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Plasmid#78338PurposeOverexpression of human PAX7 ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporterDepositorInsertPAX7 (PAX7 Human)
UseTags3x HA tag and IRES-hrGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
hGli2 flag3x
Plasmid#84920Purposemammalian expression of Gli2DepositorInsertGli2 (GLI2 Human)
UseTags3xFlagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-rM3D(Gs)-mCherry
Plasmid#50458PurposeDouble floxed Gs-coupled rM3D DREADD fused with mCherry under the control of human synapsin promoterDepositorHas ServiceAAV8InsertrM3D-mCherry
UseAAVTagsHA and mCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV-CaMKIIa-GCaMP6s-P2A-nls-dTomato
Plasmid#51086PurposeForebrain principle neuron specific expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophoreDepositorInsertGCaMP6s
UseAAVTagsP2A-nls-dTomatoExpressionMammalianMutationPromoterCaMKIIa 1.3 kbAvailable sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
MAP3K15-TAP
Plasmid#69728PurposeExpress tandem tagged MAP3K15 in cellsDepositorInsertApoptosis signal-regulating kinase 3 (MAP3K15 Human)
UseTagsHA-Myc tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pINDUCER20-hMYOD1-3xHA (#730)
Plasmid#78328PurposeDoxycycline-inducible lentiviral overexpression of human MYOD1 ORF with a 3xHA tagDepositorInsertMYOD1 (MYOD1 Human)
UseLentiviralTags3x HA tagExpressionMutationPromoterTRE2Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Con/Foff DREADD Gi-mCherry
Plasmid#177673PurposeExpresses DREADD hM4D (Gi) only in Cre expressing cells and not in Flp expressing cellsDepositorHas ServiceAAV8InsertDREADD Gi-mCherry
UseAAV, Cre/Lox, and Mouse Targeting ; IntrsectTagsmCherryExpressionMammalianMutationPromoternEFAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N3 alpha-SSeCKS
Plasmid#61294PurposeMammalian expression of GFP-alpha-SSeCKSDepositorInsertalpha SSeCKS (Akap12 Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pdsRED-Mono-N1-FoxO1
Plasmid#34678DepositorInsertFoxO1-RFP (Foxo1 Mouse)
UseTagsFlag and RFPExpressionMutationPromoterCMVAvailable sinceMarch 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-FLEX-SaCas9-U6-sgOprk1
Plasmid#159903PurposeMutagenesis of Oprk1DepositorInsertOprk1 (Oprk1 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
hMYOD1-3xHA-IRES-hrGFP (#343)
Plasmid#78327PurposeOverexpression of human MYOD1 ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporterDepositorInsertMYOD1 (MYOD1 Human)
UseTags3x HA tag and IRES-hrGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato
Plasmid#51082PurposeCre dependent expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophoreDepositorHas ServiceAAV1InsertGCaMP6s
UseAAVTagsP2A-nls-dTomatoExpressionMammalianMutationPromoterhEF1aAvailable sinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pLKO-Tet-ON-shCtrl (neo)
Plasmid#192076PurposeLentivirus for Dox-inducible expression of a non-targeting control shRNADepositorInsertnon-targeting randomized sequence
UseLentiviralTagsExpressionMutationPromoterH1 / Tet-responsive elementAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 H2B-mIFP T2A Mpro (wt)
Plasmid#163079PurposeExpresses wild type Mpro (active) and a near-infrared fluorescent protein mIFP fused to H2BDepositorInsertWild type Mpro of SARS-CoV-2 and H2B-mIFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Aiolos in Artichoke
Plasmid#74452PurposeMonitor post-translational degradation of Aiolos (Lentiviral, SFFV.Aiolos-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)DepositorInsertAiolos (IKZF3) (IKZF3 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterSFFV and EF1aAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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PX408 Francisella tularensis subsp. novicida Cas9
Plasmid#68705PurposeFrancisella tularensis subsp. novicida Cas9DepositorInsertFtCas9
UseCRISPRTagsHA-NLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV asyn S129A
Plasmid#36066DepositorInsertalpha-synuclein S129A (SNCA Human)
UseAAVTagsExpressionMammalianMutationS129APromoterCMVAvailable sinceSept. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCB301TOR
