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pCMV(MinDis)-iCytSnFR_PM

Plasmid

#177743

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pCMV(MinDis)-iCytSnFR_PM

Expression

Mammalian

Promoter

hCMV

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn iCyt_BrEt_SnFR_ER

Plasmid

#177756

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn iCyt_BrEt_SnFR_ER

Use

AAV

Promoter

hSyn

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iCyt_F_SnFR_ER

Plasmid

#177754

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pAAV9-hSyn-iCyt_F_SnFR_ER

Use

AAV

Promoter

hSyn

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iCyt_BrEt_SnFR_ER

Plasmid

#177746

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pCMV(MinDis)-iCyt_BrEt_SnFR_ER

Expression

Mammalian

Promoter

hCMV

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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_pCMV(MinDis)-iCyt_F_SnFR_ER

Plasmid

#177744

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

_pCMV(MinDis)-iCyt_F_SnFR_ER

Expression

Mammalian

Promoter

hCMV

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iCytSnFR_ER

Plasmid

#177742

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

Insert

pCMV(MinDis)-iCytSnFR_ER

Expression

Mammalian

Promoter

hCMV

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFP-LC3-RFP-LC3ΔG

Plasmid

#168997

Purpose

Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

Depositor

Noboru Mizushima

Article

Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

EGFP and mRFP1

Expression

Mammalian

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1

Plasmid

#83842

Purpose

Fluorescent probe for S/G2 cell cycle transition and G2/M transition

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1 (H1-0, SLBP Synthetic, Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-GFP11-Clathrin light chain

Plasmid

#70217

Purpose

Expresses GFP11-clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP11-Clathrin light chain (CLTA Human)

Tags

GFP11

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-GFP-LC3-RFP

Plasmid

#84573

Purpose

Expresses GFP-LC3-RFP in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

EGFP and mRFP1

Expression

Mammalian

Available Since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAED2

Plasmid

#234599

Purpose

Fluorescent reporter of the SOS response

Depositor

Tanel Tenson

Article

Kaldalu et al NPJ Antimicrob Resist. 2025 Apr 11;3(1):

Inserts

mScarlet-I
gfp-mut2

Use

Reporter

Expression

Bacterial

Mutation

GFPmut2 was derived from avGFP with the following…

Promoter

Ptet+dnaK P1 and cda

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-Beatrix

Plasmid

#167528

Purpose

Dual-color fluorescent reporter for the creation and detection of Cre-dependent tunable mosaics in floxed mouse lines and cells.

Depositor

Gian Michele Ratto

Article

Trovato et al Nat Commun. 2020 Dec 3;11(1):6194. doi:

Insert

Beatrix

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Promoter

CAG

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC589 - pAAV c-fos Nuc-eYFP

Plasmid

#59133

Purpose

An AAV vector that expresses nuclear localized eYFP under control of the c-fos promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Nuclear-localized Enhanced Yellow Fluorescent Protein

Use

AAV

Tags

3xNLS

Expression

Mammalian

Promoter

mFos

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-UBC-S1-GCaMP6f

Plasmid

#160722

Purpose

Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

S1-GCaMP6f

Use

AAV

Expression

Mammalian

Mutation

N/A

Promoter

Human ubiquitin C (UBC) promoter

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pOTTC364 - pAAV CaMKIIa iRFP

Plasmid

#47903

Purpose

An AAV vector that expresses iRFP under the CaM Kinase IIa promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Infrared fluorescent protein

Use

AAV

Expression

Mammalian

Promoter

CaMKIIa

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pSF11-wNIR-GECO2-T2A-HO1

Plasmid

#159606

Purpose

Codon-optimized near-infrared fluorescent calcium indicator NIR-GECO2 with codon-optimized heme-oxygenase in C.elegans expression plasmid

Depositor

Robert Campbell

Article

Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

Insert

wNIR-GECO2-T2A-HO1

Expression

Worm

Promoter

tag-168

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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Epac-S-H21

Plasmid

#170335

Purpose

CFP(ST)_EPAC full length_Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

Depositor

Kees Jalink

Article

Ponsioen et al EMBO Rep. 2004 Dec . 5(12):1176-80.

Insert

CFP(ST)_EPAC full length_Y(UST) (RAPGEF3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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Chicken Mermaid S188

Plasmid

#53617

Purpose

Genetically encoded voltage sensor based on mUKG-mKOk FRET pair

Depositor

Vincent Pieribone

Article

Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

Insert

Chicken Mermaid S188

Expression

Mammalian

Mutation

Gg-VSP contains an R153Q mutation and an amino ac…

Promoter

CMV

Available Since

Aug. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pOTTC588 - pAAV c-fos Nuc-iRFP

Plasmid

#59132

Purpose

An AAV vector that expresses nuclear localized iRFP under control of the c-fos promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

Nuclear-localized Infrared Fluorescent Protein

Use

AAV

Tags

3xNLS

Expression

Mammalian

Promoter

mFos

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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Epac-S-H27

Plasmid

#170336

Purpose

CFP(ST)_EPACdDEP _Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

Depositor

Kees Jalink

Article

Ponsioen et al EMBO Rep. 2004 Dec . 5(12):1176-80.

Insert

CFP(ST)_EPACdDEP _Y(UST) (RAPGEF3 Human)

Expression

Mammalian

Mutation

Deletion of DEP domain

Promoter

CMV

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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