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Plasmid#174796PurposeBacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)DepositorInsertPARP1-A774L (PARP1 Human)
UseTags6xHisExpressionBacterialMutationA774L, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-W318R
Plasmid#174793PurposeBacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)DepositorInsertPARP1-W318R (PARP1 Human)
UseTags6xHisExpressionBacterialMutationW318, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-A762V
Plasmid#174795PurposeBacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)DepositorInsertPARP1-A762V (PARP1 Human)
UseTags6xHisExpressionBacterialMutationA762VPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41a-MED22
Plasmid#15432DepositorInsertMED22 (MED22 Human)
UseTagsGST and HisExpressionBacterialMutationN/APromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET32b-MED19
Plasmid#15438DepositorInsertMED19 (Med19 Mouse)
UseTagsHis and TrxExpressionBacterialMutationPromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET41a-MED30
Plasmid#15431DepositorInsertMED30 (Med30 Mouse)
UseTagsGST and HisExpressionBacterialMutationN/APromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET41a-MED31
Plasmid#15437DepositorInsertMED31 (Med31 Mouse)
UseTagsGST and HisExpressionBacterialMutationN/APromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
His-FLAG-Med18 pBacPAK8
Plasmid#15371DepositorInsertMed18 (MED18 Human)
UseBaculovirus constructionTagsFLAG and HisExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Med11 pBacPAK8
Plasmid#15373DepositorInsertMed11 (Med11 Mouse)
UseBaculovirus constructionTagsFLAGExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
myc-Med22 pBacPAK8
Plasmid#15374DepositorInsertMed22 (MED22 Human)
UseBaculovirus constructionTagsmycExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
myc-Med31 pBacPAK8
Plasmid#15375DepositorInsertMed31 (Med31 Mouse)
UseBaculovirus constructionTagsmycExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
myc-Med19 pBacPAK8
Plasmid#15376DepositorInsertMed19 (Med19 Mouse)
UseBaculovirus constructionTagsmycExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-DXO
Plasmid#177993PurposeFor purification of N-terminally His-tagged DXO from bacteriaDepositorInsertDXO (DXO Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn1-L713F
Plasmid#173937PurposeBacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutationDepositorInsertPARP1delZn1-L713F (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 1-96; L713F, V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn3-L713F
Plasmid#173934PurposeBacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutationDepositorInsertPARP1delZn3-L713F (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 214-373; L713F, V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-G871L
Plasmid#174798PurposeBacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)DepositorInsertPARP1-G871L (PARP1 Human)
UseTags6xHisExpressionBacterialMutationG871L, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-A870L
Plasmid#174797PurposeBacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)DepositorInsertPARP1-A870L (PARP1 Human)
UseTags6xHisExpressionBacterialMutationA870L, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-K893I
Plasmid#174799PurposeBacterial expression of an inactive mutant (K893I may disrupt NAD+ binding)DepositorInsertPARP1-K893I (PARP1 Human)
UseTags6xHisExpressionBacterialMutationK893I, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41sIII_GST-ALC1-K77R
Plasmid#174813PurposeBacterial expression of inactive ALC1 (K77R disrupts ATP binding)DepositorInsertALC1-K77R (CHD1L Human)
UseTagsGSTExpressionBacterialMutationK77RPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41sIII_GST-ALC1-E175Q
Plasmid#174814PurposeBacterial expression of inactive ALC1 (E175Q disrupts ATP binding)DepositorInsertALC1-E175Q (CHD1L Human)
UseTagsGSTExpressionBacterialMutationE175QPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MED20
Plasmid#15435DepositorInsertMED20 (Med20 Mouse)
UseTagsHisExpressionBacterialMutationN/APromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-28a (+)-hTBP
Plasmid#171073PurposeExpresses human TBP in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TBPDepositorInsertTATA-box-binding protein (TBP Human)
UseTagsHisExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Flag-KIAA0947
Plasmid#49248PurposeMammalian expression of flag-tagged human KIAA0947DepositorInsertKIAA0947 (ICE1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Flag-Med26
Plasmid#49250PurposeMammalian expression of flag-tagged Med26DepositorInsertMed 26 (MED26 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MED19
Plasmid#15420DepositorInsertMED19 (Med19 Mouse)
UseTagsFLAGExpressionMammalianMutationN/APromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnk9-IRES-EGFP
Plasmid#164949PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnk9 and EGFPDepositorInsertkcnk9 (kcnk9 Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationPromoteractinb (actb2) promoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MED10
Plasmid#15417DepositorInsertMED10 (Med10 Mouse)
UseTagsFLAGExpressionMammalianMutationN/APromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
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FLAG-MED27
Plasmid#15424DepositorInsertMED27 (MED27 Human)
UseTagsFLAGExpressionMammalianMutationN/APromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MED21
Plasmid#15422DepositorInsertMED21 (MED21 Human)
UseTagsFLAGExpressionMammalianMutationN/APromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MED11
Plasmid#15418DepositorInsertMED11 (Med11 Mouse)
UseTagsFLAGExpressionMammalianMutationN/APromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MED30
Plasmid#15426DepositorInsertMED30 (Med30 Mouse)
UseTagsFLAGExpressionMammalianMutationN/APromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj13-EGFP
Plasmid#164940PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 fused with EGFP at the C terminusDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsEGFPExpressionMutationStop codon deletedPromoteractinb (actb2) promoterAvailable sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MED20
Plasmid#15421DepositorInsertMED20 (Med20 Mouse)
UseTagsFLAGExpressionMammalianMutationN/APromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MED31
Plasmid#15427DepositorInsertMED31 (Med31 Mouse)
UseTagsFLAGExpressionMammalianMutationN/APromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-pax3a-kcnj13-IRES-EGFP
Plasmid#164950PurposeTol2 construct: pax3a-5.4k promoter driven zebrafish kcnj13 fused with EGFP and EGFPDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationPromoterpax3a promoter -5.4kAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj13-IRES-EGFP
Plasmid#164941PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 and EGFPDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationPromoteractinb (actb2) promoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
His-FLAG-Med20 pBacPAK8
Plasmid#15372DepositorInsertMed20 (Med20 Mouse)
UseBaculovirus constructionTagsFLAG and HisExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-GAL4-PARP1-CAT-L713F
Plasmid#175949PurposeBacterial expression of a fusion of yeast GAL4 DNA binding domain and human PARP1 CAT domain containing L713F gain-of-function mutationDepositorInsertGAL4 DNA binding domain (GAL4 Budding Yeast)
UseTags6xHis tagExpressionBacterialMutationL713F, V762A for the 'PARP1-CAT-L713F' …PromoterT7Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj1b-IRES-EGFP
Plasmid#164946PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnj1b and EGFPDepositorInsertkcnj1b (kcnj1b Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPExpressionMutationPromoteractinb (actb2) promoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only