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hnRNPA2_LC_NtoS

Plasmid

#139121

Purpose

express hnRNPA2 with all N changed to S

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

all N to S

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_5E

Plasmid

#139122

Purpose

express hnRNPA2 with 5 serine changed to glutamate

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

5 serine to glutamate

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPA2_LC_12E

Plasmid

#139123

Purpose

express hnRNPA2 with all serine change to glutamate

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

12 S to E

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPF_PLD_YtoS

Plasmid

#139124

Purpose

express hnRNPF PLD with all Y changed to S

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

all Y to S

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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hnRNPF_PLD_StoA

Plasmid

#139125

Purpose

express hnRNPF PLD with all S changed to A

Depositor

Nicolas Fawzi

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

all S to A

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1045B

Plasmid

#125004

Purpose

Expresses gRNAs targeting hid, eve, and hedgehog

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

eve gRNA (eve Fly)

Expression

Insect

Promoter

U6:3

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1045D

Plasmid

#125006

Purpose

Expresses gRNAs targeting hedgehog and wingless

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

wg gRNA (wg Fly)

Expression

Insect

Promoter

u6:3

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1045E

Plasmid

#125007

Purpose

Expresses tRNA-flanked gRNAs targeting hid, eve, and hedgehog

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

eve gRNA (eve Fly)

Expression

Insect

Promoter

U6:3

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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OA-1045A

Plasmid

#125003

Purpose

Expresses gRNAs targeting hid and eve

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

eve gRNA (eve Fly)

Expression

Insect

Promoter

U6:3

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132) K97E

Plasmid

#125825

Purpose

Expresses GST-RPN13 (19-132) K97E in E.coli

Depositor

Chris Hill

Article

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

changed lysine 97 to glutamate

Promoter

T7

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132) K83A

Plasmid

#125829

Purpose

Expresses GST-RPN13 (19-132) K83A in E.coli

Depositor

Chris Hill

Article

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

changed lysine 83 to alanine

Promoter

T7

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132) K97A

Plasmid

#125823

Purpose

Expresses GST-RPN13 (19-132) K97A in E.coli

Depositor

Chris Hill

Article

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

changed lysine 97 to alanine

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET151-GST-PP-RPN13 (19-132) K97D

Plasmid

#125824

Purpose

Expresses GST-RPN13 (19-132) K97D in E.coli

Depositor

Chris Hill

Article

Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

Insert

RPN13 (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

changed lysine 97 to aspartate

Promoter

T7

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pUCHR-CD5HA2-P2A-smAID-mClover

Plasmid

#110658

Purpose

Lentiviral vector expressing cell surface HA-tag and monomeric GFP N-terminaly fused with auxin inducible degron

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

CD5HA2-P2A-AID-mClover

Use

Lentiviral

Promoter

CMV

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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hHSF4b K294R

Plasmid

#118357

Purpose

This plasmid expresses human HSF4b K294R mutant, which cannot undergo phosphorylation-dependent sumoylation.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hHSF4b (HSF4 Human)

Expression

Mammalian

Mutation

K294R

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-Fos S362DS374A

Plasmid

#118305

Purpose

Expression of mous C-Fos

Depositor

Philip Stork

Article

Pellegrino et al J Neurochem. 2006 Dec;99(6):1480-93. doi

Insert

C-Fos S362DS374A (Fos Mouse)

Tags

Flag

Expression

Mammalian

Mutation

S362DS374A

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-Fos S362AS374D

Plasmid

#118304

Purpose

Expression of mouse C-Fos

Depositor

Philip Stork

Article

Pellegrino et al J Neurochem. 2006 Dec;99(6):1480-93. doi

Insert

C-Fos S362AS374D (Fos Mouse)

Tags

Flag

Expression

Mammalian

Mutation

S362AS374D

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-Fos S362D

Plasmid

#118303

Purpose

Expression of mouse C-Fos

Depositor

Philip Stork

Article

Pellegrino et al J Neurochem. 2006 Dec;99(6):1480-93. doi

Insert

C-Fos S362D (Fos Mouse)

Tags

Flag

Expression

Mammalian

Mutation

S362D

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-shLARP7

Plasmid

#113537

Purpose

Retroviral vector designed to knock down LARP7.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shLARP7 (LARP7 Human)

Use

Retroviral

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1-MePCE_aa470-689

Plasmid

#113544

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 470 - 689.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1_MePCE_aa432-689

Plasmid

#113543

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 432 - 689.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1_MePCE_aa400-689

Plasmid

#113542

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 400 - 689.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1_MePCE_aa1-400

Plasmid

#113541

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 1 - 400.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3H09

Plasmid

#71003

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

SNF4Agamma (SNF4Agamma Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3C07

Plasmid

#70985

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

14-3-3zeta (14-3-3zeta Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2B09

Plasmid

#70918

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

gro (gro Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3C03

Plasmid

#70959

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf4 (Taf4 Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1-Shp2 T7DA/S189D SH2s

Plasmid

#67577

Purpose

Expresses GST-tagged Shp2 T73D/S189D SH2 domains in bacteria

Depositor

Graeme Carnegie

Article

Burmeister et al J Biol Chem. 2015 May 8;290(19):12058-67

Insert

Src homology 2 domain-containing phosphatase 2

Tags

GST

Expression

Bacterial

Mutation

Deleted amino acid residues 219-597, changed T73 …

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only
pSG5 N-HA SADBs shRNA resistant

Plasmid

#67157

Purpose

N-terminally HA tagged mouse SADBs containing substitutions providing resistance to an shRNA

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

Brsk1 (Brsk1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Nucleotide 294 C-T Nucleotide 297 C-G (shRNA resi…

Promoter

SV40 promoter

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pRS424-TUB2 - human TUBB1 Cterminal tail

Plasmid

#60400

Purpose

Expression of chimeric yeast beta tubulin (TUB2) - human beta6 tubulin Cterminal tail (TUBB1) using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

TUB2 amino acids 429 - end, replaced with human T…

Promoter

GAL

Available Since

May 6, 2015

Availability

Academic Institutions and Nonprofits only