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T7-VEE-CFP

Plasmid

#193357

Purpose

Self-replicating RNA vector expressing CFP fluorescent protein + PuroR

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

CFP

Use

Venezuelan equine encephalitis (vee) virus rna re…

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1α-ZC3H12D-D95N-IRES-EGFP

Plasmid

#153071

Purpose

Lentiviral vector expressing human ZC3H12D mutated (D95N)

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

Mutated ZC3H12D (D95N) (ZC3H12D Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Changed D95 to N, known to abrogate the RNase enz…

Promoter

EF1α

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLR5-CBh-dCas9-hEzh2-IRES-Hyg

Plasmid

#122375

Purpose

Expresses dCas9-hEzh2-IRES-Hyg in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

CBh-dCas9-hEzh2-IRES-Hyg-pA

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAFW-Ago2

Plasmid

#50554

Purpose

Expression of FLAG tagged Ago2 in S2 cell

Depositor

Yukihide Tomari

Article

Iwasaki et al Mol Cell. 2010 Jul 30;39(2):292-9. doi:

Insert

Argonaute2 (AGO2 Fly)

Use

Tags

FLAG

Expression

Insect

Mutation

Promoter

Actin5C

Available since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pF152 pcDNA3.1(+)SOD1A4V

Plasmid

#26398

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Use

Tags

Expression

Mammalian

Mutation

A4V

Promoter

Available since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only
pF154 pcDNA3.1(+)SOD1G85R

Plasmid

#26400

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Use

Tags

Expression

Mammalian

Mutation

G85R

Promoter

Available since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only
pF148 pSOD1G37RAcGFP1

Plasmid

#26409

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

G37R

Promoter

Available since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only
pAc-GFP-dSUMO

Plasmid

#85696

Purpose

expression of GFP-SUMO1 in Drosophila cells

Depositor

Giannino Del Sal, Licio Collavin

Article

Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

Insert

SUMO (Sumo Fly)

Use

Tags

GFP

Expression

Insect

Mutation

Promoter

Ac5

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only
tet H-ras val12

Plasmid

#8900

Depositor

Lynda Chin

Article

Chin et al Nature. 1999 Jul 29. 400(6743):468-72.

Insert

ras val12 (HRAS Human)

Use

Tags

Tet-op promoter

Expression

Mammalian

Mutation

G12V

Promoter

Available since

Jan. 13, 2006

Availability

Academic Institutions and Nonprofits only
pF153 pcDNA3.1(+)SOD1G37R

Plasmid

#26399

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Use

Tags

Expression

Mammalian

Mutation

G37R

Promoter

Available since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only
pET28a-baPrs-hdNadV

Plasmid

#91950

Purpose

pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi

Depositor

George Cătălin Marinescu

Article

Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

Inserts

Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
Phosphoribosyl Pyrophosphate Synthetases

Use

Tags

Expression

Bacterial

Mutation

changed Leucine 135 to Isoleucine

Promoter

T7

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST47-CYFIP2.GFP

Plasmid

#109140

Purpose

GFP-tagged CYFIP2 expression construct

Depositor

Josef Kittler

Article

Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

Insert

CYFIP2 (CYFIP2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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px335-EF-Slc2a3-L

Plasmid

#122304

Purpose

Expresses sgRNA targeting mouse Slc2a3 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Slc2a3 (Slc2a3 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-ms-Klf1

Plasmid

#50785

Purpose

retroviral expression of mouse Klf1

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Klf1 (Klf1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pBactin-mNeonGreen-CI

Plasmid

#196850

Purpose

Expression of green fluorescent reporter mNeonGreen driven by the strong Beta-actin promoter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mNeonGreen

Use

Tags

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pME-foxc1a

Plasmid

#132971

Purpose

full-length of foxc1a without stop codon in pmE Gateway vector

Depositor

Nathan Lawson

Article

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

foxc1a (foxc1a Zebrafish)

Use

Gateway vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC-Myc-TRFcT

Plasmid

#44573

Depositor

Eros Lazzerini Denchi

Article

Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

Insert

chimeric TRF (TERF2, TERF1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

TRF2 where TRFH domain (aa42-241) has been replac…

Promoter

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only
px335-sgRNA-EF(Cas9wt)-Rif1

Plasmid

#122306

Purpose

Expresses sgRNA targeting mouse Rif1 and Cas9 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Rif1 (Rif1 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEcRNAP5

Plasmid

#128939

Purpose

pET21a derivative encoding E. coli core RNA polymerase subunits: RpoAppx234-241-(His)10, B, C, Z

Depositor

Seth Darst

Article

Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:

Inserts

RpoAppx234-241-(His)10 (rpoA Escherichia coli)
RpoB
RpoC
RpoZ

Use

Tags

His

Expression

Bacterial

Mutation

Residues 234-241 are substituted for a PreScissio…

Promoter

T7

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pBB0323-TEV-His12

Plasmid

#137039

Purpose

expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66700.1

Use

Tags

His12 (TEV protease cleavable)

Expression

Bacterial

Mutation

wild type, full-length protein, contains signal p…

Promoter

T7

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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