We narrowed to 12,549 results for: LIC

1. pTL74

   Plasmid

   #168308

   Purpose

   pGBKT7 yeast two-hybrid plasmid

   Depositor

   Sophien Kamoun

   Article

   Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

   Insert

   Si944233281
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTL73

   Plasmid

   #168307

   Purpose

   pGBKT7 yeast two-hybrid plasmid

   Depositor

   Sophien Kamoun

   Article

   Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

   Insert

   Si944233277
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTL72

   Plasmid

   #168306

   Purpose

   pGBKT7 yeast two-hybrid plasmid

   Depositor

   Sophien Kamoun

   Article

   Bentham et al PLoS Pathog. 2021 Nov 10;17(11):e1009957

   Insert

   Si944231404
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

   Plasmid

   #176573

   Purpose

   Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

   Insert

   Dispersin B
   

   Use

   Tags

   Avitag, Hexahistidine tag, and mEGFP

   Expression

   Bacterial

   Mutation

   E184Q

   Promoter

   T7

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET29b-mVenus-dspB(E184Q W330Y)-6xHis

   Plasmid

   #176577

   Purpose

   Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

   Insert

   Dispersin B
   

   Use

   Tags

   Hexahistidine tag and mVenus

   Expression

   Bacterial

   Mutation

   E184Q W330Y

   Promoter

   T7

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCS2+-wbp2nlY282F

   Plasmid

   #172468

   Purpose

   expresses protein with one aa substitution to affect potential phosphorylation in WW-interaction domain

   Depositor

   Sally Moody

   Article

   Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

   Insert

   wbp2nlY282F
   

   Use

   Xenopus expression

   Tags

   none

   Expression

   Mutation

   changed Tyrosine at aa282 to Phenylalanine

   Promoter

   SP6

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Ecm2 1-197

   Plasmid

   #169924

   Purpose

   WT Ecm2 mutated to introduce stop codon after AA 197 of Ecm2

   Depositor

   Aaron Hoskins

   Article

   van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

   Insert

   Ecm2 1-197
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPPC016.109

   Plasmid

   #171144

   Purpose

   Expression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-GmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Inserts

   J109 scRNA
   J1-BBa_J23117-mRFP
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119 and J1-BBa_J23117

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPPC008.106

   Plasmid

   #171141

   Purpose

   Expression of J106 scRNA on pBBR1-GmR plasmid

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

   Insert

   J106 scRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119

   Available since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2+-wbp2nlF127P

    Plasmid

    #174507

    Purpose

    expresses protein with one aa substitution to disrupt the alpha-helix near the PH-G domain

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nlF127P
    

    Use

    Xenopus expression

    Tags

    none

    Expression

    Mutation

    changed Phenylalanine at aa127 to Proline

    Promoter

    SP6

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2+-wbp2nlT45A

    Plasmid

    #172464

    Purpose

    expresses protein with one aa substitution to affect potential phosphorylation

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nlT45A
    

    Use

    Xenopus expression

    Tags

    none

    Expression

    Mutation

    changed Threonine at aa45 to Alanine

    Promoter

    SP6

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS2+-wbp2nlY55F

    Plasmid

    #172466

    Purpose

    expresses protein with one aa substitution to affect potential phosphorylation in ITSM domain

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nlY55F
    

    Use

    Xenopus expression

    Tags

    none

    Expression

    Mutation

    changed Tyrosine at aa55 to Phenylalanine

    Promoter

    SP6

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBAD-MtrxRFP2

    Plasmid

    #172323

    Purpose

    fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)

    Depositor

    Huiwang Ai

    Article

    Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

    Insert

    human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTopo RE2 Donor pLox hPGK-Puro pA pLox

    Plasmid

    #171049

    Purpose

    Donor template for CRISPR/Cas9 targeting of TERT RE2

    Depositor

    Agnel Sfeir

    Article

    Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

    Insert

    Puromycin
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTopo RE3 Donor pLox hPGK-Puro pA pLox

