We narrowed to 3,053 results for: PPL;

1. pME-rox-nls-mCherry-V5-2xStop-rox (JDW 1167)

   Plasmid

   #224524

   Purpose

   A Gateway compatible middle entry clone containing a Rox flanked 3xNLS-mCherry-V5-2xStop cassette

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   rox-3xNLS-mCherry-V5-2xSTOP-rox
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p5E-EFS (EF1a core) (JDW 1319)

   Plasmid

   #224474

   Purpose

   Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   EFS promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)

   Plasmid

   #224505

   Purpose

   Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expression

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LexA-mODC-LexOP-35SCaMV
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-LexA-mODC-LexOP-c-Fos (JDW 1359)

   Plasmid

   #224506

   Purpose

   Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with 3' minimal c-Fos promoter for an all in one RU486 driven gene expression construct

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LexA-oMDC-LexOP-c-Fos
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME TAEL2.0-5xC120-minpro (JDW 1109)

   Plasmid

   #224507

   Purpose

   Gateway compatible middle entry clone containing TAEL2.0 transcription factor and 5xC120 response element (All in one binary TAEL and effector domain clone)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   TAEL2.0-5xC120
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

   Plasmid

   #224512

   Purpose

   A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   3xHA-BirA-T2A-mCherry-Caax
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)

   Plasmid

   #224522

   Purpose

   A Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassette

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC1_oROS-HT_LF(C199S)

   Plasmid

   #216412

   Purpose

   Expresses the loss-of-function mutation C199S of the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   oROS-HT_LF(C199S)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC1-lifeact-oROS-HT

   Plasmid

   #216420

   Purpose

   Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to actin filaments.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   lifeact-oROS-HT
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. BII-ChBtW-mADAR2-short

    Plasmid

    #175588

    Purpose

    mammalian expression of mouse short Adar2, blasticidine selection

    Depositor

    Emanuela Felley-Bosco

    Article

    Hariharan et al Mol Oncol. 2022 Dec;16(22):3949-3974. do

    Insert

    Short Mouse Adar2
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pS97splitRBP_S2-Enz

    Plasmid

    #183768

    Purpose

    Expresses SHIP2-Enzyme domain flanked by RBP

    Depositor

    William Kerr

    Article

    Sudan et al Front Immunol. 2022 May 4;13:830961. doi

    Insert

    SHIP2 (INPPL1 Human)
    

    Use

    Tags

    6HIS, RBP (C-terminal) HRV3C cleavage site, and R…

    Expression

    Bacterial

    Mutation

    includes aa K414 to aa V872 only

    Promoter

    T7lac

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmirGlo-RBM8A-3'UTR AluJo-Y 240-2996

    Plasmid

    #171131

    Purpose

    reporter

    Depositor

    Emanuela Felley-Bosco

    Article

    Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

    Insert

    RBM8A 3'UTR (RBM8A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmirGlo-RBM8A-3'UTR AluSz 3501-4065

    Plasmid

    #171132

    Purpose

    reporter

    Depositor

    Emanuela Felley-Bosco

    Article

    Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

    Insert

    RBM8A 3'UTR (RBM8A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pmirGlo-RBM8A-3'UTR AluY WT 2598-2996

    Plasmid

    #171134

    Purpose

    reporter

    Depositor

    Emanuela Felley-Bosco

    Article

    Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

    Insert

    RBM8A 3'UTR (RBM8A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pmirGlo-RBM8A-3'UTR AluY-Sz WT 2598-4066

    Plasmid

    #171136

    Purpose

    reporter

    Depositor

    Emanuela Felley-Bosco

    Article

    Abukar et al Cells. 2021 Dec 15;10(12). pii: cells101

    Insert

    RBM8A 3'UTR (RBM8A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. DLG3_PDZ_2

    Plasmid

    #103947

    Purpose

    Protein expression and purification of NE-DLG PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    DLG3 (DLG3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. MAGI1_PDZ_1

    Plasmid

    #103898

    Purpose

    Protein expression and purification of MAGI1 PDZ1 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    MAGI1 (MAGI1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MAGI1_PDZ_4

    Plasmid

    #103901

    Purpose

    Protein expression and purification of MAGI1 PDZ4 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    MAGI1 (MAGI1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG PSAM4-5HT3 HC IRES EGFP

    Plasmid

    #119740

    Purpose

    Chemogenetic activator expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Insert

    PSAM4 a75HT3 HC (homo) IRES eGFP (CHRNA7, HTR3A Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F, R420Q, R424D, R428A

    Promoter

    CAG

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMIG-FLAG-MLL-AF9

    Plasmid

    #71443

    Purpose

    Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-GFP

    Depositor

    Daisuke Nakada

    Article

    Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

    Insert

    MLL-Af9 (KMT2A Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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