We narrowed to 2,365 results for: DAP

1. pYI2165

   Plasmid

   #228347

   Purpose

   2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0171 expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   MFalpha(2xAdv)-LE-ALX-0171-3A-FLAG-His
   

   Use

   Affinity Reagent/ Antibody

   Tags

   AAA linker followed by FLAG-His6 and MF_ secretio…

   Expression

   Yeast

   Mutation

   Promoter

   2,4-Diacetylphloroglucinol-regulatable synthetic …

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-117G-hyeA3A-BE4max

   Plasmid

   #157946

   Purpose

   Lentiviral expression of hyeA3A-BE4max

   Depositor

   Dali Li

   Article

   Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

   Insert

   Lenti -117G-hyeA3A-BE4max
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   N57G

   Promoter

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOD1988-epiDEG

   Plasmid

   #89357

   Purpose

   MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pdpy-7 (epidermis specific).

   Depositor

   Karen Oegema

   Article

   Wang et al Development. 2017 Jun 15. pii: dev.15009

   Insert

   vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
   

   Use

   Targeting vector for mos1 transposon mediated sin…

   Tags

   vhhGFP4 (GFP nanobody)

   Expression

   Mutation

   Promoter

   Pdpy-7

   Available Since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. FKBP-beta2(616-951)-GFP

   Plasmid

   #100726

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP2B1 (AP2B1 Human)
   

   Use

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Mutation

   Long isoform

   Promoter

   CMV

   Available Since

   Sept. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-117G-hyA3A-BE4max

   Plasmid

   #157945

   Purpose

   Lentiviral expression of hyA3A-BE4max

   Depositor

   Dali Li

   Article

   Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

   Insert

   Lenti-117G- hyA3A-BE4max
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-2X-P2A-Puro

   Plasmid

   #110840

   Purpose

   Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA-2X
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A and 2 tandem NLS sequences in the XTEN linker

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-BE4GamRA-P2A-Puro

   Plasmid

   #112673

   Purpose

   Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE4GamRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   NLS sequence at the N-terminus and D10A

   Promoter

   EF1s

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST17-cDAXX

   Plasmid

   #169729

   Purpose

   Bacterial expression of N-terminally 6His tagged cDAXX

   Depositor

   Tanja Mittag

   Article

   Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

   Insert

   DAXX (DAXX Human)
   

   Use

   Tags

   6 His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-xCas9RA-P2A-Puro

   Plasmid

   #110871

   Purpose

   Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)RA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA6.2EmGFP-cDAXX

    Plasmid

    #169727

    Purpose

    Mammalian expression construct of GFP tagged cDAXX

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    cDAXX (DAXX Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Comprises residues 495-740

    Promoter

    CMV

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. FKBP-beta3(702-1094)-GFP

    Plasmid

    #100734

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP3B1 (AP3B1 Human)
    

    Use

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    appendage and ear only

    Promoter

    CMV

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. FKBP-beta2(616-951)-mRuby2

    Plasmid

    #100730

    Purpose

    To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    AP2B1 (AP2B1 Human)
    

    Use

    Tags

    FKBP and mRuby2

    Expression

    Mammalian

    Mutation

    Long isoform

    Promoter

    CMV

    Available Since

    Sept. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-BE4RA-P2A-Puro

    Plasmid

    #112672

    Purpose

    Lentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE4RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    EF1s

    Available Since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-BE3RA-P2A-GFP-PGK-Puro

    Plasmid

    #110868

    Purpose

    Lentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pOD2087-trnDEG

    Plasmid

    #89369

    Purpose

    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmec-18 (touch neuron specific).

    Depositor

    Karen Oegema

    Article

    Wang et al Development. 2017 Jun 15. pii: dev.15009

    Insert

    vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)
    

    Use

    Targeting vector for mos1 transposon mediated sin…

    Tags

    vhhGFP4 (GFP nanobody)

    Expression

    Mutation

    Promoter

    Pmec-18

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-HF1RA-PGK-Puro

    Plasmid

    #110860

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5-H1-shRNA-CMV-HA-PACSIN1

    Plasmid

    #72577

    Purpose

    Bicistronic construct expressing PACSIN1 shRNA and HA-PACSIN1-rescue construct

    Depositor

    Richard Huganir

    Article

    Anggono et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

    Inserts

    PACSIN1 (Pacsin1 Mouse)
    H1-PACSIN1 shRNA cassette
    

    Use

    RNAi

    Tags

    HA

    Expression

    Mammalian

    Mutation

    5 silent mutations around shRNA recognition seque…

    Promoter

    CMV and H1

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-HF1RA-P2A-Puro

    Plasmid

    #110853

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-BE3-P2A-GFP-PGK-Puro

    Plasmid

    #110867

    Purpose

    Lentiviral vector for constitutive expression of BE3-P2A-GFP (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-VQRRA-P2A-Puro

    Plasmid

    #110851

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VQRRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VQRRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D1135V, R1335Q, T1337R and NLS sequence at the N-…

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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