    Plasmid

    #171050

    Purpose

    Donor template for CRISPR/Cas9 targeting of TERT RE3

    Depositor

    Agnel Sfeir

    Article

    Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

    Insert

    Puromycin
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSUMO chicken-cPLA2-C2-domain

    Plasmid

    #170753

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

    Insert

    PLA2G4A-C2 domain (PLA2G4A Chicken)
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR 221-PHL2 (NON-STOP)

    Plasmid

    #118682

    Purpose

    Gateway entry vector

    Depositor

    Kristiina Himanen

    Article

    Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

    Insert

    PHL2 (AT3G24120 Mustard Weed)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR 221-XERICO (NON-STOP)

    Plasmid

    #118684

    Purpose

    Gateway entry vector

    Depositor

    Kristiina Himanen

    Article

    Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

    Insert

    XERICO (XERICO Mustard Weed)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR-BPC4 (NON-STOP)

    Plasmid

    #118686

    Purpose

    Gateway entry vector

    Depositor

    Kristiina Himanen

    Article

    Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

    Insert

    BPC4 (BPC4 Mustard Weed)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pER8 cHA-XERICO

    Plasmid

    #118690

    Purpose

    Plant inducible expression

    Depositor

    Kristiina Himanen

    Article

    Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

    Insert

    XERICO (XERICO Mustard Weed)
    

    Use

    Tags

    HA

    Expression

    Plant

    Mutation

    Promoter

    LexA

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBA cMYC-PHL2

    Plasmid

    #118669

    Purpose

    Plant overexpression

    Depositor

    Kristiina Himanen

    Article

    Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

    Insert

    PHL2 (AT3G24120 Mustard Weed)
    

    Use

    Tags

    Myc

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDONR 221-AIP2 (NON-STOP)

    Plasmid

    #118678

    Purpose

    Gateway entry vector

    Depositor

    Kristiina Himanen

    Article

    Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

    Insert

    AIP2 (AT5G20910 Mustard Weed)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDONR 221-BPC4 (STOP)

    Plasmid

    #118680

    Purpose

    Gateway entry vector

    Depositor

    Kristiina Himanen

    Article

    Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

    Insert

    BPC4 (BPC4 Mustard Weed)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDONR 221-FG1 (NON-STOP)

    Plasmid

    #118681

    Purpose

    Gateway entry vector

    Depositor

    Kristiina Himanen

    Article

    Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

    Insert

    AT3G07200 (AT3G07200 Mustard Weed)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBA cMYC-AIP2

    Plasmid

    #118667

    Purpose

    Plant overexpression

    Depositor

    Kristiina Himanen

    Article

    Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

    Insert

    AIP2 (AT5G20910 Mustard Weed)
    

    Use

    Tags

    Myc

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJAID5WY

    Plasmid

    #165050

    Purpose

    Integrative expression of SOD1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>SOD1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    OsTIR1 is codon-optimised for expression in S. ce…

    Promoter

    Available since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCW-SPIC

    Plasmid

    #162825

    Purpose

    Doxycycline-inducible overexpression of human SPIC

    Depositor

    Matthew Steinhauser

    Article

    Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

    Insert

    SPIC (SPIC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet ON

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAGM9121_Hsa-Serum Albumin SP

    Plasmid

    #153496

    Purpose

    Human Serum Albumin Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Serum Albumin SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAGM9121_Sce-Killer Protein SP

    Plasmid

    #153498

    Purpose

    S. cerevisiae Killer Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Killer Protein SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAGM9121_Cfo-CPO SP

    Plasmid

    #153500

    Purpose

    Caldariomyces (Leptoxyphium) fumago Chloroperoxidase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Chloroperoxidase SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAGM9121_Cgl-UPO SP

    Plasmid

    #153501

    Purpose

    Chaetomium globosum Unspecific Peroxygenase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Unspecific peroxygenase SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAGM9121_Cci-UPO SP

