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pCMU-PDESr

Plasmid

#61206

Purpose

Fluorescent reporter for plasmodesmata AtPDLP1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtPDLP1

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pET28-2H12

Plasmid

#221186

Purpose

His6-tagged cdGreen expressed from T7 promoter (Internal Lab ID: UJ11308)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen

Expression

Bacterial

Promoter

T7

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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3xmNeonGreen_UtrCH

Plasmid

#129607

Purpose

In vivo visualization of F-actin (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Utrophin

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Tubulin-hfYFP

Plasmid

#186515

Purpose

Localization of hyperfolder YFP fluorescent protein to tubulin

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Tags

Tubulin

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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dimericTomato-1xwGBD

Plasmid

#176099

Purpose

Visualization of active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WAS (WAS Human)

Use

Lentiviral

Promoter

CMVdel

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.nucGAP

Plasmid

#78736

Purpose

Expression of Ca2+ sensor GAP in the nucleus

Depositor

Teresa Alonso

Article

Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Insert

nucGAP

Tags

nucleoplasmin (Xenopus)

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
NAX_CAGGS_CAS9_mcherry

Plasmid

#167862

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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miniCMV eGFP hPGK BSD

Plasmid

#188519

Purpose

Backbone for CRISPR activation (CRISPRa) isogenic PAM testing

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Type

Empty backbone

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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p11d-sctRPA

Plasmid

#102614

Purpose

Expression of S. cerevisiea RPA in E.coli

Depositor

Marc Wold

Article

Sibenaller et al Biochemistry. 1998 Sep 8;37(36):12496-50

Inserts

RPA1 (RFA1 Budding Yeast)
RPA2 (RFA2 Budding Yeast)
RPA3 (RFA3 Budding Yeast)

Expression

Bacterial

Mutation

RFA2 has intron deleted

Promoter

T7

Available Since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pXylA-agrCA-I

Plasmid

#53437

Purpose

xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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