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pCAG-Ace1_Q89D_D100N_E206V-HaloTag-ST

Plasmid

#129260

Purpose

Express soma-localized Ace1_Q89D_D100N_E206V-HaloTag in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Ace1_Q89D_D100N_E206V-HaloTag-ST

Use

Tags

Expression

Mammalian

Mutation

Q89D, D100N, E206V

Promoter

CAG

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pIAGO-pegA + aceS

Plasmid

#114457

Purpose

Expression of PegA and AceS in streptomycetes

Depositor

Patrick Caffrey

Article

Sheehan et al Mol Biosyst. 2017 May 2;13(5):866-873. d

Insert

pegA + aceS

Use

Tags

Expression

Bacterial

Mutation

Promoter

ermE

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-spikeRBD::∆ACE2 SURF

Plasmid

#206957

Purpose

Fluorescent reporter of the interaction between SARS-CoV-2 spike and human ACE2

Depositor

Xiaokun Shu

Article

Yang et al Nat Microbiol. 2023 Jan;8(1):121-134. do

Inserts

sars GU280_gp02, spike glycoprotein (S SARS-CoV-2)
ACE2 (ACE2 Human)

Use

Tags

cSURF, mCherry, and nSURF

Expression

Mammalian

Mutation

17-615 and RBD (319-541)

Promoter

CMV

Available since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-pAce-Kv2.1PR

Plasmid

#195529

Purpose

Green fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAce-Kv2.1 proximal restriction sequence

Use

AAV

Tags

Expression

Mammalian

Mutation

Ace-mNeon 78K, 81D, 92N, 178F; SY linker

Promoter

CaMKII

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-His6-GFP-C-LACE

Plasmid

#133913

Purpose

Bacterial expression of His6-GFP-C-LACE

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

superfolder green fluorescent protein

Use

Tags

LACE tag (GSGPRKVIKMESEE) and TEV-cleavable His6-…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PARP2-Flag-His-WT

Plasmid

#111574

Purpose

Expresses full length PARP2 in Sf9 (insect) cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP2 (PARP2 Human)

Use

Tags

C-terminal 6xHis and C-terminal Flag

Expression

Insect

Mutation

Promoter

polyhedrin promoter

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-Ace-mNeon2

Plasmid

#195518

Purpose

Green fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

Ace-mNeon2

Use

AAV

Tags

Expression

Mammalian

Mutation

Ace-mNeon 81S; SY linker

Promoter

Synapsin

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-surfaceHA-IRES-Puro

Plasmid

#207321

Purpose

Lentiviral expression of synthetic HA surface receptor in Mammalian cells

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

HAtag-Myctag-PDGFRB-TM-IRES-Puro

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha_short (EFS)

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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hBACE1(D289N).ires.mCherry-pcDNA3

Plasmid

#204363

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

BACE1 (BACE1 Human)

Use

Tags

Expression

Mammalian

Mutation

D289N

Promoter

CMV/T7 RNApol

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-Ace-mNeon2

Plasmid

#195526

Purpose

Green fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

Ace-mNeon2

Use

AAV

Tags

Expression

Mammalian

Mutation

Ace-mNeon 81S; SY linker

Promoter

CaMKII

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pExp-xMBP-ACE2-CHis

Plasmid

#194998

Purpose

Production of ACE2 extracellular domain

Depositor

Marko Hyvönen

Article

Harman et al J Med Chem. 2023 Jul 27;66(14):9881-9893

Insert

ACE2 (ACE2 Human)

Use

Tags

His8 and xMBP

Expression

Bacterial

Mutation

Promoter

T7lac

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2-WT(732)-IgG1

Plasmid

#154104

Purpose

Mammalian expression plasmid for dimeric soluble ACE2 (IgG1-Fc fusion)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease and neck domains …

Promoter

CMV

Available since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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Notch3 intracellular domain-pcw107-V5

Plasmid

#64623

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH3 (NOTCH3 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

intracellular domain *

Promoter

PGK

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pACEBac1-PARP1-Flag-His-WT

Plasmid

#111572

Purpose

Expresses full length PARP1 in Sf9 (insect) cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP1 (PARP1 Human)

Use

Tags

C-terminal 6xHis and C-terminal Flag

Expression

Insect

Mutation

Promoter

polyhedrin promoter

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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LV-Camk2-Ace-7aa-mScarlet

Plasmid

#129701

Purpose

Expresses Ace-7aa-mScarlet in mammalian cells

Depositor

Yiyang Gong

Article

Beck et al Sci Rep. 2019 Nov 4;9(1):15878. doi: 10.

Insert

Ace-7aa-mScarlet

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Camk2

Available since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pWPI-IRES-Puro-Ak-ACE2

Plasmid

#154985

Purpose

Lentiviral vector expressing Human ACE2

Depositor

Sonja Best

Insert

ACE2 (ACE2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-pAce-Kv2.1PR

Plasmid

#195523

Purpose

Green fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAce-Kv2.1 proximal restriction sequence

Use

AAV

Tags

Expression

Mammalian

Mutation

Ace-mNeon 78K, 81D, 92N, 178F; SY linker

Promoter

Synapsin

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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Notch1 intracellular domain-pcw107-V5

Plasmid

#64622

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH1 (NOTCH1 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

intracellular domain *

Promoter

PGK

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
AAV-hACE2-cMYC-Flag

Plasmid

#158957

Purpose

Used for expression of human ACE2 with AAV packaging

Depositor

Akiko Iwasaki

Insert

Human ACE2 (ACE2 Human)

Use

Tags

Flag and MYC

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2v2.4(732)-IgG1

Plasmid

#154106

Purpose

Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (IgG1-Fc fusion)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease and neck domains …

Promoter

CMV

Available since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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