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pPBC-G6s

Plasmid

#62812

Purpose

pPBC-G6s expresses PiggyBac inverted terminal repeat-flanked GCaMP6s under the control of the CAG promoter.

Depositor

Karen Wilcox

Article

Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

Insert

ITR-CAG-GCaMP6s-ITR

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-gp78c/2767

Plasmid

#11429

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

gp78c (AMFR Human)

Tags

GST

Expression

Bacterial

Mutation

n/a

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
pECE M2-SNX17 WT

Plasmid

#69811

Purpose

Expresses M2-SNX17 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Sorting nexin-17 (SNX17 Human)

Tags

FLAG

Expression

Mammalian

Promoter

SV40 early promoter

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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IDE-2Strep

Plasmid

#210355

Purpose

Expresses IDE fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

IDE (IDE Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pMuLE Lenti Dest Neo

Plasmid

#62178

Purpose

MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses Neomycin cassette under the control of a PGK promoter. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Lentiviral; Mule gateway destination vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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"AND-gate" receiver plasmid

Plasmid

#127676

Purpose

Encodes the transcriptional activator LuxR and GFP. LuxR has an pLlacO-1 promoter and the GFP has a pLux promoter.

Depositor

Friedrich Simmel

Article

Schwarz-Schilling et al Integr Biol (Camb). 2016 Apr 18;8(4):564

Insert

LuxR, GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLlacO-1 for LuxR, pLux for GFP

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-KG-Itch/2793

Plasmid

#11434

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

Itch (Itch Mouse)

Tags

GST

Expression

Bacterial

Mutation

n/a

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf GFP-vcla

Plasmid

#185410

Purpose

GFP tagged form of zebrafish vinculin a. For in vitro transcription (SP6) or expression through CMV

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

vinculin a (vcla Zebrafish)

Use

In vitro synthesis of mrna

Tags

eGFP

Expression

Mammalian

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-OF (mutant TCF-4 sites)

Plasmid

#16559

Depositor

Bert Vogelstein

Article

Shih et al Cancer Res. 2000 Mar 15. 60(6):1671-6.

Insert

TCF-4 binding sites (TCF4 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

3 copies of mutant Tcf-4 binding sites

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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Fireworks RFP[PTC-] (AVA2515)

Plasmid

#85443

Purpose

Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.

Depositor

Joan Steitz

Article

Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

Insert

tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-HygromycinR

Use

Minimal backbone fragment for low-copy bacterial …

Expression

Mammalian

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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