2,294 results

1. pFSW Syt1-C2B V304A,I367A IRES GFP

   Plasmid

   #133821

   Purpose

   Encodes the C2B domain of synaptotagmin 1 with membrane-penetrating residue mutations V304A,I367A for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Mutation

   V304A, I367A

   Promoter

   hSyn (human synapsin I promoter)

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFSW Syt1-C2B D363,365N IRES GFP

   Plasmid

   #133822

   Purpose

   Encodes the C2B domain of synaptotagmin 1 with calcium-coordinating ligand mutations D363,365N for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Mutation

   D363,365N

   Promoter

   hSyn (human synapsin I promoter)

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFSW Syt1-C2A K189-192A IRES GFP

   Plasmid

   #133823

   Purpose

   Encodes the C2A domain of synaptotagmin 1 with poly-lysine patch mutations K189-192A for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Mutation

   K189-192A

   Promoter

   hSyn (human synapsin I promoter)

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFSW Syt1-C2A D230,232N IRES GFP

   Plasmid

   #133824

   Purpose

   Encodes the C2A domain of synaptotagmin 1 with calcium-coordinating ligand mutations D230,232N for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Mutation

   D230,232N

   Promoter

   hSyn (human synapsin I promoter)

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFSW Syt1-C2A M173,F234A IRES GFP

   Plasmid

   #133825

   Purpose

   Encodes the C2A domain of synaptotagmin 1 with membrane-penetrating residue mutations M173,F234A for viral expression

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

   Insert

   Syt1 (Syt1 Rat)
   

   Use

   Lentiviral

   Tags

   IRES-GFP

   Mutation

   M173, F234A

   Promoter

   hSyn (human synapsin I promoter)

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMS22H-IRE

   Plasmid

   #133905

   Purpose

   Positive control when used in combination with pAWH-IRP. Expression of MS2BS-IRE site hybrid. Homology regions for recombination with pAWH

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Insert

   IRE (Ftl1 Rat)
   

   Expression

   Yeast

   Promoter

   Pol III

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX Syt1 C2AB (96-421) F234C

   Plasmid

   #170644

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Expression

   Bacterial

   Mutation

   C277A, F234C

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX Syt1 C2AB (96-421) V304C

   Plasmid

   #170645

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Expression

   Bacterial

   Mutation

   C277A, V304C

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX Syt1 C2AB (96-421) D303G F234C

   Plasmid

   #170647

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Expression

   Bacterial

   Mutation

   D303G, C277A, F234C

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX Syt1 C2AB (96-421) D303G V304C

    Plasmid

    #170648

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Expression

    Bacterial

    Mutation

    D303G, C277A, V304C

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX Syt1 C2AB (96-421) D365E F234C

    Plasmid

    #170650

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Expression

    Bacterial

    Mutation

    D365E, C277A, F234C

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX Syt1 C2AB (96-421) D365E V304C

    Plasmid

    #170651

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Expression

    Bacterial

    Mutation

    D365E, C277A, V304C

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX Syt1 C2AB (96-421) I367T F234C

    Plasmid

    #170653

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Expression

    Bacterial

    Mutation

    I367T, C277A, F234C

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX Syt1 C2AB (96-421) I367T V304C

    Plasmid

    #170654

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Expression

    Bacterial

    Mutation

    I367T, C277A, V304C

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge