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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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Showing: 2221 - 2240 of 21628 results

  4. pCDM4-GLY

    Plasmid
    #49808
    Purpose
    Encodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration.
    Depositor
    Mattheos Koffas
    Article
    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc
    Inserts
    phosphoglycerate kinase
    glyceraldehyde-3-phosphate dehydrogenase
    pyruvate dehydrogenase subunit E1
    dihydrolipoamide acetyltransferase
    dihydrolipoamide dehydrogenase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    49808-plasmid-map-sequence-id-74623

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  9. pGBW-m4046795

    Plasmid
    #145598
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145598-plasmid-map-sequence-id-297835

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  12. pGBW-m4046740

    Plasmid
    #145588
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 endoRNAse
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145588-plasmid-map-sequence-id-292572

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  13. pVCERC-5 Pvan-dcas9 (Sth3)

    Plasmid
    #133319
    Purpose
    expresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    dcas9 (Streptococcus thermophilus #3)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    vanillate
    Available since
    Jan. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133319-plasmid-map-sequence-id-266830

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  15. pREF-GFP kan

    Plasmid
    #122061
    Purpose
    reporter plasmid for termination and terminator override to express GFP. Marked with URA3
    Depositor
    Daniel Reines
    Article
    Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)
    Inserts
    intergenic terminator from yeast IMD2
    green fluorescent protein
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL1 comes in pYES2
    Available since
    Feb. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    122061-plasmid-map-sequence-id-236157

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  16. pGBW-m4046747

    Plasmid
    #149249
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149249-plasmid-map-sequence-id-358332

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  17. pGBW-m4046733

    Plasmid
    #145665
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 27, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145665-plasmid-map-sequence-id-292478

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  20. pGBW-m4046916

    Plasmid
    #145627
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp6
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145627-plasmid-map-sequence-id-292495

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Showing: 2221 - 2240 of 21628 results