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pRK7-HA-S6K1-E389-deltaCT

Plasmid

#8993

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Tags

HA

Expression

Mammalian

Mutation

T389E, stop codon at amino acid 402

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pGEX6P-2-human RNF146 full length

Plasmid

#132610

Purpose

expresses human RNF146 full length in E. coli

Depositor

Wenqing Xu

Article

DaRosa et al Nature. 2015 Jan 8;517(7533):223-6. doi:

Insert

RNF146 (RNF146 Human)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pHCMV_DENV3_PrME

Plasmid

#207265

Purpose

Mammalian expression of Dengue virus 3 PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

DENV3_PrME (POLY Dengue virus 3)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-PTRE-tight-flex-hM3Dq-mCherry-WPRE-pA

Plasmid

#115161

Purpose

This plasmid is for use with neuronal cell-type selective activity tagging. The hM3Dq receptor expression requires both neural activity and Cre recombinase.

Depositor

William Wisden

Article

Harding et al Curr Biol. 2018 Jul 23;28(14):2263-2273.

Inserts

PTRE - tet activator responsive promoter
flex sequence
hM3dq-mCherry (inverted)
flex sequence

Use

AAV

Tags

hM3Dq-mCherry

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-FCGR1A-COMP5AP-AviTag-9xHis

Plasmid

#157282

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

FCGR1A (FCGR1A Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-FGFR2

Plasmid

#116741

Purpose

Lentiviral expression of FGFR2

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FGFR2 (FGFR2 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-HDAC4-FLAG

Plasmid

#30485

Depositor

Tso-Pang Yao

Insert

HDAC4 (HDAC4 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pLV UBC msTMEM115-3C-3xHA-P2A-eGFP

Plasmid

#214997

Purpose

Stably express the GolgiTag (GolgiIP handle) in mammalian cells (mouse ORF). eGFP for cell sorting desired expression levels.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

TMEM115 (Tmem115 Mouse)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Promoter

UBC

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pWSK29

Plasmid

#172972

Purpose

Low copy number cloning vector

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pEB1-mCerulean

Plasmid

#103968

Purpose

Plasmid encoding mCerulean

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mCerulean

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

ProC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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