Addgene

We narrowed to 11,043 results for: Hal

Showing: 4641 - 4680 of 11043 results

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pCAG-HArcLight

Plasmid

#138326

Purpose

chemigenetic voltage indicator

Depositor

Eric Schreiter

Article

Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

Insert

HArcLight

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pT3TS-TurboID-EGFP

Plasmid

#163846

Purpose

In vitro transcription of TurboID-EGFP. Parton lab clone JRS

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

TurboID

Use

In vitro transcription of rna

Tags

EGFP

Expression

Mutation

Promoter

Available since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pNB778

Plasmid

#60154

Purpose

Green click beetle luciferase (Promega) driven by LEU1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

CBG99

Use

Tags

Expression

Yeast

Mutation

Promoter

LEU1pr

Available since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

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p3.5VPIII.EGFP.IGR2.1

Plasmid

#40865

Depositor

Harold Gainer

Article

Fields et al J Neurosci. 2003 Aug 27;23(21):7801-9.

Insert

Mouse vasopressin gene (Avp Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

AVP

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hNox3

Plasmid

#58341

Purpose

expresses human Nox3 in mammalian cells

Depositor

Botond Banfi

Article

Kiss et al Curr Biol. 2006 Jan 24;16(2):208-13.

Insert

NADPH oxidase 3 (NOX3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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p3E-csGBP

Plasmid

#108891

Purpose

Multisite gateway vector for 3' tagging with a conditionally stable nanobody against GFP

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

csGBP

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro FLAG-SMAD4 TetON

Plasmid

#107129

Purpose

Inducible expression of Flag-tagged SMAD4 in mammalian cells

Depositor

Joan Massague

Article

David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

Insert

SMAD4

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pB_35S/mEGFP

Plasmid

#135320

Purpose

mEGFP expression regulated by the CaMV35S promoter, co-expressed BASTA resistance selectable marker (bar)

Depositor

Mathias Pribil

Article

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mEGFP

Use

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pME-EGFP-P2A-APEX2-NS

Plasmid

#108895

Purpose

Multisite gateway vector for 5' tagging with APEX2 (includes cytoplasmic GFP reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

EGFP-P2A-APEX2

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

May 19, 2018

Availability

Academic Institutions and Nonprofits only

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pET19b_4CL + STS

Plasmid

#139790

Purpose

expression of biosynthetic enzymes 4CL and STS

Depositor

Roman Jerala

Article

Fink et al AMB Express. 2020 May 24;10(1):97. doi:

Inserts

4CL
STS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pJB01

Plasmid

#111957

Purpose

Expresses His10-RFP-Halo; construct generated for pull-down and localization experiments

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

RFP

Use

Tags

HaloTag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pECNUS_MP01

Plasmid

#194350

Purpose

CRISPR-Cas9 multiplexing acceptor clone with BpiI cutter

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

pcoCas9, FAST-Red

Use

Tags

Expression

Plant

Mutation

Promoter

RPS5A

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Myo 9b

Plasmid

#134907

Purpose

Expression of rat Myo 9b (Myr 5) in mammalian cells.

Depositor

Martin Bähler

Article

Reinhard et al EMBO J. 1995 Feb 15;14(4):697-704.

Insert

Myo 9b (rat) with 3' UTR (Myo9b Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-mVenus

Plasmid

#67719

Purpose

Multisite gateway entry clone for adding C-terminal fusions of mVenus (no ATG)

Depositor

Rob Parton

Article

Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

Insert

mVenus

Use

Gateway multisite 3' entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pMOD_A5803

Plasmid

#176878

Purpose

High constitutive expression of Firefly Luciferase in plants

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

CmYLCV:Luc+:HSP

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-1-AtUbi10-6

Plasmid

#176871

Purpose

MoClo Level 0 plasmid, contains AtUbi1 promoter

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

AtUbi10

Use

Tags

Expression

Plant

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT3TS-miniTurboID-EGFP

Plasmid

#163847

Purpose

In vitro transcription of miniTurboID-EGFP. Parton lab clone JRT

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

miniTurbo

Use

In vitro transcription of rna

Tags

EGFP

Expression

Mutation

Promoter

Available since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMDC32-SEIPIN1

Plasmid

#96973

Purpose

Express Arabidopsis SEIPIN1 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN1 (AT5G16460 Mustard Weed)

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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C413-E15: attB4-EF1p>-attB1r

Plasmid

#162920

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human EF1alpha promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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p416GPD-PYK1

Plasmid

#34604

Depositor

Markus Ralser

Article

Gruning et al Cell Metab. 2011 Sep 7;14(3):415-27.

