We narrowed to 9,006 results for: Pol;

1. D-raf R217L+C295S

   Plasmid

   #39764

   Depositor

   Norbert Perrimon

   Article

   Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

   Insert

   d-raf (Raf Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   R217L and C295S

   Promoter

   Available since

   Jan. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pCRISPRi_Mxi1_yl

   Plasmid

   #91248

   Purpose

   CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

   Inserts

   Codon optimized dCas9-Mxi1
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-28a_6H-TAQ_E602D

   Plasmid

   #166944

   Purpose

   Bacterial expression of Taq DNA polymerase

   Depositor

   Robert Tjian

   Article

   Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

   Insert

   Thermus aquaticus DNA polymerase
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   E602D

   Promoter

   T7

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. PRE-Hsp70BbCas9_1.3

   Plasmid

   #190797

   Purpose

   aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product

   Depositor

   Omar Akbari

   Article

   Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

   Insert

   2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB_TRE3G_scFV-sfGFP-Kmt5cFL

   Plasmid

   #235581

   Purpose

   Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Kmt5c fused with scFV to programme H4K20me3

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   Kmt5c (Kmt5c Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCCM’

   Plasmid

   #162709

   Purpose

   pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   Second CCM operon cloned from H. neapolitanus
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   p15A origin replaced with high-copy colE1 origin

   Promoter

   PLtet0-1 promoter

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEAK-Flag/INPP4B

   Plasmid

   #24324

   Depositor

   Lewis Cantley

   Article

   Gewinner et al Cancer Cell. 2009 Aug 4. 16(2):115-25.

   Insert

   Inositol Polyphosphate 4-Phosphatase Type II (INPP4B Human)
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available since

   May 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. eDrIR1

   Plasmid

   #183998

   Purpose

   Optically controlled eDrIR1 expression in mammalian cells

   Depositor

   Vladislav Verkhusha, Dmitry Gerashchenko

   Article

   Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

   Insert

   eDrIR1 (INSR Human)
   

   Use

   Tags

   Ig kappa secretory signal, mCherry, Deinococcus r…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Syn-VARNAM2-Kv2.1PR

   Plasmid

   #195524

   Purpose

   Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

   Insert

   VARNAM2-Kv2.1 proximal restriction sequence
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   VARNAM SI linker

   Promoter

   Synapsin

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB_TRE3G_scFV-sfGFP-Kmt5cFL-CatMut

    Plasmid

    #235590

    Purpose

    Dox-inducible expression of control catalytic-mutant Kmt5c CD fused with scFV

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Kmt5c (Kmt5c Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    NHDC182-185AAAG

    Promoter

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid

    #91249

    Purpose

    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Syn-Ace-mNeon2

    Plasmid

    #195518

    Purpose

    Green fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    Ace-mNeon2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Ace-mNeon 81S; SY linker

    Promoter

    Synapsin

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF1α:flex-mcherry

    Plasmid

    #222618

    Purpose

    Cre-dependent expression of the red fluorescent protein mCherry under control of the EF1a promoter

    Depositor

    Franck Polleux

    Article

    Blockus et al Cell Rep. 2021 Oct 19;37(3):109828. doi:

    Insert

    Cre-dependent mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG mEmerald-LAMP1

    Plasmid

    #168513

    Purpose

    Expression of rat LAMP1 fused with mEmerald to perform live tracking of lysosomes

    Depositor

    Franck Polleux

    Article

    Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CaMKII-Ace-mNeon2

    Plasmid

    #195526

    Purpose

    Green fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    Ace-mNeon2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Ace-mNeon 81S; SY linker

    Promoter

    CaMKII

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRcCMV myc-Plk1 wt (Nigg RG6)

    Plasmid

    #41160

    Depositor

    Erich Nigg

    Article

    Golsteyn et al J Cell Sci. 1994 Jun;107 ( Pt 6):1509-17

    Insert

    Plk1 (PLK1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CAG-fDIO-VARNAM2-Kv2.1PR

    Plasmid

    #195536

    Purpose

    Flp recombinase-dependent red fluorescent, negative response-polarity voltage indicator; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    VARNAM2-Kv2.1 proximal restriction sequence
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    VARNAM SI linker

    Promoter

    CAG

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. p3xFLAG-CMV-Tlr4

    Plasmid

    #27148

    Depositor

    Bruce Beutler

    Article

    Poltorak et al Science. 1998 Dec 11. 282(5396):2085-8.

    Insert

    toll-like receptor 4 (Tlr4 Mouse)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Native signal peptide sequence (original amino ac…

    Promoter

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-CaMKII-VARNAM2-Kv2.1PR

    Plasmid

    #195530

    Purpose

    Red fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    VARNAM2-Kv2.1 proximal restriction sequence
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    VARNAM SI linker

    Promoter

    CaMKII

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNOC-CRISPR-P2A-BlastR

    Plasmid

    #98147

    Purpose

    Expresses Cas9-Nlux-P2A-BlastR and sgRNA in Nannochloropsis oceanica.

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    P2A-BlastR fusion with Cas9-Nlux

    Expression

    Mutation

    Promoter

    Ribi promoter

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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