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pTECH-chPylRS(IPYE)

Plasmid

#99222

Purpose

Expression plasmid encoding chPylRS(IPYE) and pylT

Depositor

Dieter Söll

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Plpp chPyl(IPYE)RS; PproK pylT

Expression

Bacterial

Mutation

Contains activating mutations V31I, T56P, H62Y, A…

Promoter

Plpp and PproK

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pIVT-dCas9-EGFP

Plasmid

#174442

Purpose

in vitro transcription template for dCas9-EGFP

Depositor

Michael Lampson

Article

Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

Insert

Cas9 Endonuclease Dead

Use

In vitro transcription template

Tags

EGFP

Promoter

T7 SP6

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pmScarlet3-H_I3

Plasmid

#239343

Purpose

Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3-H in mammalian cells. Assembles into nanocages tagged with 60 FPs.

Depositor

Torsten Wittmann

Article

Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

Insert

I3-01

Tags

mScarlet3-H (a.k.a. mYongHong)

Expression

Mammalian

Mutation

K129A

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB402SN

Plasmid

#224551

Purpose

Empty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.

Depositor

Paul Schulze-Lefert

Article

Lawson et al bioRxiv 2024.07.14.603419

Type

Empty backbone

Tags

HA-Twin-Strep

Expression

Plant

Promoter

2 X 35S

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCasKP-hph

Plasmid

#117232

Purpose

Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_HDAC1

Plasmid

#183297

Purpose

All-in-One CRISPRko system with a guide RNA that targets HDAC1 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

HDAC1

Use

Lentiviral

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2 SV40 Cyclone-RLuc

Plasmid

#247494

Purpose

Acyclovir-regulated Renilla luciferase

Depositor

Samie Jaffrey

Article

Hou et al Nat Methods. 2025 Nov 3. doi: 10.1038/s4

Insert

Renilla luciferase gene with Cyclone insertion

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Mutation

Cyclone was inserted after Q288 of Renilla lucife…

Promoter

SV40

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTECH-MmPylRS(IPYE)

Plasmid

#104073

Purpose

Expression plasmid encoding MmPylRS(IPYE) and pylT

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Plpp MmPylRS(IPYE); PproK pyl

Expression

Bacterial

Mutation

Contains activating mutations V31I, T56P, H62Y, A…

Promoter

Plpp and PproK

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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GAL-SUV39H2-L

Plasmid

#89136

Purpose

mammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase linked to GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

Insert

SUV39H2 (SUV39H2 Human)

Tags

GAL4 DBD

Expression

Mammalian

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pBT13-splitD

Plasmid

#122597

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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