We narrowed to 10,632 results for: t7

1. pJK691

   Plasmid

   #72381

   Purpose

   Produces Escherichia coli BolA, N-terminal His6 tag (H6EcBolA)

   Depositor

   T. J. Kappock

   Insert

   stationary-phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC (bolA Escherichia coli str. K-12 substr. MG1655)
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK292

   Plasmid

   #73371

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asn/His89>Asn mutant (AaPurE1-H59N,H89N)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   changed histidine-59 to asparagine and histidine-…

   Promoter

   T7

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJK293

   Plasmid

   #73372

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Phe/His89>Phe mutant (AaPurE1-H59F,H89F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   changed histidine-59 to phenylalanine and histidi…

   Promoter

   T7

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJK294

   Plasmid

   #73373

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, His59>Asp/His89>Asp mutant (AaPurE1-H59D,H89D)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   changed histidine-59 to aspartate and histidine-8…

   Promoter

   T7

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK439

   Plasmid

   #72478

   Purpose

   Produces Thermoplasma acidophilum citrate synthase pseudo-dimer

   Depositor

   T. J. Kappock

   Insert

   citrate synthase (artificial dimer)
   

   Expression

   Bacterial

   Mutation

   inserted NSTCLVPRGSPV sequence between C- and N-t…

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK404

   Plasmid

   #72417

   Purpose

   Produces Acetobacter aceti 1023 Est12, N-terminal His6 tag

   Depositor

   T. J. Kappock

   Insert

   acyl-CoA thioesterase
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   additional N-terminal 15 residues MSETYGTGQKEQHC,…

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK268

   Plasmid

   #72436

   Purpose

   Produces Acetobacter aceti 1023 del7-GloA

   Depositor

   T. J. Kappock

   Insert

   lactoylglutathione lyase
   

   Expression

   Bacterial

   Mutation

   Missing residues 1-7

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK627

   Plasmid

   #72462

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asp/Tyr154>Phe mutant (AaPurE1-S57D,Y154F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   Mutation changes serine-57 to aspartate, tyrosine…

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJK628

   Plasmid

   #72463

   Purpose

   Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Ala/Tyr154>Phe mutant (AaPurE1-S57A,Y154F)

   Depositor

   T. J. Kappock

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Expression

   Bacterial

   Mutation

   Mutation changes serine-57 to alanine, tyrosine-1…

   Promoter

   T7

   Available Since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJK573

    Plasmid

    #72456

    Purpose

    Produces Yersinia pestis N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal TEV protease-cleavable His6 tag (H6tYpPurE1), synthetic gene

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Mutation

    synthetic gene with ~57 silent substitutions

    Promoter

    T7

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJK632

    Plasmid

    #72465

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Val/Tyr154>Phe mutant (AaPurE1-S57V,Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    Mutation changes serine-57 to valine, tyrosine-15…

    Promoter

    T7

    Available Since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJK629

    Plasmid

    #72464

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Thr/Tyr154>Phe mutant (AaPurE1-S57T,Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    Mutation changes serine-57 to threonine, tyrosine…

    Promoter

    T7

    Available Since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK640

    Plasmid

    #72467

    Purpose

    Encodes Escherichia coli evgA

    Depositor

    T. J. Kappock

    Insert

    response regulator in two-component regulatory system with EvgS (evgA Escherichia coli str. K-12 substr. MG1655)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJK541

    Plasmid

    #72447

    Purpose

    Produces Acetobacter aceti 1023 small hypothetical protein (AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)

    Depositor

    T. J. Kappock

    Inserts

    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Expression

    Bacterial

    Promoter

    T7 and n/a

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJK543

    Plasmid

    #72516

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS); large subunit (AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Expression

    Bacterial

    Mutation

    contains additional Ala2

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJK587

    Plasmid

    #71696

    Purpose

    Produces Acetobacter aceti 1023 thioredoxin reductase 1 with a C-terminal His6 tag and Cys138>Ser mutant (AaTrxB1H6-C138S)

    Depositor

    T. J. Kappock

    Insert

    thioredoxin reductase 1
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes cysteine-138 to serine

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK601

    Plasmid

    #71606

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Tyr154>Phe mutant (AaPurE1-Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Expression

    Bacterial

    Mutation

    changes tyrosine-154 to phenylalanine, additional…

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 4Z31

    Plasmid

    #69958

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RC3H2:YTC020-E10:C234061 (RC3H2 Human)
    

    Tags

    His and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO

    Available Since

    Oct. 7, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. 4Z0O

    Plasmid

    #69954

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ZCWPW2:JMC01B-H12:C203050 (ZCWPW2 Human)
    

    Tags

    His and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO

    Available Since

    Oct. 7, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. 4QQM

    Plasmid

    #61950

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RFXANK:JMC029-A03:C213522 (RFXANK Human)
    

    Tags

    6xHis and MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits