Addgene

We narrowed to 13,545 results for: NTS

Showing: 221 - 240 of 13545 results

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pSRK51

Plasmid

#223722

Purpose

Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

Depositor

Sreejith Raran-Kurussi

Article

Parida et al Curr Res Struct Biol. 2023 Sep 30;6:1001

Insert

TEV protease

Use

Tags

NT* (self cleaving)

Expression

Bacterial

Mutation

L56V/S135G/S219V

Promoter

T7

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pMC0x_gRNA_Pos5b_Ptet

Plasmid

#179338

Purpose

gRNA Position Vector 5b, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMC0x_gRNA_Pos5a_Ptet

Plasmid

#179337

Purpose

gRNA Position Vector 5a, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-5'UTR-MAVS-ORF1(KaganE49)

Plasmid

#52006

Purpose

Expresses the human MAVS CDS and contains the 5'UTR sequence with a mutation in the start codon 37 nt upstream of the FL MAVS start codon

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

5utr-MAVS-ORF1mut

Use

Tags

Expression

Mammalian

Mutation

mutation removing the start codon for ORF1

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-5'UTR-MAVS-ORF3,4(KaganE50)

Plasmid

#52007

Purpose

Expresses the human MAVS CDS and contains the 5'UTR sequence with a mutation in the start codons 61 and 67 nt downstream of the FL MAVS start codon

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

5utr-MAVS-ORF2,3mut

Use

Tags

Expression

Mammalian

Mutation

mutated start codons for ORF3 and 4

Promoter

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pJK637

Plasmid

#72437

Purpose

Acetobacter aceti 1023 mutS, partial gene

Depositor

T. J. Kappock

Insert

DNA mismatch repair protein MutS (partial gene)

Use

Tags

Expression

Bacterial

Mutation

deletes nt 1-767 and nt 2086-2655 of mutS gene

Promoter

n/a

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pST_116_LVL2 cam

Plasmid

#179332

Purpose

NT-CRISPR plasmid for a single gRNA.

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pST_119_LVL2 cam

Plasmid

#179333

Purpose

NT-CRISPR plasmid for integration of multiple gRNAs.

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-75-2

Plasmid

#73036

Purpose

Express an NT-3-BDNF hybrid neurotrophic factor, with reduced affinity to p75NTR, in mammalian cells

Depositor

Pantelis Tsoulfas

Article

Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

Insert

p75-2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Mature Human NT-3 gene exchanged Aspartic 15 to a…

Promoter

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pST_140_LVL2 cam

Plasmid

#179334

Purpose

NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout

Use

CRISPR

Tags

Expression

Mutation

Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…

Promoter

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pRAR064

Plasmid

#185031

Purpose

400 nt l31 promoter region, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Use

Tags

Expression

Bacterial

Mutation

Nucleotides of rpmE 5'UTR scrambled, Only fi…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR102

Plasmid

#185033

Purpose

400 nt l31 promoter region, l31 5'UTR U54C mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Use

Tags

Expression

Bacterial

Mutation

U54C mutation of rpmE 5'UTR, Only first 90 n…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR106

Plasmid

#185036

Purpose

400 nt l31 promoter region, l31 5'UTR G79U mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Use

Tags

Expression

Bacterial

Mutation

G79U mutation of rpmE 5'UTR, Only first 90 n…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR116

Plasmid

#185040

Purpose

400 nt l31 promoter region, l31 5'UTR U58C and U70C, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Use

Tags

Expression

Bacterial

Mutation

U58C and U70C of rpmE of 5'UTR, Only first 9…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR017-L31-sfGFP

Plasmid

#185024

Purpose

400 nt l31 promoter region, l31 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Use

Tags

Expression

Bacterial

Mutation

Only first 90 nt of rpmE coding present

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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CaV1.3e[8a,11,31b,Δ32,Δ42a] mut

Plasmid

#26577

Depositor

Diane Lipscombe

Article

Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

Insert

CACNA1D (Cacna1d Rat)

Use

Tags

Expression

Mammalian

Mutation

Contains exons 8a, 11, and 31b.Exons 32 and 42a a…

Promoter

Available since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pRAR104

Plasmid

#185034

Purpose

400 nt l31 promoter region, l31 5'UTR A74 and A76 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Use

Tags

Expression

Bacterial

Mutation

A74 and A76 deletion of rpmE 5'UTR, Only fir…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR105

Plasmid

#185035

Purpose

400 nt l31 promoter region, l31 5'UTR G52 and G79 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Use

Tags

Expression

Bacterial

Mutation

G52 and G79 deletion of rpmE 5'UTR, Only fir…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR107

Plasmid

#185037

Purpose

400 nt l31 promoter region, l31 5'UTR AGA74-76GAG mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Use

Tags

Expression

Bacterial

Mutation

AGA74-76GAG mutation of rpmE 5'UTR, Only fir…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR109

Plasmid

#185038

Purpose

400 nt l31 promoter region, l31 5'UTR G60 and C69 deletion, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Use

Tags

Expression

Bacterial

Mutation

G60 and C69 deletion of rpmE 5'UTR, Only fir…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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