We narrowed to 11,959 results for: pcDNA

1. pcdna3 flag - ULK2 wt

   Plasmid

   #27637

   Depositor

   Reuben Shaw

   Article

   Egan et al Science. 2011 Jan 28;331(6016):456-61. d

   Insert

   mouse ULK2 (Ulk2 Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Initial M removed from ULK2.

   Promoter

   Available since

   March 16, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA5-FRT-TO-eGFP

   Plasmid

   #157733

   Purpose

   mammalian expression of untagged eGFP under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA5-FRT-TO-Nsp13_GC3opt

   Plasmid

   #157714

   Purpose

   mammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pCDNA3-HA-Akt1-K179M

   Plasmid

   #73409

   Purpose

   Expresses kinase-inactive mutant of Akt with HA tag

   Depositor

   Jie Chen

   Article

   Zeng et al Mol Biol Cell. 2014 Dec 1;25(24):3954-61

   Insert

   AKT1 (AKT1 Human)
   

   Use

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   changed K179M to make Kinase inactive mutant

   Promoter

   CMV

   Available since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO V5 HSPA6

   Plasmid

   #19513

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   heat shock 70kDa protein 6 (HSPA6 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3puro-mGFP-TDRD3

   Plasmid

   #225469

   Purpose

   Express mEGFP-fusion protein of TDRD3

   Depositor

   Christine Mayr

   Article

   Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

   Insert

   TDRD3 (TDRD3 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA4/TO/GFP-GIGYF1

   Plasmid

   #141188

   Purpose

   Expresses GFP-GIGYF1 in mammalian cells, can be used to make inducible cell line

   Depositor

   Simon Bekker-Jensen

   Article

   Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

   Insert

   GIGYF1 (GIGYF1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3 c-SRC (1-397)

   Plasmid

   #42205

   Depositor

   Robert Lefkowitz

   Article

   Miller et al J Biol Chem. 2000 Apr 14;275(15):11312-9

   Insert

   c-SRC (1-397) (SRC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   contains amino acids 1-397

   Promoter

   CMV

   Available since

   Feb. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1/BCR-FGFR1

   Plasmid

   #167490

   Purpose

   Mammalian expression vector for the BCR-FGFR1 fusion gene associated with myeloid and lymphoid neoplasms

   Depositor

   Nicholas Cross

   Article

   Demiroglu et al Blood. 2001 Dec 15;98(13):3778-83. doi:

   Insert

   BCR-FGFR1 (BCR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA5/mCherry_pBirA-HA_FKBP8

    Plasmid

    #154457

    Purpose

    expresses mCherry_pBirA-HA_FKBP8/biochemical assay

    Depositor

    Hyun-Woo Rhee

    Article

    Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

    Insert

    mCherry_pBirA-HA_FKBP8 (FKBP8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-F

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1/HisA/Serca1

    Plasmid

    #74402

    Purpose

    expression of SERCA in mammalian cells

    Depositor

    Robert Nakamoto

    Insert

    SERCA1 (ATP2A1 rabbit)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    NcoI site destroyed (silent) T898

    Promoter

    CMV

    Available since

    April 6, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pCDNA3-AUP1-FLAG 5E

    Plasmid

    #185341

    Purpose

    Assessing G2BR function in AUP1

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    AUP1 (AUP1 Human)
    

    Use

    Tags

    Flag, 6xHis

    Expression

    Mammalian

    Mutation

    R382E/Q383E/K390E/R398E/R400E

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-nuc-Nb80

    Plasmid

    #205519

    Purpose

    Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    nuc-Nb80
    

    Use

    Tags

    Nuclear localization signal (NLS) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. BACE2_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176942

    Purpose

    Mammalian expression vector encoding BACE2 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    BACE2 (BACE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LacZ-5BoxB in pcDNA3.1+

    Plasmid

    #29729

    Depositor

    Howard Chang

    Article

    Wang et al Nature. 2011 Apr 7;472(7341):120-4. doi:

    Insert

    LacZ-5BoxB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3.1/VChR1-EYFP

    Plasmid

    #20955

    Purpose

    Mammalian expression of VChR1 fused to EYFP, for neuronal excitation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nat Neurosci. 2008 Jun . 11(6):631-3.

    Insert

    volvox channelrhodopsin-1
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-tdMCP-24xGCN4

    Plasmid

    #205157

    Purpose

    express tdMCP-24xGCN4

    Depositor

    Weirui Ma

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    tdMCP-24xGCN4
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-MS2-HA-HsDIS3_Z

    Plasmid

    #148111

    Purpose

    Mammalian Expression of HsDIS3

    Depositor

    Elisa Izaurralde

    Insert

    HsDIS3 (DIS3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L760F compared to the sequence given by isoform 2…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-CaMPARI V398D

    Plasmid

    #60786

    Purpose

    Expresses CaMPARI V398D in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

    Depositor

    Eric Schreiter, Loren Looger

    Article

    Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

    Insert

    CaMPARI V398D
    

    Use

    Tags

    Nuclear Export Signal

    Expression

    Mammalian

    Mutation

    V398D

    Promoter

    CMV

    Available since

    Nov. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-HA-Nedd4/2795

    Plasmid

    #11426

    Depositor

    Allan Weissman

    Article

    Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

    Insert

    Nedd4-1 (Nedd4 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D661N compared to GenBank reference sequence U966…

    Promoter

    CMV

    Available since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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