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We narrowed to 8,862 results for: BLI;

Showing: 5801 - 5850 of 8862 results

  1. DN2-Gli3 in pECE

    Plasmid
    #121971
    Purpose
    medium Gli3 transcriptional activator
    Depositor
    James Briscoe
    Insert
    DN2Gli3 (GLI3 Human)
    Use
    Chick electroporation
    Tags
    5xmyc
    Expression
    Mammalian
    Mutation
    N terminus deletion (nt1-1401)
    Promoter
    Available since
    April 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    121971-plasmid-map-sequence-id-241250

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  2. pGEX6P1-DEST-Myc

    Plasmid
    #119752
    Purpose
    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal Myc tag.
    Depositor
    Martin Reijns, Andrew Jackson
    Type
    Empty backbone
    Use
    Tags
    GST and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    Feb. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    119752-plasmid-map-sequence-id-233674

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  3. Reduced Expression Alpha-5-Integrin-GFP

    Plasmid
    #30450
    Depositor
    Rick Horwitz
    Insert
    alpha 5 integrin (ITGA5 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    91-544 of enhancer region of CMV promoter removed…
    Promoter
    Available since
    Aug. 29, 2011
    Availability
    Academic Institutions and Nonprofits only

  4. HAO1

    Plasmid
    #38970
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HAO1 (HAO1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    38970-plasmid-map-sequence-id-471362

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  5. BLVRA

    Plasmid
    #38856
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BLVRA (Blvra Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  6. L57V

    Plasmid
    #34556
    Depositor
    Kevin Truong
    Insert
    L57 (CASP7 Human, A.Sativa (LOV2))
    Use
    Tags
    YFP
    Expression
    Bacterial and Mammalian
    Mutation
    catalytic domain of caspase-7 was truncated at am…
    Promoter
    CMV, T7
    Available since
    April 21, 2012
    Availability
    Academic Institutions and Nonprofits only

  8. ACVRL1

    Plasmid
    #39170
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ACVRL1 (ACVRL1 Human)
    Use
    Baculovirus expression
    Tags
    His Tev
    Expression
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. CLK3

    Plasmid
    #38831
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CLK3 (CLK3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  10. DMPK

    Plasmid
    #38892
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DMPK (DMPK Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  11. pFLAG_G495R∆2CARD

    Plasmid
    #216801
    Purpose
    Expresses a single point mutant of melanoma differentiation-associated protein 5 lacking its 2 CARD domains
    Depositor
    Sun Hur
    Insert
    melanoma differentiation-associated protein 5 delta 2 CARD domains (IFIH1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G495R/∆1-287
    Promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    216801-plasmid-map-sequence-id-433884

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  12. H2AFY2

    Plasmid
    #39052
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    H2AFY2 (MACROH2A2 Human)
    Use
    Tags
    His6-Thioredoxin-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. PTPN14

    Plasmid
    #38944
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPN14 (PTPN14 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. raft-APEX2

    Plasmid
    #205089
    Purpose
    Expresses lipid-raft targeted APEX2 for proximity proteomics. Vector: pcDNA™4/TO. Insert: lipid raft target sequence (MGCVCSS) + mCherry +V5 (GKPIPNPLLGLDST) +APEX2
    Depositor
    Pieta Mattila
    Insert
    APEX2 (raft-mCherry-V5 tagged)
    Use
    Tags
    lipid raft targeting sequence (MGCVCSS), mCherry,…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    205089-plasmid-map-sequence-id-421670

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  16. pCSD_N-SpCas9-1-573-FRB-1xNLS

    Plasmid
    #107298
    Purpose
    Expresses N terminal SpCas9 (1-573 aa) fused to FRB in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    Truncated SpCas9 (aa 1-573) fused to FRB
    Use
    CRISPR
    Tags
    FRB and SV40 NLS
    Expression
    Mammalian
    Mutation
    Truncated SpCas9 (aa 1-573)
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107298-plasmid-map-sequence-id-204614

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  17. pDONR221-DPP4

    Plasmid
    #158439
    Purpose
    Gateway Cloning compatible entry vector for the human DPP4 gene.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    DPP4 (DPP4 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    158439-plasmid-map-sequence-id-308883

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  20. ECH1

    Plasmid
    #38911
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ECH1 (ECH1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  21. LIMK1

    Plasmid
    #39181
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    LIMK1 (LIMK1 Human)
    Use
    Baculovirus expression
    Tags
    His Tev
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    June 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  22. ME1

    Plasmid
    #38857
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ME1 (ME1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  23. K560_AviN_coBirA

    Plasmid
    #129766
    Purpose
    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated on the head for single-molecule assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Use
    Tags
    Avi-tag GLNDIFEAQKIEWH and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Promoter
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129766-plasmid-map-sequence-id-263552

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  24. pTXB1-CI

    Plasmid
    #51565
    Purpose
    Expression of bacteriophage lambda CI for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    cI
    Use
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51565-plasmid-map-sequence-id-78820

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  25. pMSCV-Nras61K-Puro

    Plasmid
    #49404
    Purpose
    MSCVpuro based vector, expressing Nras mutant 61K.
    Depositor
    Tobias Neff
    Insert
    N Ras K61 (NRAS Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    61K constitutively activated
    Promoter
    MSCV promotor
    Available since
    Dec. 9, 2013
    Availability
    Academic Institutions and Nonprofits only

  26. psd44-G13GA

    Plasmid
    #46827
    Purpose
    expression of mutant G protein alpha 13 in mammalian cells
    Depositor
    Agnese Mariotti
    Insert
    GNA13 (GNA13 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    G225A
    Promoter
    PGK promoter
    Available since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only

  27. pET22-6His-SUMO-Nsp10

    Plasmid
    #154960
    Purpose
    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    Use
    Tags
    6His-Thrombin-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    July 21, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154960-plasmid-map-sequence-id-306350

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  28. L57R

    Plasmid
    #34557
    Depositor
    Kevin Truong
    Insert
    L57 (CASP7 Human, A.Sativa (LOV2))
    Use
    Tags
    mRFP
    Expression
    Bacterial and Mammalian
    Mutation
    catalytic domain of caspase-7 was truncated at am…
    Promoter
    CMV, T7
    Available since
    April 24, 2012
    Availability
    Academic Institutions and Nonprofits only

  29. BAZ2B

    Plasmid
    #39000
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BAZ2B (BAZ2B Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  30. pcDNA_DiCre2_59.F2

    Plasmid
    #60209
    Purpose
    Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(19-59)
    Depositor
    Jean-Paul Herman
    Insert
    Cre recombinase (CD207 Synthetic, Human, Bacteriophage P1)
    Use
    Tags
    FKBP12(F36V) and Nuclear localisation signal
    Expression
    Mammalian
    Mutation
    FKBP12 has the F36V mutation; amino acids 19-59 o…
    Promoter
    CMV
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60209-plasmid-map-sequence-id-96295

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  31. CAPZB2-TwinStrepII

    Plasmid
    #83194
    Purpose
    Expression of tagged protein in mammalian cells
    Depositor
    Peter Barr-Gillespie
    Insert
    CAPZB2 (Capzb Mouse)
    Use
    Tags
    Twin Strep Tag II
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    83194-plasmid-map-sequence-id-163493

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  32. pLenti.puro.MCV.ER.RAZ.1

    Plasmid
    #114381
    Purpose
    This lentiviral plasmid expresses MCV tumor T antigens (truncated LT and small T) using the viral NCCR (non-coding control region). The sequence was amplifeied from MCV.R17a and truncated LT=330 aa.
    Depositor
    Sudhir Krishna, Reety Arora
    Insert
    MCV NCCR and tumor T antigen regions
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    MCV NCCR (viral own promoter)
    Available since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    114381-plasmid-map-sequence-id-221609

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  33. GAPDHS

    Plasmid
    #39010
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GAPDHS (GAPDHS Human)
    Use
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  34. pINDUCER20-FLAG-hSRF (#799)

    Plasmid
    #78345
    Purpose
    Doxycycline-inducible lentiviral overexpression of human SRF ORF with a FLAG tag
    Depositor
    Matthew Alexander, Louis Kunkel
    Insert
    SRF (SRF Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mutation
    Promoter
    TRE2
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  35. pcDNA3.1-Beta2-PAmcherry1

    Plasmid
    #114190
    Purpose
    studying clustering of Beta2 receptors by superresolution microscopy (PALM)
    Depositor
    Aleksandra Radenovic
    Insert
    Adrenergic receptor beta 2 (ADRB2 Human)
    Use
    Tags
    3HA, FLAG, and PAmcherry1
    Expression
    Mammalian
    Mutation
    Promoter
    SV40
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    114190-plasmid-map-sequence-id-224309

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  36. 4L59

    Plasmid
    #60087
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    L3MBTL3:JMC057-A08:C223215 (L3MBTL3 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  38. AAV_CWSL.nEF.DIO.Synaptophysin-GCaMP6s.P2A.mRuby3

    Plasmid
    #107736
    Purpose
    In the presence of Cre, can be used to express both GCaMP6s fused to the presynaptic protein synaptophysin and independently the red-fluorescent protein mRuby3.
    Depositor
    Rylan Larsen
    Insert
    synaptophysin-GCaMP6s, mRuby3
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    nEF
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107736-plasmid-map-sequence-id-207278

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  40. isoform PKC Delta (JCE594)

    Plasmid
    #182025
    Purpose
    yeast expression of mouse PKC Delta with yeast PKC1 promoter and ADH1 terminator
    Depositor
    Juan Carlos Igual
    Insert
    PKC Delta (Prkcd Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    yeast PKC1 promoter (-600 to -1)
    Available since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    182025-plasmid-map-sequence-id-361958

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  42. pB6-Hipp11-td-sfGFP dre reporter-FNF-DTA

    Plasmid
    #139513
    Purpose
    B6J Hipp11-pCAG-rox-FNF-bGHpA-3xSV40pA-rox-td-sfGFP-WPRE-bpA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    CAG rox stop rox td-sfGFP dre reporter
    Use
    Mouse Targeting; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    139513-plasmid-map-sequence-id-317907

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  43. ASH1L

    Plasmid
    #39051
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ASH1L (ASH1L Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    39051-plasmid-map-sequence-id-333564

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  44. pMBPcyto-TEV-BseRI

    Plasmid
    #157739
    Purpose
    empty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Use
    Tags
    His10 + MBP (cytoplasmic expression; no signal se…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    157739-plasmid-map-sequence-id-334003

    Enlarge

  45. pJK372

    Plasmid
    #73597
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-15 fatty acid desaturase (FAD3) with C-terminal c-myc epitope
    Depositor
    T. J. Kappock
    Inserts
    delta-12 fatty acid acetylenase
    delta-15 fatty acid desaturase
    Use
    Tags
    FLAG tag and c-myc tag
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73597-plasmid-map-sequence-id-138754

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  46. DAPK3

    Plasmid
    #38964
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DAPK3 (DAPK3 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  47. CD44MMA-c002

    Plasmid
    #175452
    Purpose
    Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, Q23-D172. Used for crystallography.
    Depositor
    Opher Gileadi
    Insert
    Cd44 (Cd44 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175452-plasmid-map-sequence-id-353153

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  48. ALDH7A1

    Plasmid
    #38866
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ALDH7A1 (Aldh7a1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  49. DIRAS2

    Plasmid
    #38819
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DIRAS2 (diras2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  50. 4LG7

    Plasmid
    #60094
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MBD4:JMC02A-C01:C228095 (MBD4 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 5801 - 5850 of 8862 results