We narrowed to 12,235 results for: Hal

Showing: 4641 - 4680 of 12235 results

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pColE1_sgRNA_pos6_AmpR

Plasmid

#119149

Purpose

Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 6 in pColE1_70a_deGFP_KanR

Use

Crispr

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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PlayBack-U6-Kpn1

Plasmid

#203297

Purpose

For expression of sgRNAs in a PlayBack Vector with Kpn1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pJW1310

Plasmid

#61253

Purpose

Template for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusion

Depositor

Jordan Ward

Article

Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

Insert

U6 promoter

Use

CRISPR; Cloning template

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA_GFP_hTLN2-FL

Plasmid

#164840

Purpose

Expression of GFP tagged FL human talin2

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN2 (TLN2 Human)

Tags

His10-GFP

Expression

Mammalian

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pUbC-tdTomato

Plasmid

#183710

Purpose

tdTomato expression from UbiC promoter

Depositor

Jeffrey Gerst

Article

Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

Insert

tdTomato

Use

Lentiviral

Promoter

UbiC

Available Since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pRSF-pLtetO-gfp

Plasmid

#89476

Purpose

Broad-host plasmid expressing gfp under pLtetO promoter

Depositor

George Church

Article

Lee et al Nat Microbiol. 2019 Apr 8. pii: 10.1038/

Insert

pLtetO-GFPmut3b

Promoter

pLtetO

Available Since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_NT_AmpR

Plasmid

#119144

Purpose

Encodes non-targeting sgRNA

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

non-targeting sgRNA

Use

Crispr

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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BASIC_SEVA_5a7_pKD46.10

Plasmid

#178937

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available Since

Feb. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pRSetB-Peredox

Plasmid

#73806

Purpose

Expresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in bacteria, with N-terminal his-tag

Depositor

Gary Yellen

Article

Mongeon et al Antioxid Redox Signal. 2016 Mar 18.

Insert

Peredox

Tags

his-tag to facilitate purification

Expression

Bacterial

Promoter

T7

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

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pmyc-MOV10

Plasmid

#10977

Depositor

Thomas Tuschl

Article

Meister et al Curr Biol. 2005 Dec 6;15(23):2149-55. do

Insert

MOV10 (MOV10 Human)

Tags

myc

Expression

Mammalian

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_pos8_AmpR

Plasmid

#119151

Purpose

Encodes sgRNA targeting position 8 in pColE1_70a_deGFP_KanR

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

sgRNA targeting position 8 in pColE1_70a_deGFP_KanR

Use

Crispr

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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APEX2-ChBP

Plasmid

#108878

Purpose

Expression of APEX2 fused to a nanobody against mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-ChBP

Expression

Mammalian

Available Since

May 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAH69

Plasmid

#173782

Purpose

The pAH69 plasmid [Haldimann and Wanner 2001] is the HK022 helper plasmid necessary for genomic integration of the pIT5_KH plasmid.

Depositor

Keith Shearwin

Article

Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102

Insert

HK022 Integrase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJW1662

Plasmid

#112050

Purpose

C-terminal tag with - AID*(AtIAA17_71-114)-HA and OsTIR1 expression cassette marked with LEU

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Inserts

IAA17
TIR1

Tags

HA

Expression

Yeast

Mutation

only amino acids 71-114

Promoter

ADH1 and None

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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AatII-pRPS5A-PTP-3Xflag-16S rRNA Right_G1397N

Plasmid

#189644

Purpose

16S rRNA Right_G1397N

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox

Use

TALEN

Expression

Plant

Promoter

RPS5A

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AatII-pRPS5A-PTP-3Xflag-16S rRNA Right_G1397C_ABE

Plasmid

#189643

Purpose

16S rRNA Right_G1397C_ABE

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox and TadA 8e

Use

TALEN

Expression

Plant

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBPHLWL-VP16AD

Plasmid

#26259

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

VP16AD-ZIP+

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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p3E-APEX2

Plasmid

#108894

Purpose

Multisite gateway vector for 3' tagging with APEX2

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2

Use

Multisite gateway

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry

Plasmid

#108884

Purpose

Multisite gateway vector for 3' tagging with mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mCherry

Use

Multisite gateway

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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lvl0-5-4xFF4

Plasmid

#229170

Purpose

lvl 0 5 prime UTR

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

4xFF4

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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C121-E02: ffLuc2

