We narrowed to 3,747 results for: mak

1. pDEST-DHFR F[1,2]-C (TRP1)

   Plasmid

   #177795

   Purpose

   Gateway destination vector to insert genes of interest having a C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

   Type

   Empty backbone

   Tags

   DHFR F[1,2]

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn Con/Fon EYFP

   Plasmid

   #55650

   Purpose

   Cre-on/Flp-on EYFP under the Synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV8

   Insert

   eYFP with introns
   

   Use

   AAV; Cre on/flp on eyfp

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Ef1a-DIO hChR2(C128S/D156A)-EYFP

   Plasmid

   #35503

   Purpose

   AAV expression of Ef1a-driven, cre-dependent, stabilized step function opsin (SSFO) for optogenetic activation.

   Depositor

   Karl Deisseroth

   Article

   Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

   Insert

   hChR2(C128S/D156A)-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   C128S and D156A

   Promoter

   Ef1a

   Available Since

   April 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-vCreDIO hChR2(H134R)-EYFP

   Plasmid

   #55643

   Purpose

   vCre-Dependent ChR2-EYFP

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   hChR2(H134R)-EYFP
   

   Use

   AAV; Vcre-dependent chr2-eyfp

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Aug. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHE111-3xFlag-HSP70

   Plasmid

   #236055

   Purpose

   Mammalian expression of human HSP70 with a N-terminal 3x-FLAG tag

   Depositor

   Jonathan Schisler

   Article

   Stewart et al bioRxiv 2025.02.17.637997

   Insert

   HSP70 (HSPA1A Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   Aug. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-AmCyan

   Plasmid

   #138481

   Purpose

   For cloning sgRNA flanked by two ribozyme elements, to subsequently cloned into PL-5LTR-GW-A vector.

   Depositor

   Akitsu Hotta

   Article

   Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

   Insert

   AmCyan
   

   Expression

   Mammalian

   Available Since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MBP-FUS_LC_WT

   Plasmid

   #98653

   Purpose

   Expresses MBP-tagged FUS AA 1-163

   Depositor

   Nicolas Fawzi

   Article

   Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

   Insert

   FUS (FUS Human)
   

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Available Since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO-Tet-On-ARNTL-shRNA1

   Plasmid

   #166913

   Purpose

   Lentivirus for inducible knockdown of human ARNTL(BMAL1)

   Depositor

   Roland Friedel

   Article

   Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

   Insert

   ARNTL shRNA (BMAL1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-nEF-ChRmine-mScarlet

   Plasmid

   #137158

   Purpose

   Viral expression of ChRmine-p2a-mScarlet

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   ChRmine-p2a-mScarlet
   

   Use

   AAV

   Mutation

   n/a

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. WT Smarca4-V5 IRES-puro

    Plasmid

    #107058

    Purpose

    Expression of wild-type Smarca4 (Brg1)-V5 fusion with IRES puromycin resistance.

    Depositor

    Jerry Crabtree, Courtney Hodges

    Article

    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    

    Use

    Lentiviral

    Tags

    IRES-puroR and V5

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CAG

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-HA-FbxO5-pcDNA3.1-

    Plasmid

    #52509

    Purpose

    expresses human FbxO5 in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    FbxO5 (FBXO5 Human, FbxO5)
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. mIFP-TOMM20-N-10

    Plasmid

    #56239

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    NM_014765.2 (TOMM20 Human)
    

    Tags

    mIFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. MBP-FUS_FL_12E

    Plasmid

    #98652

    Purpose

    Expresses MBP-tagged full length phosphomimetic FUS

    Depositor

    Nicolas Fawzi

    Article

    Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

    Insert

    FUS (FUS Human)
    

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    12 S/T to E

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas-dCas12m

    Plasmid

    #192275

    Purpose

    Encodes MmdCas12m (D485A) under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmdCas12m
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D485A

    Promoter

    PJ23108

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Luciferase

    Plasmid

    #213979

    Purpose

    Expresses Doxy Inducible Luciferase

    Depositor

    Coleman Lindsley

    Article

    Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    6xHis affinity tag

    Expression

    Bacterial and Mammalian

    Promoter

    Tet-responsive promoter PTight, consisting of sev…

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pInt-ERCreER

    Plasmid

    #190040

    Purpose

    This is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).

    Depositor

    Kayoko Tanaka

    Article

    Hindul et al Biol Open. 2022 Feb 15;11(2). pii: 27408

    Insert

    ERT2-Cre-ERT2 flanked by AAVS1
    

    Use

    CRISPR and TALEN

    Expression

    Mammalian

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. 8xNFAT-ZsG-muhCD4

    Plasmid

    #162745

    Purpose

    NFAT-driven ZsGreen-1 reporter gene with mutated human CD4 gene

    Depositor

    Maki Nakayama

    Article

    Landry et al Front Endocrinol (Lausanne). 2021 Mar 25

    Insert

    8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (CD4 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Human CD4 gene with glutamine to tyrosine at posi…

    Available Since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas-Cas12m

    Plasmid

    #192274

    Purpose

    Encodes MmCas12m under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmCas12
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PJ23108

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO-Tet-On-ARNTL-shRNA2

    Plasmid

    #166914

    Purpose

    Lentivirus for inducible knockdown of human ARNTL(BMAL1)

    Depositor

    Roland Friedel

    Article

    Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

    Insert

    ARNTL shRNA (BMAL1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry

    Plasmid

    #35498

    Purpose

    AAV expression of Ef1a-driven, cre-dependent, chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    ChR1-VChR1 Chimera
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E122T and E162T

    Promoter

    Ef1a

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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