We narrowed to 162,408 results for: addgene

1. NBS1 C-terminal sgRNA

   Plasmid

   #207092

   Purpose

   pX330 based plasmid for expression of Cas9 and the TAAAAAGGAGAAGATAACTG sgRNA to target the NBS1 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   TAAAAAGGAGAAGATAACTG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. RNF169 C-terminal sgRNA

   Plasmid

   #207099

   Purpose

   pX330 based plasmid for expression of Cas9 and the ACACTTCATTAGGTGCTACT sgRNA to target the RNF169 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   ACACTTCATTAGGTGCTACT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. SHLD1 N-terminal sgRNA

   Plasmid

   #207101

   Purpose

   pX330 based plasmid for expression of Cas9 and the ATGGCAGGACTATGGCAGCC sgRNA to target the SHLD1 locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   ATGGCAGGACTATGGCAGCC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. ATM C-terminal sgRNA

   Plasmid

   #207097

   Purpose

   pX330 based plasmid for expression of Cas9 and the TTTCTAAAGGCTGAATGAAA sgRNA to target the ATM locus.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   TTTCTAAAGGCTGAATGAAA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. GOLIM4 g2 LentiCRISPRv2-mCherry

   Plasmid

   #218663

   Purpose

   Knockout vector for human GOLIM4 (GPP130/GOLPH4)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   GOLIM4 (GOLIM4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. GOLIM4 g1 LentiCRISPRv2-mCherry

   Plasmid

   #218662

   Purpose

   Knockout vector for human GOLIM4 (GPP130/GOLPH4)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   GOLIM4 (GOLIM4 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. SHLD3 KO sgRNA #1

   Plasmid

   #207105

   Purpose

   pX330 based plasmid for expression of Cas9 and the CGCTATCAAGATTTATACCT sgRNA to target the SHLD3 locus..

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   CGCTATCAAGATTTATACCT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ATG13 sgRNA

   Plasmid

   #207558

   Purpose

   pX330 expressing Cas9 and a sgRNA targeting the ATG13 locus

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   ggaaactgatctcaattccc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV/CMV-SCLM

   Plasmid

   #216758

   Purpose

   AAV2 transfer vector with the CMV promoter for ubiquitous expression of SuperClomeleon

   Depositor

   George Augustine

   Insert

   SuperClomeleon followed by WPRE and human growth hormone polyA terminator
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   S30R and 11 AA deletion in the Cerulean CFP moiet…

   Promoter

   CMV (cytomegalovirus)

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. SHLD2 N-terminal sgRNA

    Plasmid

    #207098

    Purpose

    pX330 based plasmid for expression of Cas9 and the TTTTTATCAGAAATCATGAG sgRNA to target the SHLD2 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    TTTTTATCAGAAATCATGAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. HaloTag KO sgRNA

    Plasmid

    #207102

    Purpose

    pX330 based plasmid for expression of Cas9 and the GTCGATGTTGGTCCGCGCGA sgRNA to target the HaloTag coding sequence.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GTCGATGTTGGTCCGCGCGA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. MDC1 N-terminal sgRNA

    Plasmid

    #207090

    Purpose

    pX330 based plasmid for expression of Cas9 and the GTATCCTTCCCAGATCATGG sgRNA to target the MDC1 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GTATCCTTCCCAGATCATGG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SHLD3 KO sgRNA #2

    Plasmid

    #207106

    Purpose

    pX330 based plasmid for expression of Cas9 and the CTGAAGGAACAGACTAATTC sgRNA to target the SHLD3 locus..

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    CTGAAGGAACAGACTAATTC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 53BP1 KO sgRNA

    Plasmid

    #207104

    Purpose

    pX330 based plasmid for expression of Cas9 and the AGATTCTCAGCCTGAAAGCC sgRNA to target the 53BP1 coding sequence.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    AGATTCTCAGCCTGAAAGCC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLIK NPIPB11-FLAG zeo

    Plasmid

    #192331

    Purpose

    Lentiviral expression vector for an inducible NPIPB11-FLAG

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    NPIPB11 (NPIPB11 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEN_TT NPIPB11-FLAG

    Plasmid

    #192308

    Purpose

    Gateway entry vector for an inducible NPIPB11-FLAG

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    NPIPB11 (NPIPB11 Human)
    

    Use

    Gateway entry vector

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEN_TT 3xFLAG-NUP37

    Plasmid

    #192299

    Purpose

    Gateway entry vector for an inducible 3xFLAG-NUP37

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    NUP37 (NUP37 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEN_TT 3xFLAG-PFN2_variant2

    Plasmid

    #192300

    Purpose

    Gateway entry vector for an inducible 3xFLAG-PFN2_variant2

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    PFN2_variant2 (PFN2 Human)
    

    Use

    Gateway entry vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pL1P6 ZmUbiP:GRF-GIF:NosT

    Plasmid

    #198049

    Purpose

    MoClo Golden Gate Level 1 Position 6. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

    Depositor

    John Innes Centre - Wheat Transformation and Genome Editing, Cristobal Uauy

    Insert

    ZmUbiP::TaGRF4-GIF1::NosT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Zea mays (maize) ubiquitin promoter

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Venus-Bik-cb5-pEGFP-C1

    Plasmid

    #177431

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) replaced with Cb5 targeting sequence. Swap made in original Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Bik MBR removed (after position 136) and replaced…

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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