Plasmid#164798PurposeOomycete expression vector using Agrobacterium-mediated transformationDepositorInsertNPTII
UseOomycete expressionTagsExpressionMutationPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HshnRNPM
Plasmid#64924PurposeExpresses V5- and SBP-tagged hnRNPM in mammalian cellsDepositorInserthnRNPM (HNRNPM Human)
UseTagsV5 and SBPExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-con/fon DREADD Gi-mCherry
Plasmid#200660PurposeConstruct for chemogenetically silencing and visualizing subpopulations of neurons in the retinaDepositorInsertDREADD Gi-mCherry
UseAAV, Cre/Lox, and Mouse Targeting ; IntrsectTagsmCherryExpressionMammalianMutationPromoterhSynAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsRRP41
Plasmid#64871Purposeexpresses V5- and SBP-tagged RRp41 in mamalian cellsDepositorInsertRRP41 (EXOSC4 Human)
UseTagsV5 and SBPExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20-FLAG-hMYOD1 (#800)
Plasmid#78330PurposeDoxycycline-inducible lentiviral overexpression of human MYOD1 ORF with a FLAG tagDepositorInsertMYOD1 (MYOD1 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterTRE2Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-DIO-TC66T-2A-EGFP-2A-oG
Plasmid#178429PurposeExpresses TVA(TC66T), EGFP, and optimized Rabies G (oG) protein in a FLEX cassetteDepositorInsertsTVA950 Glu66→Thr
Optimized G
EGFP
UseAAV and Cre/Lox; Adeno-associated virusTagsExpressionMutationPromoterhSynAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-CaMKIIa-GCaMP6f-P2A-nls-dTomato
Plasmid#51087PurposeForebrain principle neuron specific expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophoreDepositorInsertGCaMP6f
UseAAVTagsP2A-nls-dTomatoExpressionMammalianMutationPromoterCaMKIIa 1.3 kbAvailable sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsRRP4
Plasmid#64870Purposeexpressses V5- and SBP-tagged RRP4 in mammalian cellsDepositorInsertRRP4 (EXOSC2 Human)
UseTagsV5 and SBPExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-FLAG-dTomato-centrin-1
Plasmid#73332PurposeLentiviral vector encoding centrin-1 with an N-terminal FLAG and dTomato tagDepositorInsertcentrin-1 (CETN1 Human)
UseLentiviralTagsFLAG and dTomatoExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQXCINeo-LOX-BC
Plasmid#134762Purposea retroviral vector to express lysyl oxidase (derived from a cancer cell line)DepositorInsertLOX (LOX Human)
UseRetroviralTagsExpressionMammalianMutationR158Q relative to NP_002308.2PromoterCMVAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
piggyBAC-BRD4-Flag
Plasmid#214432PurposeA piggyBAC based plasmid for the stable expression of BRD4 with a flag tag.DepositorInsertBrd4 bromodomain containing 4 (Brd4 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterEF1Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNHA_SBV_NSs
Plasmid#208932PurposeExpresses N-terminally HA-tagged Schmallenberg orthobunyavirus NSs protein from CMV promoter in mammalian cellsDepositorInsertNSs
UseTagsHAExpressionMammalianMutationDeleted 50 bp from 5' end (including start c…PromoterCMVAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
zcUAS:PSD95.FingR-GFP-ZFC(CCR5TC)-KRAB(A)
Plasmid#72638PurposeTol2 based UAS modified with a CCR5TC binding site upstream of it, driving PSD95-FingR fused to GFP, CCR5TC zinc finger, and KRAB(A) repressor domainDepositorInsertUAS upstream of PSD95-FingR with ZFC(CCR5TC) and KRAB(A)
UseGatewayTagsExpressionMutationPromoterAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR221 LRRK2 wt
Plasmid#39529DepositorInsertLRRK2 (LRRK2 Human)
UseGateway compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-HA_human_BAP1
Plasmid#158109PurposeExpresses tagged BAP1, neomycin selectionDepositorInsertBAP1 (BAP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
SETD1A
Plasmid#32868PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSETD1A (SETD1A Human)
UseTagsHis tag (with TEV cleavage)ExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
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RHPMP125 (mEOS3.1-alpha Tubulin)
Plasmid#88834PurposeLocalization by Super-Resolution microscopy of alpha-Tubulin fused to mEOS3.1DepositorInsertalpha-tubulin (TUBA1B) (TUBA1B Human)
UseTagsmEOS3.1ExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only