We narrowed to 5,111 results for: codon optimized

1. p32Fn

   Plasmid

   #158153

   Purpose

   Construct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and LbCpf1, respectively. For plant genome editing.

   Depositor

   Kabin Xie

   Article

   Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Mutation

   Rice codon optimized

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHCMV_CHHF_GPC

   Plasmid

   #207260

   Purpose

   Mammalian expression of Chapare hemorrhagic fever virus GPC protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   CHHF_GPC (CVsS_gp1 Chapare hemorrhagic fever virus)
   

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTU424

   Plasmid

   #178983

   Purpose

   pACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized)

   Depositor

   Stan Brouns

   Article

   van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

   Insert

   TPR-CHAT
   

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2+_NLS-Tir1-HA

   Plasmid

   #117716

   Purpose

   mRNA synthesis of NLS-TIR1-HA for injection

   Depositor

   Joerg Mansfeld

   Article

   Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

   Insert

   TRANSPORT INHIBITOR RESPONSE 1
   

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   codon-optimized TIR1 for murine expression

   Promoter

   SP6

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAS9-TPC

   Plasmid

   #61478

   Purpose

   Binary expression vector with A.th. codon-optimized Cas9 for conventional cloning

   Depositor

   Holger Puchta

   Article

   Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   PcUbi4-2

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. His-Tev-WWP2

   Plasmid

   #186807

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-WWP2.

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   His-Tev-WWP2 (WWP2 Human)
   

   Tags

   His6-Tev

   Expression

   Bacterial

   Available Since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. ALIX-His(1-868, P801G)

   Plasmid

   #186808

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses ALIX-His(1-868, P801G).

   Depositor

   Lalit Deshmukh

   Article

   Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

   Insert

   ALIX-His (1-868, P801G) (PDCD6IP Human)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Proline 801 to Glycine

   Available Since

   March 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPR - AAVS1 sgRNA

   Plasmid

   #70661

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backbone

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against AAVS1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHCMV_SLEV_PrME

   Plasmid

   #207309

   Purpose

   Mammalian expression of St. Louis encephalitis virus PrME protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   SLEV_PrME (POLY St. Louis encephalitis virus)
   

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYZ959

    Plasmid

    #192603

    Purpose

    TEF1p-Cas9(noBsmBI)-CYC1t and tRNA-GFP-SUP4t with CEN-URA3

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    Cas9 (codon optimized for human)
    

    Use

    Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Promoter

    ScTEF1

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas3

    Plasmid

    #229538

    Purpose

    pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Insert

    Cas3
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET32-sLatherin

    Plasmid

    #61685

    Purpose

    bacterial expression of the horse sweat/saliva protein Latherin as a His-tagged thioredoxin fusion

    Depositor

    Brian Smith

    Article

    Vance et al J R Soc Interface. 2013 Jun 19;10(85):20

    Insert

    Latherin (LATH horse)
    

    Tags

    HIS and Trx-Tag thioredoxin protein

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pSBY3_ligD

    Plasmid

    #104624

    Purpose

    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD

    Depositor

    Sheng Yang

    Article

    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

    Insert

    FnCpf1
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    FnCpf1 codon-optimized C.glutamicum

    Promoter

    hsp60

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. p1195-pssAAV.U1a.hNme2Cas9

    Plasmid

    #129534

    Purpose

    AAV vector expressing Nme2Cas9

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Insert

    human codon-optimized Nme2Cas9
    

    Use

    AAV

    Tags

    2xNLS and NLS-3xHA-NLS

    Promoter

    U1a

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBLO1811_Cas9_noNLS_human

    Plasmid

    #74490

    Purpose

    Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    Cas9
    

    Expression

    Mammalian

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAP24

    Plasmid

    #70190

    Purpose

    Modular vector for secreted luciferase reporter expression

    Depositor

    Wiep Klaas Smits

    Article

    Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

    Insert

    sLucopt
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Ptet

    Available Since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENO1-iRFP-NATr

    Plasmid

    #129731

    Purpose

    Integration plasmid to overexpress iRFP in Candida albicans

    Depositor

    Robert Wheeler

    Article

    Bergeron et al Infect Immun. 2017 Oct 18;85(11). pii: I

    Insert

    iRFP-Candida albicans codon-optimized
    

    Expression

    Yeast

    Promoter

    ENO1 from Candida albicans

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. p415TEF cyto roGFP2-Grx1(yeast)

    Plasmid

    #65004

    Purpose

    Yeast expression plasmid for roGFP2-Grx1; yeast codon optimized roGFP; yeast Grx1

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

    Insert

    Glutaredoxin-1
    

    Tags

    roGFP2

    Expression

    Yeast

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. C1-Lyn-mCitrine-EEVEE-LifeAct

    Plasmid

    #155226

    Purpose

    Alternate MPAct using Lyn/LifeAct instead of CaaX/F-tractin

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    Lyn11 fused to mCitrine C-term tagged with a codon optimized EEVEE and LifeAct
    

    Tags

    mCitrine

    Expression

    Mammalian

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ151

    Plasmid

    #69302

    Purpose

    Gateway entry vector with pcoCas9D10A

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pcoCas9D10A (Plant codon-optimized)
    

    Use

    CRISPR

    Tags

    2XFLAG

    Mutation

    D10A mutation; NLS fusion

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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