We narrowed to 15,871 results for: nans
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Plasmid#163140PurposepLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only
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pLyGo-Ec-3
Plasmid#163129PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-5
Plasmid#163131PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Coh-ddFLN4-ELP(MV7E2)3-His-ybbR
Plasmid#157672PurposeExpression of Coh (type III from Ruminococcus flavefaciens) with ddFLN4, ELP linker, His6x tag, and ybbR Tag.DepositorInsertCohesin (R.f) with ddFLN4, ELP, Histag and ybbR tag
TagsddFLN4, ELP, Histag and ybbR tagExpressionBacterialPromoterT7Available SinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_vhhGFP4::Nrv1::tagBFP
Plasmid#163925PurposeExpression of GrabFP-B-extracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (extracellular) nanobody with Nrv1 protein and tagBFP fluorophor
UseCre/LoxTagstagBFPExpressionInsectAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
K8.1Pr-pGL4.16-OriLyt
Plasmid#131038PurposeFirefly Luciferase reporter for K8.1 promoter from KSHV with the lytic origin of replicationDepositorInsertKSHV Lytic Origin of replication
UseLuciferaseExpressionMammalianAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFB-U6-control-gRNA-hSyn-mCherry-WPRE-SV40pA
Plasmid#128347PurposepAAV encoding control gRNA for CRISPR gRNAs listed aboveDepositorInsertcontrol (negative)
UseCRISPRExpressionMammalianAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3)P Probe
Plasmid#211508PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteriaDepositorAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gγ2-LSS-mKATE2(N)
Plasmid#207160PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNADepositorAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
8138-E02
Plasmid#225659PurposeGateway clone for SORE6 reporter (6 repeats of a SOX2/OCT4 response element from the human NANOG promoter)DepositorInsertSORE6
UseGateway entry cloneAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV500 A-CREB
Plasmid#33371DepositorInsertCREB (Creb1 Mouse)
TagsFLAGExpressionMammalianMutationDominant Negative (see notes)PromoterCMVAvailable SinceDec. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1∆HisTag_hNMT_Nef
Plasmid#66078PurposeSingle vector system expressing hNMT1 (81-496 amino acids) and NEF allowing myristoylation of recombinant protein produce in Ecoli systemDepositorInsertsExpressionBacterialMutationDeletion of first 80 amino acidsPromoterT7-lacOAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPFO*
Plasmid#120400PurposeExpresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.DepositorInsertMutant form of Perfringolysin O
TagsHis6 tagExpressionBacterialMutationAmino acids 1-27 are deleted, starts at amino aci…Available SinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLIX403_Capture1_APOBEC_HA_P2A_mRuby
Plasmid#183901PurposeInducible lentiviral expression, TRE-gateway-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTADepositorTypeEmpty backboneUseLentiviral; Gateway destination vector, doxycycli…TagsCapture1_APOBEC_HA_P2A_mRubyExpressionMammalianPromoterTRE promoter, Tet ONAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMZ828
Plasmid#155322PurposeConstitutive expression of PhyB(1-650)-VP16 in plantsDepositorInsertPCaMV35S-PhyB(1-650)-VP16-NLS-Tnos
UseSynthetic BiologyExpressionPlantAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
(SUMO)10-(SIM)5
Plasmid#126866PurposeBacterial expression plasmid for multivalent scaffold of (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing client proteins.DepositorInsert10 repeats of human SUMO3 and 5 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
ExpressionBacterialPromotertacAvailable SinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-MYRIP
Plasmid#89586PurposeExpression plasmid for a positive control prey protein in NanoSPD 2.0 assays.DepositorAvailable SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG94-15
Plasmid#228971PurposeFor bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.DepositorInsertLaG94-15
Tags6xHISExpressionBacterialPromoterT7Available SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-SF3B1-K700E-reporter
Plasmid#200318PurposeA fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.DepositorInsertBSD-2A-EGFPi
UseLentiviralExpressionMammalianPromoterEF1-aAvailable SinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-2xSV40_NLS-NLuc
Plasmid#135953PurposeExpresses the NanoLuciferase targeted to the nucleus in mammalian cells.DepositorInsertNanoLuciferase
Tags2xSV40-NLSExpressionMammalianPromoterEF1aAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only