-2A-LTBR

   Plasmid

   #181972

   Purpose

   Expresses anti-CD19 CAR (with CD28 signaling domain) linked to puromycin resistance via P2A and to human LTBR via T2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

   Insert

   FMC6.3-28z CAR; LTBR (LTBR Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pscALPSpuro-ClACE2 (Dog)

   Plasmid

   #158083

   Purpose

   Expresses dog ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   Dog ACE2 (ACE2 )
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-GFP-mHP1alpha

    Plasmid

    #181912

    Purpose

    Bacterial protein expression vector for GFP-tagged HP1alpha

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1alpha (Cbx5 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMMK ENTR 1 CMV mAG h-Geminin

    Plasmid

    #206281

    Purpose

    ENTR Vector 1 for MultiSite Gateway assembly. Encodes mAG-hGeminin under the control of CMV

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    mAG-hGeminin (GMNN Human)
    

    Use

    Multimate/gateway entr 1

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pscALPSpuro-RnACE2 (Rat)

    Plasmid

    #158086

    Purpose

    Expresses rat ACE2 in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    Rat ACE2 (Ace2 Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PKAsub-TagRFPT-CAAX

    Plasmid

    #87712

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular super-resolution PKA activity reporter (bimFLINC-AKAR); targeted to plasma membrane; use in conjunction with Dronpa-FHA1 (Addgene #87711).

    Depositor

    Jin Zhang

    Article

    Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

    Insert

    PKAsub-TagRFPT-CAAX
    

    Use

    Tags

    6xHis, C-terminal targeting sequence from Kras, T…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCW57-Cx32D178Y-IRES-GCaMP6s

    Plasmid

    #216796

    Purpose

    Inducible bicistronic lentiviral plasmid for the simultaneous expression of the D178Y mutant form of Connexin 32 and cytosolic GCaMP6s

    Depositor

    Fabio Mammano

    Article

    Tettey-Matey et al Cell Commun Signal. 2024 Dec 5;22(1):589

    Insert

    Cx32D178Y-IRES-GCaMP6s (GJB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D178Y mutant form of Connexin 32

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3_ATP10B-E993A

    Plasmid

    #203698

    Purpose

    expresses ATP10B with the pathogenic mutation E993A

    Depositor

    Peter Vangheluwe

    Article

    Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

    Insert

    ATP10B (ATP10B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E993A

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3_ATP10B-E210A

    Plasmid

    #203697

    Purpose

    expresses ATP10B with the catalytic mutation E210A

    Depositor

    Peter Vangheluwe

    Article

    Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

    Insert

    ATP10B (ATP10B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E210A

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3_ATP10B-D433N

    Plasmid

    #203696

    Purpose

    expresses ATP10B with the catalytic mutation D433N

    Depositor

    Peter Vangheluwe

    Article

    Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

    Insert

    ATP10B (ATP10B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D433N

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3_ATP10B-V748L

    Plasmid

    #203702

    Purpose

    expresses ATP10B with the pathogenic mutation V748L

    Depositor

    Peter Vangheluwe

    Article

    Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

    Insert

    ATP10B (ATP10B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V748L

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3_ATP10B-I1038T

    Plasmid

    #203700

    Purpose

    expresses ATP10B with the pathogenic mutation I1038T

    Depositor

    Peter Vangheluwe

    Article

    Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

    Insert

    ATP10B (ATP10B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    I1038T

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3_ATP10B-R153X

    Plasmid

    #203701

    Purpose

    expresses ATP10B with the pathogenic mutation R153X

    Depositor

    Peter Vangheluwe

    Article

    Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

    Insert

    ATP10B (ATP10B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R153X

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA5-F-Med26-NTD R61A K62A

    Plasmid

    #49256

    Purpose

    Mammalian expression of flag-tagged human Med26-NTD R61A K62A

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    Med 26 (MED26 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    NTD R61A K62A

    Promoter

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA5-F-Med26-NTD- K74A K75A

    Plasmid

    #49252

    Purpose

    Mammalian expression of flag-tagged human Med26-NTD- K74A K75A

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

    Insert

    Med 26 (MED26 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K74A, K75A, aa 1-339

