We narrowed to 8,624 results for: FIE

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pBS-KS-attB2-SA(2)-T2A-Gal4DBD-Hsp70

Plasmid

#62904

Purpose

Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4DBD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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G10 Cas9 MDC123

Plasmid

#59187

Purpose

G10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selection

Depositor

Robert Stupar

Article

Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

Insert

Glycine Max codon optimized Cas9

Use

CRISPR

Expression

Plant

Promoter

G10

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-LexA::QFAD-Hsp70

Plasmid

#62949

Purpose

Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-LexA::QFAD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBITE-EGFRvIII-F265-OKT3

Plasmid

#190679

Purpose

Encodes an EGFRvIII-CD3 BITE protein (F265 clone)

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Anti-EGFRvIII-scFv-F265-modularized-OKT3-HisTag

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EFS

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-JEDI-1P-WPRE

Plasmid

#202613

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSyn

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-DIO-APEX2NES

Plasmid

#79907

Purpose

Adeno-associated virus plasmid, Cre-dependent FLEX switch, APEX2NES

Depositor

Joshua Sanes

Article

Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

Insert

APEX2NES

Use

AAV and Cre/Lox

Tags

V5

Expression

Mammalian

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pET151-GyrA

Plasmid

#216229

Purpose

S. aureus USA300 GyrA Codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDuRCC-K

Plasmid

#81193

Purpose

Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance

Depositor

Eckhard Boles

Article

Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

Inserts

Cas9
empty gRNA cassette
empty gRNA cassette

Use

CRISPR

Tags

NTS

Expression

Yeast

Mutation

Please see depositor comments below., Please view…

Promoter

ROX3 and SNP52p

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pMP71-tCD19-SIINFEKL

Plasmid

#174601

Purpose

expresses the extracellular and transmembrane domain of murine CD19 with a C terminal fusion of the SIINFEKL epitope

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

membrane bound CD19, SIINFEKL (ovalbumin epitope)

Expression

Mammalian

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCB036 FoMV-sgGFP

Plasmid

#234813

Purpose

To express gRNA from Foxtail Mosaic Virus Vector targeting GFP (T-DNA)

Depositor

Daniel Voytas

Article

Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

Insert

gRNA targeting GFP

Expression

Plant

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pUASp-GBP-Tbro

Plasmid

#179995

Purpose

Expresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASp version is ideal for expression in the germline.

Depositor

Graydon Gonsalvez

Article

Baker et al Development. 2021 Nov 15;148(22). pii: 2

Insert

GBP nanobody

Tags

Trbo

Expression

Insect

Promoter

UAS-Gal4

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Escherichia_coli_SLK172

Plasmid

#224395

Purpose

For expression of the type I CBASS system encoded by Escherichia coli SLK172

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

EcCdnD_4TM

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLD-puro-2A-rtTA-TcVA

Plasmid

#24592

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only

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p3E-Rac1-DN

Plasmid

#109582

Purpose

Multisite gateway vector for 3' tagging with dominant negative Rac1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rac1 (rac1a Zebrafish)

Use

Zebrafish plasmids

Mutation

dominant negative

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-3X Gal80-Hsp70

Plasmid

#62951

Purpose

Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 0. Gal80 is tagged with FLAG, HA and EGFP.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

3XGal80-FLAG/HA/EGFP-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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SPDK3860

Plasmid

#199245

Purpose

Modified Tobacco rattle virus (TRV) RNA2 vector for editing of Nicotiana benthamiana Phytoene desaturase (PDS) gene

Depositor

Savithramma Dinesh-Kumar

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence

Use

T-dna vector

Promoter

pPEBV

Available Since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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p405-BrdU-Inc

Plasmid

#71791

Purpose

BrdU-incorporating plasmid with selectable marker LEU2

Depositor

Oscar Aparicio

Article

Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

Inserts

Thymidine Kinase
ENT1 (SLC29A1 Human)

Expression

Yeast

Promoter

ADH1 and GPD

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV_hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-Thy1.1

Plasmid

#187925

Purpose

sgRNA vector backbone expressing puromycin resistance and CD90.1 as selection markers

Depositor

Martin Turner

Article

Turner et al eLife. 2022 Apr 22;11. pii: 72313. doi:

Insert

hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-CD90.1

Use

CRISPR and Retroviral

Expression

Mammalian

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-U6sgp53-U6sgbbSapI-GFAPCre

Plasmid

#100275

Purpose

AAV-CRISPR mediated genome editing in glia / astrocytes

Depositor

Sidi Chen

Article

Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

Type

Empty backbone

Use

AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

Expression

Mammalian

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-Rac1

Plasmid

#109581

Purpose

Multisite gateway vector for 3' tagging with Rac1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rac1 (rac1a Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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