    Plasmid

    #153502

    Purpose

    Coprinopsis cinerea Unspecific Peroxygenase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Unspecific peroxygenase SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAGM9121_Aae-UPO* SP

    Plasmid

    #153506

    Purpose

    Agrocybe aegerita Unspecific peroxygenase (UPO*) Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Unspecific peroxygenase* SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAGM9121_Mth-UPO SP

    Plasmid

    #153507

    Purpose

    Unspecific peroxygenase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Unspecific peroxygenase SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAGM9121_TEV-His-GFP11

    Plasmid

    #153510

    Purpose

    TEV-His-GFP11 C-terminal Tag Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    TEV-His-GFP11
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAGM9121_Sce-Acid Phosphatase SP

    Plasmid

    #153494

    Purpose

    S. cerevisiae Acid Phosphatase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Acid Phosphatase SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAGM9121_Gma-UPO SP

    Plasmid

    #153495

    Purpose

    Galerina marginata Unspecific Peroxygenase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Unspecific peroxygenase SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJPS2952

    Plasmid

    #135586

    Purpose

    SNR6 has UUC at position 105–107 deleted

    Depositor

    Jonathan Staley

    Article

    Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

    Insert

    snR6 (snR6 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    U6Δ3 has UUC at position 105–107 deleted

    Promoter

    Native

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJPS2953

    Plasmid

    #135587

    Purpose

    SNR6 has AUUUC at position 103–107 deleted

    Depositor

    Jonathan Staley

    Article

    Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

    Insert

    snR6 (snR6 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    U6Δ5 has AUUUC at position 103–107 deleted

    Promoter

    Native

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJPS2955

    Plasmid

    #135589

    Purpose

    SNR6 has UUUAUUUC at position 100–107 deleted

    Depositor

    Jonathan Staley

    Article

    Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

    Insert

    snR6 (snR6 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    U6Δ8 has UUUAUUUC at position 100–107 deleted

    Promoter

    Native

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAGM9121_Mro-UPO SP

    Plasmid

    #153499

    Purpose

    Marasmius rotula Unspecific Peroxygenase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Unspecific peroxygenase SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pACYCDuet1-tglCDEF

    Plasmid

    #134677

    Purpose

    Expresses TglC, TglD, TglE, TglF in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Inserts

    TglC
    TglD
    TglE
    TglF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pET28b-GFP-tglG

    Plasmid

    #134679

    Purpose

    Expresses His6-GFP-TglG fusion in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Insert

    TglG
    

    Use

    Tags

    His6-GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pET15b-tglHI

    Plasmid

    #134672

    Purpose

    Expresses His6-TglH and TglI in E. Coli

    Depositor

    Wilfred van der Donk

    Article

    Ting et al Science. 2019 Jul 19;365(6450):280-284.

    Inserts

    TglH
    TglI
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pGBW-m4046911

    Plasmid

    #145695

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 helicase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

46. pGBW-m4046851

    Plasmid

    #145614

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 helicase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

47. pGBW-m4046937

    Plasmid

    #145609

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 helicase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

48. pCDNA3.0_LynNanobody IRES EGFP

    Plasmid

    #136624

    Purpose

    Plasmid encoding outer inner membrane localization of the Enhancer nanobody GFP as a SNAP-Tag-fusion and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    Lyn11-SNAP-Tag-EnhancerNb (IRES EGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pYPQ290-D977A

    Plasmid

    #129674

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AacCas12b
    

    Use

    CRISPR; Gateway compatible aaccas12b entry clone

    Tags

    Expression

    Plant

    Mutation

    D977A; AacCas12b is rice codon optimized

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pYPQ290-E848A

    Plasmid

    #129675

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AacCas12b
    

    Use

    CRISPR; Gateway compatible aaccas12b entry clone

    Tags

    E848A; AacCas12b is rice codon optimized

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge