We narrowed to 3,238 results for: zebrafish

1. pCS2-ER-EmEGFP

   Plasmid

   #169000

   Purpose

   Express ER-EmEGFP in zebrafish

   Depositor

   Noboru Mizushima

   Article

   Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

   Insert

   ER-EGFP (encoding the signal sequence of human calreticulin mRNA and EGFP-KDEL) (CALR Synthetic, Human)
   

   Use

   Unspecified

   Tags

   EGFP

   Available Since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT-ubb-cytoBirA-2a-mCherry

   Plasmid

   #79885

   Purpose

   Ubiquitin promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Tags

   3x HA

   Expression

   Bacterial

   Promoter

   Ubiquitin

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMT-MCS-cytoBirA-2A-mCherry_Ras

   Plasmid

   #80058

   Purpose

   MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Tags

   3x HA

   Expression

   Bacterial

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tol2-LyzC-GFP-U6ac-rac2-guides

   Plasmid

   #168241

   Purpose

   "neutrophil specific GFP with ubiquitous rac2 sgRNAs"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   rac2 sgRNAs
   

   Use

   CRISPR

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX

   Plasmid

   #75177

   Purpose

   Nuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   H2B-mCerulean3-P2A-mKOFP2-CAAX
   

   Use

   Three prime entry vector for multisite gateway th…

   Promoter

   n/a

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. actc1b-mKate2-EHD2b

   Plasmid

   #109517

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to EHD2b

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   EHD2b (ehd2 Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   mKate2

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tol2-LyzC-GFP-U6ac-cdk2-guides

   Plasmid

   #168250

   Purpose

   "neutrophil specific GFP with ubiquitous cdk2 sgRNAs"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   cdk2 sgRNAs
   

   Use

   CRISPR

   Available Since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-21a_2xNLS_FnCpf1_MBP_protein_expression

   Plasmid

   #120801

   Purpose

   Protein expression plasmid for 2xNLS FnCpf1 in pET-21a backbone

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   2xNLS_FnCpf1
   

   Use

   CRISPR

   Tags

   MBP and NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. FnoCas12a-DR-cr in PBCSK-

   Plasmid

   #126642

   Purpose

   Cloning vector FnoCas12a DR-crRNA for expression in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

   Insert

   FnoCas12a Full Length Direct Repeat crRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6 Promoter

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. actc1b-mKate2-ARF6

    Plasmid

    #109537

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to ARF6

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    ARF6 (arf6a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDestTol2CR-QUAS:NTR-mKate2

    Plasmid

    #241914

    Purpose

    Zebrafish transgenesis plasmid encoding nitroreductase-mKate2, driven by the QUAS enhancer, and including a cmlc2:mKate2 cardiac transgenesis marker

    Depositor

    Balázs Enyedi

    Article

    Fazekas et al J Cell Biol. 2026 Jan 5;225(1):e20250116

    Insert

    QUAS enhancer driving nitroreductase-mKate2
    

    Use

    Zebrafish tol2 transgenesis

    Tags

    nitroreductase fused with mKate2

    Promoter

    QUAS

    Available Since

    Oct. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAX1_HS∆P2

    Plasmid

    #225197

    Purpose

    Used to create a markerless deletion of the E. coli HS P2 phage

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    P2 phage homology arms
    

    Expression

    Bacterial

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAX1_HS∆tum

    Plasmid

    #225198

    Purpose

    Used to create a markerless deletion of the E. coli HS tum gene

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    tum homology arms
    

    Expression

    Bacterial

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAX1_HS_gpN:SpyTag

    Plasmid

    #225193

    Purpose

    Used for adding C-terminal SpyTag to the E. coli HS P2 phage major capsid protein

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    gpN homology arms and SpyTag
    

    Expression

    Bacterial

    Available Since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2+-FLAG3-hsN-drAgo2

    Plasmid

    #221717

    Purpose

    Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in zebrafish embryos. Swapped N-domain for human Ago2 N-domain.

    Depositor

    David Bartel

    Article

    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

    Insert

    hsN-drAgo2 (ago2 Zebrafish)
    

    Use

    Fish expression

    Tags

    3xFLAG

    Mutation

    Swap of N-domain for human Ago2 N-domain

    Promoter

    simian CMV IE94

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. ACVR1-TSAA

    Plasmid

    #206179

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations S192A and S194A

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Mutation

    S192A, S194A

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. ACVR1-TAAS

    Plasmid

    #206180

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations S190A and S192A

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Mutation

    S190A, S192A

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. ACVR1-TASA

    Plasmid

    #206181

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations S190A and S194A

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Mutation

    S190A, S194A

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCK005 ubi:mCerulean_ubb-polyA

    Plasmid

    #195979

    Purpose

    Expression construct

    Depositor

    Alexa Burger, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    ubi:mCerulean
    

    Use

    Other

    Available Since

    Feb. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcryGFP-acta1-dmdGFP

    Plasmid

    #172530

    Purpose

    alphaA-crystallin promoter (cry) drives GFP expression in the lens. The acta1 muscle-specific promoter drives expression of GFP-tagged zebrafish dmd.

    Depositor

    Joachim Berger

    Article

    Berger et al J Cell Mol Med. 2020 Jun;24(12):6680-668

    Insert

    dmd (dmd Zebrafish)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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