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pGEX4T3-NL2CT
Plasmid#163623Purposemouse NL2 N-terminally tagged with GSTDepositorInsertmouse NL2 (Nlgn2 Mouse)
UseTagsGSTExpressionMammalianMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST-eGFP-C1-VPS35
Plasmid#163622Purposehuman VPS35 cDNA C-terminally tagged with eGFPDepositorInserthuman VPS35 (VPS35 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMito-mCherry-FRB
Plasmid#59352PurposeExpresses mCherry-tagged MitoTrap in mammalian cells.DepositorTypeEmpty backboneUseTagsFRB and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mSNX27
Plasmid#163617Purposemouse SNX27 cDNA N-terminally tagged with GFPDepositorInsertmouse SNX27 (Snx27 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMycoFos
Plasmid#84577PurposeShuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium. Copy number is controllable by arabinose in E.coli.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 7, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
AgAK
Plasmid#71290PurposeExpression of A. gambiae adenosine kinaseDepositorInsertAdenosine Kinase
UseTags6X HistidineExpressionBacterialMutationPromoterT7Available sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHB1
Plasmid#204668PurposepUC19 containing the LL-FRT-erm-FRT-spacer sequence which serves as template for BarSeq deletion constructsDepositorInsertFRT-erm-FRT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-Atg32-deltaPsR pXP721
Plasmid#200735PurposePlasmid for the expression of GFP-Atg32 lacking the PsR domain in S. cerevisiae.DepositorInsertAtg32
UseTagsGFPExpressionYeastMutationAmino acids 200-341 are deleted.PromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
Atg20-24 pST39
Plasmid#200439PurposeHis6 tagged Atg20 and untagged Atg24 from S. cerevisiae for co-expression in E. coli.DepositorInsertsAtg20
Atg24
UseTags6xHis-TEVExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVER-LacI
Plasmid#164830PurposeLacI protein regulates expression of yellow fluorescent protein. For measuring dose-response curves.DepositorInsertLacI
UseSynthetic BiologyTagsExpressionMutationPromoterPtacAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTY1-LacI
Plasmid#164831PurposeLacI protein regulates the expression of a tetracycline resistance gene and yellow fluorescent proteinDepositorInsertLacI
UseSynthetic BiologyTagsExpressionMutationPromoterPtacAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-Myo19
Plasmid#127612PurposeGFP-tagged Myosin-19DepositorInsertMyo19
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiCas9-Blast
Plasmid#52962PurposeExpresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.DepositorHas ServiceLentiviral PrepInsertsCas9
Blasticidin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS-NSAvailable sinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSerOTS-C1* (V70)
Plasmid#188537PurposePhosphoserine incorporation into recombinant proteinsDepositorInsertspSerRS
Ef-pSer
tRNApSer
UseTagsExpressionBacterialMutationmutations resulting from laboratory evolutionPromoterglnS*Available sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV643
Plasmid#226182PurposeExpresses a color marker (uidA) cassette for disruption on KU70 gene in K. phaffii.DepositorInsertuidA marker flanked with targeting regions to KU70 gene in K.phaffii
UseTagsExpressionBacterialMutationPromoterTEF1pAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide Cherry
Plasmid#170510PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1a and hU6Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1-tTA/Pur
Plasmid#171683Purposeconstitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (puromycin)DepositorInsertstetracycline-controlled transactivator
Puromycin
EF-1a promoter
UseTagsExpressionMammalianMutationPromoterEF-1a promoter and RPBSA promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Atg24 1B
Plasmid#197046PurposeHis6 tagged Atg24 from S. cerevisiae for expression in E. coli.DepositorInsertAtg24
UseTags6His-TEVExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pMito-PAGFP-FRB.
