We narrowed to 7,531 results for: ski

1. pGEX-6P-1 Stu2 1-590

   Plasmid

   #38314

   Depositor

   Anthony Hyman

   Article

   Widlund et al Mol Biol Cell. 2012 Sep 19.

   Insert

   5' 1770 bp from STU2 ORF (STU2 Budding Yeast)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP

   Plasmid

   #175521

   Purpose

   Nuclear expression of HaloTag10 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag10
   

   Expression

   Mammalian

   Mutation

   HaloTag10 = HaloTag7-Q165W

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGDP1 fosA

   Plasmid

   #112889

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   fosA
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   Pbla

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMJ806

   Plasmid

   #39312

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. NLS-Halo

   Plasmid

   #200197

   Purpose

   Expression for SV40 NLS

   Depositor

   Sheila Teves

   Article

   Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

   Insert

   3X SV40 NLS-Halo
   

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   None

   Promoter

   EF_1alpha

   Available Since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBabe-tetoff-mVenus-PICdc42

   Plasmid

   #91876

   Purpose

   PI(WT): Photo inhibitable (blue light sensitive)

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Insert

   Vav2 (VAV2 Human)
   

   Tags

   mVenus

   Expression

   Bacterial and Mammalian

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT2SC

   Plasmid

   #59382

   Purpose

   Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.

   Depositor

   Shelley Copley

   Article

   Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

   Insert

   TT-ISceI-cat
   

   Use

   Template

   Available Since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP

   Plasmid

   #175522

   Purpose

   Nuclear expression of HaloTag11 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag11
   

   Expression

   Mammalian

   Mutation

   HaloTag11 = HaloTag7-M175W

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO_IgKchL_HA_HaloTag9_myc_PDGFRtmb

   Plasmid

   #175544

   Purpose

   Expression of HaloTag9 at the outer leaflet of the plasma membrane of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   IgKchL_HA-HaloTag9-myc_PDGFRtmb
   

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTriEx-NTOM20-mVenus-Zdk1

    Plasmid

    #81010

    Purpose

    Expression of Zdk1 anchored to mitochondria

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk1
    

    Tags

    6x His tag, FLAG, NTOM20, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. mito-mScarlet

    Plasmid

    #141153

    Purpose

    Fluorescent labeling of the mitochondrial matrix in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    COX4 (COX4 Budding Yeast)
    

    Tags

    mScarlet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYZ038

    Plasmid

    #200227

    Purpose

    universal template to build tRNA-gRNA array for multiplex editing CREEPY

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    tRNA-gRNA scaffold
    

    Use

    Synthetic Biology

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDCC6

    Plasmid

    #59985

    Purpose

    Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassette

    Depositor

    Peter Duchek

    Article

    Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

    Inserts

    Cas9
    U6-2>sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG

    Promoter

    U6-96Ab and hsp70Bb

    Available Since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-luxNeon

    Plasmid

    #229947

    Purpose

    Mammalian expression of a de novo designed, FRET-based neoLux1.2-mNeonGreen fusion bioluminescent probe (max. Em=518nm)

    Depositor

    Andy Yeh

    Article

    Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

    Insert

    luxNeon
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. Myc-PSF-WT

    Plasmid

    #35183

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

    Insert

    PSF (SFPQ Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGFP-Rab8A[Q67L]

    Plasmid

    #24900

    Depositor

    Maxence Nachury

    Article

    Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

    Insert

    hRab8A[Q67L]
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Glutamine 67 to Leucine

    Available Since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGFP-Rab8A[T22N]

    Plasmid

    #24899

    Depositor

    Maxence Nachury

    Article

    Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

    Insert

    hRab8A[T22N]
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Threonine 22 to Asparagine

    Available Since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGDP2 MCR-1

    Plasmid

    #118404

    Purpose

    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the pLac promoter.

    Depositor

    Gerard Wright

    Article

    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

    Insert

    MCR-1
    

    Expression

    Bacterial

    Promoter

    pLac

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLIK-rABE-Flag

    Plasmid

    #191383

    Purpose

    Doxycycline inducible expression of RNA A-to-I editor rABE

    Depositor

    Stephen Floor

    Article

    Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

    Insert

    rABE-Flag
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    tet-inducible promoter

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. E.coli RaPID plasmid

    Plasmid

    #107251

    Purpose

    Expresses the E.coli RaPID labeling protein

    Depositor

    Paul Khavari

    Article

    Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

    Insert

    E.coli BirA*
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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