    Promoter

    Available since

    Dec. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pLenti-puro/RAF1-P207S

    Plasmid

    #131726

    Purpose

    Mammalian Expression of RAF1

    Depositor

    Jacques De Grève

    Article

    Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

    Insert

    RAF1 (RAF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    P207S

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMXS-IP Keima-Pex11B

    Plasmid

    #224558

    Purpose

    expresses Keima fused to PEX11B in mammalian cells

    Depositor

    William Kaelin, Gregory Wyant

    Article

    Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

    Insert

    PEX11B (PEX11B Human)
    

    Use

    Retroviral

    Tags

    Keima

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p3E-2xFYVE_hrs

    Plasmid

    #67676

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrs

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    2xFYVEhrs
    

    Use

    Gateway multisite 3' entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MBD_R44Q-IFP2.0C

    Plasmid

    #215749

    Purpose

    Expresses binding-deficient R44Q mutant of MBD1 MBD domain fused to C-terminal part of IFP2.0 as negative control for 5mC detection in BiAD sensors

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    IFP2.0C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDD315 (mTurquoise2^SEC^2xHA)

    Plasmid

    #73343

    Purpose

    mTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Zhang et al J Cell Biol. 2023 Sep 4;222(9):e20230210

    Insert

    mTurquoise2-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    2xHA and C. elegans codon-optimized mTurquoise2

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA5-FRT-TO-eGFP-HF

    Plasmid

    #157735

    Purpose

    mammalian expression of C-terminally His8-Flag tagged eGFP under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    eGFP-HF
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMXs-IP-EGFP-ULK1(K46N)

    Plasmid

    #38197

    Depositor

    Noboru Mizushima

    Article

    Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

    Insert

    a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    changed Lysine 46 to Asparagine

    Promoter

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

30. VAMP2-12aa-mCit

    Plasmid

    #53237

    Purpose

    Fluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Tags

    12aa-linker and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

31. pET28b-His-hCE

    Plasmid

    #112710

    Purpose

    Expresses N-terminally His-tagged human CE/RNGTT in bacterial cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    RNGTT (RNGTT Human)
    

    Use

    Tags

    6xHis and Stop codons placed after RNGTT sequence…

    Expression

    Bacterial

    Mutation

    R594H

    Promoter

    T7

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28-At_PRORP2-His

    Plasmid

    #67870

    Purpose

    bacterial expression of PRORP2 (At2g16650), full coding sequence + C-term. His tag

    Depositor

    Walter Rossmanith

    Article

    Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

    Insert

    PRORP2 (PRORP2 Mustard Weed)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBABE-Src-Rescue

    Plasmid

    #26983

    Depositor

    Joan Massague

    Article

    Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

    Insert

    c-Src (CSK Chicken)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

34. pcDNA3 Flag-MBD1 R44Q-14-mVenusC

    Plasmid

    #191394

    Purpose

    R44Q binding pocket mutant of MBD domain of MBD1 fused to C-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    pcDNA3 Flag-MBD1 R44Q-14-mVenusC
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R44Q

    Promoter

    CMV

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCGN H-Ras 61L

    Plasmid

    #14720

    Depositor

    Adrienne Cox

    Article

    Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

    Insert

    H-ras (HRAS Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    61L

    Promoter

    Available since

    Aug. 29, 2007

    Availability

    Academic Institutions and Nonprofits only

36. Pmyo3::tag:sl1(G16C)

    Plasmid

    #79003

    Purpose

    Expression of exogenous SL1 in C. elegans muscle

    Depositor

    Xiao Liu, Ge Zhan

    Article

    Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

    Insert

    sls-1.2 (sls-1.2 Nematode)
    

    Use

    Tags

    illumina-adapter

    Expression

    Worm

    Mutation

    G16C

    Promoter

    myo-3

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMXs-IP-EGFP-ULK1(1-828)

    Plasmid

    #38198

    Depositor

    Noboru Mizushima

    Article

    Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

    Insert

    a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 829-1051

    Promoter

    Available since

    Sept. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

38. pET28-At_PRORP3-His

    Plasmid

    #67871

    Purpose

    bacterial expression of PRORP3 (At4g21900), full coding sequence + C-term. His tag

