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pPyCAG-HA-EGFP-Myc-TM-IRES-Pac

Plasmid

#183605

Purpose

Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Use

Tags

HA, Human PDGFRB transmembrane domain, Mouse IgGK…

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-mouse Gfi1-IRES-GFP

Plasmid

#91891

Purpose

Retroviral expression of mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Gfi1 (Gfi1 Mouse)

Use

Retroviral

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Promoter

MMLV gag

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-H2B-mRuby3-IRES-eGtACR1-ST

Plasmid

#108960

Purpose

Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

eGtACR1-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast 3XFLAG-CHP1

Plasmid

#173166

Purpose

Retroviral vector to express sgRNA resistant 3XFLAG tagged human CHP1

Depositor

Kivanc Birsoy

Article

Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

Insert

Calcineurin Like EF-Hand Protein 1 (CHP1 Human)

Use

Retroviral

Tags

3XFLAG

Expression

Mutation

Synonymous mutations at sgRNA sites

Promoter

MMLV

Available since

Aug. 27, 2021

Availability

Academic Institutions and Nonprofits only

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Cabp5 (445bp crit)-tdTomato-IRES-AP

Plasmid

#18812

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

Cabp5 (445bp crit)-tdTomato-IRES-AP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only
pLVX-IRES-hygro-EGFP-Stx17

Plasmid

#174652

Purpose

Vector for overexpression of EGFP-tagged Stx17 with IRES for hygromycin selection.

Depositor

Lukas Kapitein

Article

Janssen et al J Cell Sci. 2021 Oct 1;134(19). pii: 271

Insert

EGFP-Stx17 (STX17 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

CMV

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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CAG::PSAML141F,Y115F:GlyR-IRES-GFP

Plasmid

#32480

Purpose

Expresses PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAML141F,Y115F-GlyR

Use

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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SV40 basal promoter-tdTomato-IRES-AP

Plasmid

#18809

Depositor

Connie Cepko

Article

Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

Insert

SV40 basal promoter-tdTomato-IRES-AP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only
CaM34(D93A/D129A)/pIRES2-eGFP

Plasmid

#111517

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

calmodulin [Xenopus laevis] (calm2 Frog)

Use

Tags

Expression

Mammalian

Mutation

D93A/D129A

Promoter

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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hSyn-GW-dCas9-SET2-IRES2-mCherry

Plasmid

#202089

Purpose

Neuron-specific expression vector of dCas9-Set2

Depositor

Elizabeth Heller

Article

Xu et al Neuron. 2021 Sep 15;109(18):2943-2966.e8

Insert

dCas9-Set2

Use

Tags

3x FLAG and mCherry

Expression

Mammalian

Mutation

Promoter

human Synapsin

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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