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We narrowed to 1,219 results for: cas12

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Showing: 441 - 480 of 1219 results

  1. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028)

    Plasmid
    #114084
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.4)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimi…
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114084-plasmid-map-sequence-id-230536

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  2. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-ecDHFR-enOsCas12f1-NLS-ecDHFR_pA_AAV2 ITR

    Plasmid
    #197029
    Purpose
    AAV vector encoding a human codon-optimized ecDHFR-enOsCas12f1-ecDHFR driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6
    Depositor
    Xiangfeng Kong
    Article
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Insert
    humanized ecDHFR-enOsCas12f1-ecDHFR
    Use
    AAV
    Mutation
    D52R / T132R
    Promoter
    MHCK7, hU6
    Available Since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197029-plasmid-map-sequence-id-388970

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  3. pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.1) (RTW1352)

    Plasmid
    #114081
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.1
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.1)
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimi…
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114081-plasmid-map-sequence-id-231038

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  4. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)

    Plasmid
    #114082
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimi…
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114082-plasmid-map-sequence-id-220504

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  5. pUC19-SARS-CoV-2-N-gene-truncated

    Plasmid
    #240635
    Purpose
    pUC19-SARS-CoV-2-N-gene-truncated plasmid is a plasmid that contains the SARS-CoV-2 N-gene and can be used as a positive control in SARS-CoV-2 research or diagnostics assays targeting the N gene.
    Depositor
    Kevin Yehl
    Insert
    SARS-CoV-2-N-gene-truncated (N )
    Use
    Synthetic Biology
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    240635-plasmid-map-sequence-id-486094

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  6. pKb-TnpB2

    Plasmid
    #215334
    Purpose
    ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site
    Depositor
    Kutubuddin Molla
    Article
    Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10
    Insert
    Rice codon optimized ISDra2 TnpB
    Tags
    Not applicable
    Expression
    Plant
    Promoter
    Rice ubiquitin promoter for TnpB and maize ubiqui…
    Available Since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    215334-plasmid-map-sequence-id-447325

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  7. pKb-TnpB1

    Plasmid
    #215333
    Purpose
    ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site
    Depositor
    Kutubuddin Molla
    Article
    Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10
    Insert
    Rice codon optimized ISDra2 TnpB
    Tags
    Not applicable
    Expression
    Plant
    Promoter
    Rice ubiquitin promoter for TnpB and OsU3 promote…
    Available Since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    215333-plasmid-map-sequence-id-446611

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  18. AcrVA1_cpGR2(GGS5)-S103ΔELS_(GGS)2-(GGS)2

    Plasmid
    #246051
    Purpose
    Expression of AcrVA1-cpGR2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS and GGS2 linkers; enables chemogenetic control of MbCas12a
    Depositor
    Dominik Niopek
    Article
    Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk
    Insert
    AcrVA1_cpGR2-S103ΔELS
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Mutation
    ΔELS
    Available Since
    Dec. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    246051-plasmid-map-sequence-id-495343

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  19. H1_sgRNA(GRIN2B)_SauCas9_AcrIIA5-AsLOV2(N77)

    Plasmid
    #246056
    Purpose
    Expression of a sgRNA targeting the GRIN2B locus, a SauCas9 and an AcrIIA5-cpGR2 hybrid (insertion before N77) linked through a P2A sequence, under the same pol-III H1 promoter
    Depositor
    Dominik Niopek
    Article
    Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk
    Insert
    SauCas9, sgRNA(GRIN2B) and AcrIIA5_AsLOV2-N77
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    246056-plasmid-map-sequence-id-495342

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  20. H1_sgRNA(GRIN2B)_SauCas9_AcrIIA5-AsLOV2(D41)

    Plasmid
    #246055
    Purpose
    Expression of a sgRNA targeting the GRIN2B locus, a SauCas9 and an AcrIIA5-cpGR2 hybrid (insertion before D41) linked through a P2A sequence, under the same pol-III H1 promoter
    Depositor
    Dominik Niopek
    Article
    Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk
    Insert
    SauCas9, sgRNA(GRIN2B) and AcrIIA5_AsLOV2-D41
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Dec. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    246055-plasmid-map-sequence-id-495341

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  35. pYPQ301

    Plasmid
    #205415
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Hs1Cas12a without promoter
    Depositor
    Yiping Qi
    Article
    Li et al Front Genome Ed. 2023 Oct 12;5:1251903.
    Insert
    Hs1Cas12a
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Expression
    Plant
    Available Since
    Nov. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    205415-plasmid-map-sequence-id-414100

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  36. pYPQ300

    Plasmid
    #205414
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Ev1Cas12a without promoter
    Depositor
    Yiping Qi
    Article
    Li et al Front Genome Ed. 2023 Oct 12;5:1251903.
    Insert
    Ev1Cas12a
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS (N terminal on insert) NLS (C terminal on ins…
    Expression
    Plant
    Available Since
    Nov. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    205414-plasmid-map-sequence-id-414101

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  38. pTE2690

    Plasmid
    #137717
    Purpose
    Expresses dimpLbCas12a-SunTag in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576
    Insert
    dimpLbCas12a
    Use
    CRISPR
    Tags
    NLSx3 SV40 and SunTagx10
    Expression
    Mammalian
    Mutation
    D156R, G532R, K538V, Y542R, K595R, D832A,
    Promoter
    CMV
    Available Since
    March 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    137717-plasmid-map-sequence-id-274029

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Showing: 441 - 480 of 1219 results