    Depositor

    Walter Rossmanith

    Article

    Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

    Insert

    PRORP3 (PRORP3 Mustard Weed)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. KHBD00025

    Plasmid

    #34187

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG3796 (ac Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

40. pMXS-IP Keima-OMP25

    Plasmid

    #224560

    Purpose

    expresses Keima fused to OMP25 mitochondrial localization sequence in mammalian cells

    Depositor

    William Kaelin, Gregory Wyant

    Article

    Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

    Insert

    Keima-OMP25 (SYNJ2BP Human)
    

    Use

    Retroviral

    Tags

    Keima

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMXS-IP DEPP1-mCherry

    Plasmid

    #224559

    Purpose

    Expresses DEPP1 fused to mCherry in mammalian cells

    Depositor

    William Kaelin, Gregory Wyant

    Article

    Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

    Insert

    DEPP1 (DEPP1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pMXS-IP DEPP1 dTSnare-mCherry

    Plasmid

    #224561

    Purpose

    Expresses DEPP1 dTsnare fused to mCherry in mammalian cells

    Depositor

    William Kaelin, Gregory Wyant

    Article

    Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

    Insert

    DEPP1 (DEPP1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    removed Tsnare domain

    Promoter

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pETM33_YES1_SH3

    Plasmid

    #178492

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    YES1_SH3 (YES1 Synthetic, Human)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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44. JW777

    Plasmid

    #64818

    Purpose

    BiFC CUC3

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

    Insert

    CUC3 (CUC3 Mustard Weed)
    

    Use

    Tags

    C-terminal domain Firefly

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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45. pIR197

    Plasmid

    #64819

    Purpose

    BiFC rCUC2

    Depositor

    Detlef Weigel

    Article

    Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

    Insert

    CUC2 (CUC2 Mustard Weed)
    

    Use

    Tags

    C-terminal domain Firefly

    Expression

    Mutation

    rCUC2

    Promoter

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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46. mCherry-APPP1-mGFP

    Plasmid

    #196705

    Purpose

    As mCherry-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    mCherry and mEGFP

    Expression

    Mammalian

    Mutation

    Lys to Val substitution at position 612

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. pDNA2.0 6H-TEV-KRAS G12V

    Plasmid

    #111850

    Purpose

    Expresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.

    Depositor

    Kenneth Westover

    Article

    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.

    Insert

    KRAS G12V (KRAS Human)
    

    Use

    Tags

    6xHis-tag and TEV protease cleavage sequence

    Expression

    Bacterial

    Mutation

    Changed Glycine 12 to Valine

    Promoter

    T5

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. mBFP-APPP1-mGFP

    Plasmid

    #196695

    Purpose

    As mBFP-APP-mGFP, but with substitution at position 612 that prevents alpha-secretase activity

    Depositor

    Martino Calamai

    Article

    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

    Insert

    Amyloid Precursor Protein 695 isoform (APP Human)
    

    Use

    Tags

    mEGFP and mtagBFP2

    Expression

    Mammalian

    Mutation

    Lys to Val substitution at position 612

    Promoter

    CMV

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pET28b-SpRY-His

    Plasmid

    #179320

    Purpose

    Plasmid for bacterial expression and purification of SpRY-Cas9

    Depositor

    Miguel Angel Moreno-Mateos, Julián Cerón

    Article

    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

    Insert

    Human codon-optimized Cas9 variant SpRY
    

    Use

    CRISPR

    Tags

    6xHis and SV40-NLS

    Expression

    Bacterial

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pInducer10b-EGFP-KRAS G12V

    Plasmid

    #164925

    Purpose

    Expresses EGFP cDNA and KRAS G12V cDNA in mammalian cells

    Depositor

    Ji Luo

    Article

    Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:

    Inserts

    Enhanced green fluorescent protein
    KRAS proto-oncogene, GTPase (KRAS) G12V mutant (KRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Glycine 12 changed to valine (G->T 3156)

    Promoter

    Ubc Promoter and Ubc promoter

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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