Plasmid#60005PurposeExpresses MitoTrap tagged with photo-activatable GFP in mammalian cells.DepositorTypeEmpty backboneUseKnocksidewaysTagsFRB and PAGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mSNX27-ΔF3
Plasmid#163618Purposemouse SNX27 without the F3 domain cDNA C-terminally tagged with GFPDepositorInsertmouse SNX27-ΔF3 (Snx27 Mouse)
UseTagsGFPExpressionMammalianMutationdeletion F3 domainPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mSNX27-H112A
Plasmid#163619Purposemouse SNX27 cDNA with a point mutation H112A C-terminally tagged with GFPDepositorInsertmouse SNX27-H112A (Snx27 Mouse)
UseTagsGFPExpressionMammalianMutationH112APromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gfap-Cryab
Plasmid#58947Purposehamster Cryab gene under the control of the human GFAP promoter to target expression to astrocytesDepositorInsertCryab
UseMouse Targeting; Transgene expressionTagsExpressionMammalianMutationgenomic fragmentPromoterhuman GFAP promoter (position -2163 to +47)Available sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
Atg32 [200-341] 1B
Plasmid#190431PurposeRecombinant expression plasmid of S. cerevisiae Atg32 amino acids 200 - 341 in E. coli.DepositorInsertAtg32
UseTags6xHis-TEVExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCas9n(D10A)-Blast
Plasmid#63593PurposeExpresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.DepositorInsertsCas9
Blasticidin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationD10A, nickase mutantPromoterEFS-NSAvailable sinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mSNX27 RNAi-resistant
Plasmid#163620Purposemouse SNX27 cDNA with point mutation to resist siRNA against SNX27 C-terminally tagged with GFPDepositorInsertmouse SNX27 RNAi-resistant (Snx27 Mouse)
UseTagsGFPExpressionMammalianMutationRNAi resistancePromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCF226
Plasmid#121667PurposeEFS-Cas9-wt-P2A-PuroDepositorInsertcas9
UseLentiviralTagsExpressionMutationPromoterEF-1a core promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDIV272
Plasmid#226183PurposeExpresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.DepositorInserttRNA-sgRNA-tRNA
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterTEF1p (Komagataella phaffii)Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDIV151
Plasmid#226180PurposeExpresses Cas9 in a backbone compatible with K. phaffii.DepositorInsertCas9
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterGAP1Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEFL
Plasmid#38121DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEF-1Available sinceSept. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-AP-Smo-YFP-DEST
Plasmid#49099PurposeExpressing AP-Smo-YFP in mammalian cells, can be used for establishing Flp-In stable cell linesDepositorInsertSmo (Smo Mouse)
UseTagsYFP and acceptor peptide (AP)ExpressionMammalianMutationPromoterT7Available sinceNov. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
SpRY
Plasmid#198544PurposeExpresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSDDepositorInsertSpRY
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV CAGG eGFP
Plasmid#107707Purposeexpress eGFP in variety of cell typesDepositorInserteGFP
UseAAVTagsExpressionMammalianMutationPromoterCAGGAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5RS-HERCD533
Plasmid#65224PurposeExpression of dominant negative EGFR in mammalian cellsDepositorInsertHERCD533 (EGFR Human)
UseTagsExpressionMammalianMutationdeleted AA 654-1186PromoterCMVAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDIV270
Plasmid#226181PurposeExpresses Cas9 and gRNA targeting KU70 gene in K. phaffii.DepositorInserttRNA-sgRNA-tRNA
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterTEF1p (Komagataella phaffii)Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
ALFY-FYVE 1G
Plasmid#197045PurposeExpression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.DepositorInsertALFY (WDFY3 Human)
UseTags6xHis-GST-TEVExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-Squ:mCherry
Plasmid#20163DepositorInsertspaghetti squash (squ, myosin II RLC) 5' UTR and ORF fused to mCherry followed by squ 3' UTR (sqh Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
HGXPRT
Plasmid#64201PurposeExpression of Plasmodium falciparum HGXPRTDepositorInsertHypoxanthine guanine xanthine phosphoribosyltransferase
UseTagsHistidine tagExpressionBacterialMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only