Insert

PYK1

Use

Tags

Expression

Yeast

Mutation

Promoter

GPD

Available since

Jan. 11, 2012

Availability

Academic Institutions and Nonprofits only

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pMC-1-AtUbi10-6

Plasmid

#197700

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

AtUbi10 promoter

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBPHLWL-GAL4DBD

Plasmid

#26256

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

ZIP- Gal4DBD (GAL4 Budding Yeast)

Use

Drosophila transgenesis

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
OA-1055J

Plasmid

#200251

Purpose

pBac-U6-gRNA(Intersex)-3xp3-tdTomato

Depositor

Omar Akbari

Article

Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

Insert

2 gRNAs targeting Intersex gene

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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3265 pBS Mcl-1

Plasmid

#8763

Depositor

Stanley Korsmeyer

Article

Rinkenberger et al Genes Dev 2000 Jan 1;14(1):23-7.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pGC680

Plasmid

#107018

Purpose

GFP reporter of lag-2 driven by 3 kb promoter

Depositor

Jane Hubbard

Article

Pekar et al Development. 2017 Aug 15;144(16):2896-29

Insert

GFP-PH

Use

Tags

GFP

Expression

Worm

Mutation

Promoter

lag-2

Available since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDR FLIP43 GPS1-NR

Plasmid

#101085

Purpose

GA biosensor expression plasmid

Depositor

Wolf Frommer, Alexander Jones

Article

Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

Insert

GPS1-NR

Use

Tags

6HIS and cMyc

Expression

Yeast

Mutation

AtGAI E51K E54R, AtGID1C S114A F115A

Promoter

pPMA1

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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Clover-Lifeact-7

Plasmid

#54551

Purpose

Localization: Actin, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Use

Tags

Clover

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pDONR-TIR1

Plasmid

#83875

Purpose

Gateway donor vector for TIR1

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

TIR1 (TIR1 Mustard Weed)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS414 GPD-Sis1

Plasmid

#18687

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Sis1 (SIS1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
UBC1cer60G

Plasmid

#161016

Purpose

ubiquitously weakly expressed 60-meric GFP nanocage

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

60meric GFP nanocage

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFETCh_ONECUT2

Plasmid

#135738

Purpose

Donor vector for 3' FLAG tag of human ONECUT2

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ONECUT2 Homology arms (ONECUT2 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
UBC1cer120G

Plasmid

#161017

Purpose

ubiquitously weakly expressed 120-meric GFP nanocage

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

120meric GFP nanocage

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
BCJJ339

Plasmid

#117501

Purpose

RPS5a:Cas9:E9 Golden Gate Level 1 Position 2

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BpiI:GCAA:RPS5a:Cas9-3(intron):E9:ACTA:BpiI

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtRPS5a

Available since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-csChBP

Plasmid

#108893

Purpose

Multisite gateway vector for 3' tagging with a conditionally stable nanobody against mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

dChBP

Use

Multisite gateway

Tags

Expression

Mutation

Promoter

Available since

June 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pN_35S/mEGFP

Plasmid

#132565

Purpose

mEGFP expression regulated by the CaMV35S promoter, co-expressed nourseothricin acetyl transferase selectable marker (nat)

Depositor

Mathias Pribil

Article

Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111

Insert

mEGFP

Use

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEPYCdKN0001

Plasmid

#196148

Purpose

Gateway destination vector for protein expression of 9xHis_AtTGA1_HiBit

Depositor

Nicola Patron

Article

Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

Insert

9xHis_AtTGA1_HiBit

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mg180

Plasmid

#34660

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A2;B0;C1) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Expression

Mutation

Promoter

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pXL-BACII-attPGAL4LWL

Plasmid

#26260

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

GAL4 (GAL4 Budding Yeast)

Use

Drosophila transgenesis

Tags

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pARS3_MUbCAS9_MC

Plasmid

#128767

Purpose

T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a MCS for cloning gRNA

Depositor

Zachary Nimchuk

Article

Worthen et al Development. 2019 Jun 3. pii: dev.174870

Type

Empty backbone

Use

Tags

HA

Expression

Plant

Mutation

Promoter

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p16 GPS1

Plasmid

#101083

Purpose

GA biosensor expression plasmid

Depositor

Wolf Frommer, Alexander Jones

Article

Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

Insert

GPS1

Use

Tags

cMyc

Expression

Plant

Mutation

Promoter

p16 (AT3G60245)

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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