We narrowed to 18,240 results for: gateway

1. pMEF2c-AHF-DreERT2 (JDW 196)

   Plasmid

   #242586

   Purpose

   The Mef2c anterior heart field promoter driving expression of a tamoxifen inducible Dre recombinase.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   DreERT2
   

   Expression

   Mammalian

   Promoter

   mouse Mef2c

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGEN-Actin-Vhh-mNeonGreen-HA-pA (JDW 1308)

   Plasmid

   #242589

   Purpose

   A CAGGS driven expression vector containing an actin nanobody fused to mNeonGreen to label actin.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Actin-Vhh (actin nanobody)
   

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Sept. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR221-ITGB1(no stop)

   Plasmid

   #215453

   Purpose

   Gateway entry vector with integrin beta1 wild-type (WT) without a stop codon

   Depositor

   Johanna Ivaska

   Article

   Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

   Insert

   ITGB1 (ITGB1 Human)
   

   Use

   Gateway entry vector

   Mutation

   Silent mutations added to disrupt shRNA binding a…

   Promoter

   none

   Available Since

   Sept. 4, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pB-2x-lns-DEST (JDW 1206)

   Plasmid

   #229818

   Purpose

   A PiggyBac destination vector compatible with gateway with 2 core cHS4 insulators by each ITR site

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   July 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGEN nls-BFP-FLAG-WPRE-bgh-pA (JDW 452)

   Plasmid

   #229827

   Purpose

   A CAGGS driven nls BFP with a FLAG tag followed by a WPRE-bGH polyA for stable transcripts

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   3xnls-TagBFP2-MCS-FLAG
   

   Expression

   Mammalian

   Available Since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB-Puro-DEST (JDW 925)

   Plasmid

   #229808

   Purpose

   A puromycin selectable, PiggyBac destination vector compatible with 4-way multi-site gateway cloning system

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTol2-Ubi-lexPR-nls-GFP-pA (JDW 1316)

   Plasmid

   #229811

   Purpose

   A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus (1st generation)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LexA transactivator-LexA-Operon-nls-GFP
   

   Use

   Tol2 based expression vector

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)

   Plasmid

   #229819

   Purpose

   A gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Type

   Empty backbone

   Use

   Tol2 destination vector

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

   Plasmid

   #229823

   Purpose

   A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Luciferase-P2A-H2A-mCherry
   

   Expression

   Mammalian

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBeta-MHC-promoter DV (JDW 26)

    Plasmid

    #229835

    Purpose

    A gateway compatible destination vector containing the murine beta-MHC promoter for embryonic and postnatal cardiomyocyte expression.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAGEN-DV-IRES-myr-BFP (JDW 491)

    Plasmid

    #229834

    Purpose

    A CAGGS driven gateway compatible destination vector that contains an IRES-myristoylated-TagBFP for labeling the cell membrane

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 945)

    Plasmid

    #229837

    Purpose

    A Piggybac compatible, tet-on, NEO selectable, vector for expressing luciferase followed by a P2A cleavage peptide and H2A fused mCherry.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase
    

    Expression

    Mammalian

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 1154)

    Plasmid

    #229840

    Purpose

    A Piggybac compatible, tet-on, dual reporter encoding luciferase followed by a P2A cleavage peptide and then an H2A fused mCherry for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase
    

    Expression

    Mammalian

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. p5E-hs_Cldn5 (JDW 1120)

    Plasmid

    #224481

    Purpose

    Gateway compatible 5' entry clone containing the Human Claudin 5 promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Claudin-5 promoter
    

    Expression

    Mammalian

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221-DDX5(PrD)

    Plasmid

    #166167

    Purpose

    Gateway entry vector for N. furzeri DDX5-PrD(535-634) mutant

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    DDX5-PrD(535-634)
    

    Use

    Gateway entry vector

    Mutation

    PrD only 1606-1902

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR221-DDX5ΔPrD

    Plasmid

    #166164

    Purpose

    Gateway entry vector for N. furzeri DDX5ΔPrD(1-535) mutant

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    DDX5ΔPrD(1-535)
    

