We narrowed to 3,255 results for: actin

1. 413-Met25 Kar2 TorsinADE K108A HDEL

   Plasmid

   #32122

   Depositor

   Susan Lindquist

   Article

   Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

   Insert

   TorsinA (TOR1A Human)
   

   Tags

   HDEL and Kar2

   Expression

   Yeast

   Mutation

   deletion of E at 302 or 303, K108A

   Available Since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pFU-GW-sMYH6-mNeon-TNNT2-mScarlet

   Plasmid

   #170712

   Purpose

   dual fluorescent reporter for MYH6 (short) and TNNT2 promoters

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Inserts

   mNeonGreen
   mScarlet
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TNNT2 and aMHC

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIZ-Flag6His-BmAgo3

   Plasmid

   #50559

   Purpose

   Expresses FLAG, His tagged BmAgo3 in silkworm cell line BmN4 or insect cells

   Depositor

   Yukihide Tomari

   Article

   Kawaoka et al RNA. 2009 Jul;15(7):1258-64. doi: 10.126

   Insert

   BmAgo3
   

   Tags

   6xHIS and Flag

   Expression

   Insect

   Promoter

   OpIE2

   Available Since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFlag-CMV2-Brd4 del ET

   Plasmid

   #21938

   Depositor

   Eric Verdin

   Article

   Bisgrove et al Proc Natl Acad Sci U S A. 2007 Aug 21. 1

   Insert

   BRD4 (BRD4 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   aa 1-1362 only, ET domain deleted

   Available Since

   Sept. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

5. EGFP-IC2-FL

   Plasmid

   #51409

   Purpose

   expression of EGFP-tagged full-length dynein intermediate chain IC2C in mammalian cells

   Depositor

   Trina Schroer

   Article

   King et al Mol Biol Cell. 2003 Dec;14(12):5089-97.

   Insert

   dynein intermediate chain IC2C (Dync1i2 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDS240

   Plasmid

   #34966

   Purpose

   Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope).

   Depositor

   Michael Glotzer

   Article

   Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

   Insert

   Mid2(SS/TM)-GFP-LOVpep
   

   Expression

   Yeast

   Mutation

   K533∆, high-affinity epitope

   Promoter

   GAL1

   Available Since

   March 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRT498-OtUBD

   Plasmid

   #190089

   Purpose

   Expresses a single OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

   Depositor

   Mark Hochstrasser

   Article

   Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

   Insert

   OtUBD
   

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGM1202

   Plasmid

   #69615

   Purpose

   inducible gene expression in Streptomyces, C-terminal His-tag encoding sequence

   Depositor

   Günther Muth

   Article

   Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

   Inserts

   aac
   tsr
   rep
   sso
   TipA promoter
   dso
   

   Available Since

   Jan. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNIFV

   Plasmid

   #111085

   Purpose

   pNIFV is a vector plasmid with mutations in the cis-acting region, to prevent correction of the integrase by recombination when used with pCiPS-NIFV to produce Non-Integrating Foamy Virus vectors

   Depositor

   David Russell

   Article

   Deyle et al J Virol. 2010 Sep;84(18):9341-9. doi: 10

   Insert

   empty backbone/deleted foamy vector, same as pΔΦ with mutations in cis-acting pol region
   

   Use

   Deleted foamy virus

   Mutation

   at nt 2334 A changed to T, at nt 2495 A changed t…

   Promoter

   CMV

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21a-cys-His6-OtUBD

    Plasmid

    #190091

    Purpose

    Expresses OtUBD with a non-cleavable N-terminal 6xHis tag as well as an engineered N-terminal cysteine in E. coli

    Depositor

    Mark Hochstrasser

    Article

    Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

    Insert

    OtUBD
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-GFP-Flag-UTR

    Plasmid

    #183235

    Purpose

    To express FLAG tagged eGFP

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    EGFP
    

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV4-NIK-HA

    Plasmid

    #27554

    Depositor

    Shao-Cong Sun

    Article

    Xiao et al J Biol Chem. 2000 Jul 14. 275(28):21081-

    Insert

    NIK (MAP3K14 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    NIK starts at amino acid 3; C52R and R139H mutati…

    Promoter

    CMV

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

13. linker-mCitrine-PIF6-NAT

    Plasmid

    #51576

    Purpose

    Tag protein of interest with PIF6 in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PIF6 (PIL2 Mustard Weed)
    

    Tags

    linker and mCitrine

    Expression

    Yeast

    Mutation

    1-100aa of PIF6

    Promoter

    none

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3 FLAG_CMV14/OCTN2 (wt)

    Plasmid

    #112799

    Purpose

    Expression of OCTN2

    Depositor

    Katarzyna Nalęcz

    Article

    Czeredys et al PLoS One. 2013 Dec 13;8(12):e82105. doi:

    Insert

    OCTN2/SLC22A5 (Slc22a5 Rat)
    

    Tags

    3 x Flag

    Expression

    Mammalian

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRX-IPU-FLAG-NEK9

    Plasmid

    #168269

    Purpose

    Expresses NEK9 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

    Insert

    NEK9 (NEK9 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. TAK11

    Plasmid

    #227095

    Purpose

    Luciferase reporter vector with HIV-1 TAR region cloned in pGL3 Basic vector

    Depositor

    Farah Mustafa

    Article

    Khader et al Commun Biol. 2024 Nov 16;7(1):1521. doi:

    Insert

    CMV Promoter and HIV-1 TAR region
    

    Use

    Luciferase

    Promoter

    CMV

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAL175

    Plasmid

    #22276

    Purpose

    Expresses YFP-PIF for red-light-induced binding to Phy

    Depositor

    Christopher Voigt

    Article

    Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

    Insert

    PIF6APB (PIL2 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    mYFP

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRT498-3xOtUBD

    Plasmid

    #190088

    Purpose

    Expresses 3 tandem OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

    Depositor

    Mark Hochstrasser

    Article

    Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

    Insert

    3xOtUBD
    

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDS275

    Plasmid

    #34972

    Purpose

    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(T406A, T407A, I532A mutations).

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Expression

    Yeast

    Mutation

    T406A, T407A, I532A

    Available Since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJS183

    Plasmid

    #134142

    Purpose

    Bacterial expression of GFP-Ska1 and Ska2

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-GFP-human Ska1; human Ska2
    

    Tags

    GFP

    Expression

    Bacterial

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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