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pBacPAK-HFT-dKDM4B

Plasmid

#20234

Depositor

Jerry Workman

Article

Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

Insert

dKDM4B (Kdm4B Fly)

Tags

FLAG, His, and TEV

Expression

Insect

Available Since

March 17, 2009

Availability

Academic Institutions and Nonprofits only
HOXB6-pSVL

Plasmid

#20995

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

HOXB6 (HOXB6 Human)

Expression

Mammalian

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
RFP->M71-IRES-M71-IRES-tauGFP ACNF TV

Plasmid

#15629

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):833-46.

Insert

RFP->M71-IRES-M71-IRES-tauGFP ACNF TV

Use

High copy number

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pSFV_(3'UTR)_RLEloop

Plasmid

#240253

Purpose

PROTEUS vector with no transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_luciferase

Plasmid

#240264

Purpose

Constitutive expression (CMV promoter) of firefly luciferase. Used as a control for luciferase assays.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

firefly luciferase

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pSpyB-Tn7sg

Plasmid

#248694

Purpose

sgRNA expression vector - pBG35 promoter with Tn7sg negative control sgRNA

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

Tn7sg

Expression

Bacterial

Promoter

BG35

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF19_KO_Puro_donor

Plasmid

#246401

Purpose

PHF19 KO donor plasmid with puromycin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF19 (PHF19 Human)

Expression

Mammalian

Mutation

KO

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_MTF2_KO_Puro_donor

Plasmid

#246393

Purpose

MTF2 KO donor plasmid with puromycin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

MTF2 (MTF2 Human)

Expression

Mammalian

Mutation

KO

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_MTF2_HTC3xF_BSD_donor

Plasmid

#246391

Purpose

MTF2 donor plasmid with blasticidin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

MTF2 (MTF2 Human)

Tags

HaloTag_3XFLAG

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF1_KO_Puro_donor

Plasmid

#246397

Purpose

PHF1 KO donor plasmid with puromycin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF1 (PHF1 Human)

Expression

Mammalian

Mutation

KO

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF19_HTC3xF_Puro_donor

Plasmid

#246399

Purpose

PHF19 donor plasmid with puromycin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF19 (PHF19 Human)

Tags

HaloTag_3XFLAG

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE20

Plasmid

#90362

Purpose

XBP1 - ER stress gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

XBP1

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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CBE-Cas12a-GFP

Plasmid

#246533

Purpose

Plant co-editing vector

Depositor

Nian Wang

Article

Huang et al Nat Plants. 2023 Oct;9(10):1591-1597. do

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

CmYLCV

Available Since

Nov. 3, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-Serbp1-meGFP

Plasmid

#241316

Purpose

mouse Serbp1 fused to meGFP for expression in mammalian cells

Depositor

Stavros Lomvardas

Insert

Serbp1 (Serbp1 Mouse)

Tags

meGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-Mex3a-mCherry

Plasmid

#241312

Purpose

Expression of Mex3a-mCherry fusion protein in mammalian cells

Depositor

Stavros Lomvardas

Insert

Mex3a (Mex3a Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE36

Plasmid

#90377

Purpose

STAT4 - Stat4 gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

STAT4

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE24

Plasmid

#90366

Purpose

HNF4 - Hepatocyte nuclear factor 4 gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

HNF4

Available Since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCXLE-hTBX3

Plasmid

#223196

Purpose

Episomal plasmid expresses hTBX3

Depositor

R. Michael Roberts

Insert

T-Box Transcription Factor 3 (TBX3 Human)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pDL1-Spol-Ovo-Nterm

Plasmid

#242942

Purpose

Encodes an N-terminal fragment of Spol-Ovo for use in generating dsRNA for RNAi in the planarian S. polychroa

Depositor

Erin Davies

Article

Deihl et al bioRxiv [Preprint]. 2025 Jul 22:2025.07.

Insert

Spol-Ovo (Nterm fragment)

Use

RNAi

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDL1-Spol-Ovo-C-term

Plasmid

#242943

Purpose

Encodes a C-terminal fragment of Spol-Ovo for use in generating dsRNA for RNAi in the planarian S. polychroa

Depositor

Erin Davies

Article

Deihl et al bioRxiv [Preprint]. 2025 Jul 22:2025.07.

Insert

Spol-Ovo (Cterm fragment)

Use

RNAi

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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C-term_aMFx1 (4a)

Plasmid

#238900

Purpose

Plasmid insert contains the aMFx1 C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

C-term_aMFx1

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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C-term_aMFx1_noKREAEA (4a)

Plasmid

#238901

Purpose

Plasmid insert contains the aMFx1 noKREAEA C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

C-term_aMFx1_noKREAEA

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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C-term_aMFx4 (4a)

Plasmid

#238902

Purpose

Plasmid insert contains the aMFx4 C-terminal tag sequence for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

C-term_aMFx4

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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twinStrep_FLAG_aMFx1 (4a)

Plasmid

#238904

Purpose

Plasmid insert contains the aMFx1, FLAG and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

twinStrep_FLAG_aMFx1

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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twinStrep_HA_aMFx1 (4a)

Plasmid

#238905

Purpose

Plasmid insert contains the aMFx1, HA and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

twinStrep_HA_aMFx1

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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twinStrep_aMFx1 (4a)

Plasmid

#238906

Purpose

Plasmid insert contains the aMFx1 and twinStrep C-terminal tag sequences for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

twinStrep_aMFx1

Use

Synthetic Biology

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pDRF1 Cyterm-mGold

Plasmid

#224084

Purpose

Expresses Cyterm-mGold to check for monomericity.

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026; doi: https://doi.o

Insert

Cyterm-mGold

Expression

Yeast

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pUBC(h)-DEST-mKOk

Plasmid

#224830

Purpose

Empty Gateway DEST vector for expressing C-terminal tagged proteins with mKusabiraOrangekand hygromycin selection in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Type

Empty backbone

Tags

mKusabiraOrangek

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEP17

Plasmid

#229234

Purpose

PutP (Sodium/proline symporter) under tet operator, TetR

Depositor

Yolanda Schaerli

Article

Pignon et al ISME J. 2025 Jun 13:wraf122. doi: 10.109

Insert

putP (Sodium/proline symporter) (putP E. coli)

Expression

Bacterial

Promoter

tet operator

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B0_M13ps

Plasmid

#231421

Purpose

M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B0

Use

Synthetic Biology

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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hPDZD11-HA

Plasmid

#236537

Purpose

For expression of PDZD11 (human) in mammalian cells

Depositor

Sandra Citi

Article

Guerrera et al J Biol Chem. 2016 May 20;291(21):11016-2

Insert

PDZD11 (PDZD11 Human)

Tags

HA

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_NOT_iC

Plasmid

#231418

Purpose

This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-C1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ290.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Inserts

dCas9
GFP

Use

Synthetic Biology

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pKrev-CGI

Plasmid

#225568

Purpose

packaging plasmid for lentivector production

Depositor

Ferdinando Pucci

Article

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Type

Empty backbone

Expression

Mammalian

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p48.unipolyA.dNGFR.mCMV.PGK.eGFP.Gem1.WPRE

Plasmid

#226767

Purpose

vector with NGFR and GFP

Depositor

Ferdinando Pucci

Article

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Type

Empty backbone

Expression

Mammalian

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p49.unipolyA.Cdt1.mSca.mCMV.PGK.eGFP.Gem1.WPRE

Plasmid

#226768

Purpose

vector with mScarlet fluorescent protein and GFP

Depositor

Ferdinando Pucci

Article

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Type

Empty backbone

Expression

Mammalian

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVA19_sgRNA-C1_M13ps

Plasmid

#231411

Purpose

M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C1

Use

Synthetic Biology

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVA47_dCas9-GFP_NOT_iA

Plasmid

#231417

Purpose

This NOT-gate plasmid expresses dCas9 from a constitutive promoter, and GFP from a promoter repressible by sgRNA-A1. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ285.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Inserts

dCas9
GFP

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEVA19_sgRNA-C0_M13ps

Plasmid

#231410

Purpose

M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV_Zdk3-VPR_P2A_dCas9-AsLOVx3-NLSx3

Plasmid

#231568

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

All-in-one LOOMINA construct

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
CMV_dCas9-AsLOV2x3-NLSx3

Plasmid

#231567

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

dCas9-AsLOV2x3

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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