    Use

    Gateway entry vector

    Mutation

    deletion of 1606-1902

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221-DDX5ΔIDR

    Plasmid

    #166165

    Purpose

    Gateway entry vector for N. furzeri DDX5ΔIDR(1-483) mutant

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    DDX5ΔIDR(1-483)
    

    Use

    Gateway entry vector

    Mutation

    deletion of 1450-1902

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221-DDX5(IDR)

    Plasmid

    #166166

    Purpose

    Gateway entry vector for N. furzeri DDX5-IDR(483-634) mutant

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    DDX5-IDR(483-634)
    

    Use

    Gateway entry vector

    Mutation

    IDR only 1450-1902

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-Creb5(T59/T61A)(HA)

    Plasmid

    #195025

    Purpose

    Gateway vector containing HA-tagged Creb5 with T59/T61A mutation

    Depositor

    Cheng-Hai Zhang, Andrew Lassar

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    HA-tagged Creb5 (full length, 508aa; with with T59/T61A mutation) (CREB5 Bovine)
    

    Use

    Gateway cloning

    Tags

    3xHA

    Mutation

    T59/T61A

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-Creb5(T59/T61D)(HA)

    Plasmid

    #195026

    Purpose

    Gateway vector containing HA-tagged Creb5 with T59/T61D mutation

    Depositor

    Cheng-Hai Zhang, Andrew Lassar

    Article

    Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

    Insert

    HA-tagged Creb5 (full length, 508aa; with with T59/T61D mutation) (CREB5 Bovine)
    

    Use

    Gateway cloning

    Tags

    3xHA

    Mutation

    T59/T61D

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDONR221-In18runon-PTPN22

    Plasmid

    #195377

    Purpose

    A human PTPN22 mutant (intron 18-runon) in Gateway pDONR221

    Depositor

    Patrick Concannon

    Article

    Ge et al Diabetes. 2016 Mar;65(3):794-802. doi: 1

    Insert

    PTPN22 (PTPN22 Human)
    

    Use

    Gateway donor vector

    Mutation

    Intron 18-runon PTPN22

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pDONR221 NLRP1 A59P

    Plasmid

    #166816

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation A59P (Herlin et al., 2020) (no stop codon)

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    A59P

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pDONR221 NLRP1 delta787-843

    Plasmid

    #166821

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation _787-843 (Zhong et al., 2016) (no stop codon)

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    _787-843

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pDONR221 NLRP1 T755N

    Plasmid

    #166820

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation T755N (Drutman et al., 2019) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    T755N

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDONR221 NLRP1 R726W

    Plasmid

    #166819

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation R726W (Grandemange et al., 2017) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Mutation

    R726W

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pDONR223 LC3B Q116P G120

    Plasmid

    #129291

    Purpose

    Gateway entry clone encoding human deconjugation-resistant LC3B

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    Changed Glutamine 116 to Proline. Deleted amino a…

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. pDONR223 ATG3 C264A

    Plasmid

    #129293

    Purpose

    Gateway entry clone encoding human ATG3 C264A (catalytic inactive)

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    changed Cysteine 264 to Alanine

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. pDONR223 ATG3 K243R

    Plasmid

    #129294

    Purpose

    Gateway entry clone encoding human ATG3 K243R

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    changed Lysine 243 to Arginine

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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29. pDONR223 ATG3 allKR

    Plasmid

    #129295

    Purpose

    Gateway entry clone encoding human ATG3 with all 22 lysine residues mutated to arginine

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Gateway entry vector / entry clone

    Mutation

    changed all 22 Lysine residues to Arginine

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONR_P4-P1R:PER36modp

    Plasmid

    #128431

    Purpose

    Gateway (Invitrogen) promoter clone (pDONR_P4-P1R) with premature stop codons introduced within the Xero1 and Xero2 genes in the PER36 promoter entry clone (Kunieda et al. 2013).

    Depositor

    George Haughn

    Article

    McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

    Insert

    PEROXISADE36 (AT3G50990 Mustard Weed)
    

    Use

    Gateway promoter entry clone

    Mutation

    Premature stop codons introduced into the Xero1 (…

    Promoter

    PEROXISADE36

    Available Since

    Oct. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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