Plasmid

#162889

Purpose

Standard Gateway entry clone encoding firefly luciferase

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Firefly luciferase2

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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C122-E11: eGFP

Plasmid

#162893

Purpose

Standard Gateway entry clone encoding eGFP fluorescent protein

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

enhanced Green fluorescent protein

Use

Synthetic Biology

Available Since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a-trAtCCD8(pYL735)

Plasmid

#178286

Purpose

Expresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

trAtCCD8

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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PlayBack-U6-Xba1

Plasmid

#203300

Purpose

For expression of sgRNAs in a PlayBack Vector with XbaI as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPd4E-ISH

Plasmid

#61700

Purpose

Plum eIF4E ihp-RNA construct

Depositor

Lining Tian

Article

Wang et al PLoS One. 2013;8(1):e50627. doi: 10.1371

Insert

PdeIF4E

Use

RNAi

Promoter

35S

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
BASIC_SEVA_17_pKD46.10

Plasmid

#178917

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available Since

Feb. 2, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pFETCh_ZBTB1

Plasmid

#128235

Purpose

Donor vector for 3' FLAG tag of human ZBTB1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB1 Homology arms

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pRPS5A-PTP-3Xflag- psa A Left -G1397_C ABE

Plasmid

#189646

Purpose

psa A Left -G1397_C ABE

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox and TadA 8e

Use

TALEN

Expression

Plant

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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PlayBack-Ef1aKpn1

Plasmid

#203307

Purpose

For expression of eukaryotic genes of interest with Ef1a promoter with Kpn1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
EphA1-RTK-Cry2olig

Plasmid

#176601

Purpose

EphrinA1 RTK fusion to Cry2olig - RTK domain only

Depositor

Robert Hughes

Article

Wurz et al bioRxiv [Preprint]. 2024 Feb 7:2024.02.0

Insert

EphA1rtk-Cry2olig-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAWp30

Plasmid

#73857

Purpose

pFUGW-EFSp-Cas9-P2A-Zeo

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

EFSp-Cas9-P2A-Zeo

Use

Lentiviral

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCW-12S-E1A-HA-puro

Plasmid

#212671

Purpose

Express adenovirus E1A

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

12S-E1A

Use

Lentiviral

Tags

HA

Promoter

TRE

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-TagBFP-NS

Plasmid

#75342

Purpose

Multisite gateway entry clone for adding N-terminal fusions of TagBFP

Depositor

Rob Parton

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

TagBFP

Expression

Mammalian

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDH013

Plasmid

#157638

Purpose

sMHT-113 fused to GFP and RFP

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-SYNZIP17-GFP
RFP-SYNZIP18-sMHT(114-23)

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pKI1.1

Plasmid

#85807

Purpose

CRISPR/Cas9 for Arabidopsis in pENTR/D-TOPO

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG and NLS

Promoter

AtRPS5A

Available Since

May 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEQ1007

Plasmid

#83938

Purpose

express human cytomegalovirus IRS1 with a C-terminal 6X his tag in eukaryotic cells

Depositor

Adam Geballe

Article

Hakki et al J Virol. 2006 Dec;80(23):11817-26. Epub

Insert

IRS1

Tags

6xHIS

Expression

Mammalian

Mutation

R833L in IRS1 (please see depositor comment)

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-EGFP-NS

Plasmid

#75341

Purpose

Multisite gateway entry clone for adding N-terminal fusions of EGFP

Depositor

Rob Parton

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

EGFP

Expression

Mammalian

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-mCherry-NS

Plasmid

#108885

Purpose

Multisite gateway vector for 5' tagging with mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mCherry

Use

Multisite gateway

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
AatII-pRPS5A-PTP-3Xflag-rbc L 294 Right_G1397 C-ABE

Plasmid

#189652

Purpose

rbc L 294 Right_G1397 C-ABE

Depositor

Jin-Soo Kim

Article

Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

Insert

TALE and DddAtox and TadA 8e

Use

TALEN

Expression

Plant

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB106-MTS-Citrine

Plasmid

#79135

Purpose

Binary vectors for expression of Citrine fluorescent protein with mitochondria localization signal in plants

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

SD3(1-50)

Tags

Citrine

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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We narrowed to 12,235 results for: Hal