Addgene

Showing: 221 - 240 of 480 results

Results per page:

Sort results by:

pGEX6P1-mCherry-Nanobody (LaM-2)

Plasmid

#162276

Purpose

Expresses GST-tagged anti-mCherry Nanobody (LaM-2) in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

Insert

mCherry-Nanobody (LaM-2)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW57.1-DUX4-CA

Plasmid

#99281

Purpose

All-in-one lentivector with tet-inducible DUX4 (codon sequences altered)

Depositor

Stephen Tapscott

Article

Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

Insert

DUX4 (codon altered) (DUX4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

codon altered

Promoter

TRE

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-SPORT6-RNF114-RINGmut

Plasmid

#69922

Purpose

Expresses mutant myc-RNF114 in mammalian cells

Depositor

Francesca Capon

Article

Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

Insert

RNF114 (RNF114 Human)

Use

Tags

Expression

Mammalian

Mutation

C29A, C32A

Promoter

CMV

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-SPORT6-RNF114-UIMmut

Plasmid

#69923

Purpose

Expresses mutant myc-RNF114 in mammalian cells

Depositor

Francesca Capon

Article

Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

Insert

RNF114 (RNF114 Human)

Use

Tags

Expression

Mammalian

Mutation

S224P

Promoter

CMV

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-Tag 3B/WT myc-M87

Plasmid

#87722

Purpose

Expresses WT myc-M87 spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

M87 isoform (deletion of nt 1-490 - 5'UTR an…

Promoter

CMV

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AV4-EF1-GFP

Plasmid

#124971

Purpose

Control for AV4-EF1-NMNAT2

Depositor

Kazuhiro Oka

Article

Ljungberg et al Hum Mol Genet. 2012 Jan 15;21(2):251-67.

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

EF1-alpha

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB(EXPR-PYL-DNMT1-NEO)

Plasmid

#114396

Purpose

For PYL-DNMT1 expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-DNMT1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUAST ɑSynWT-EGFP

Plasmid

#201249

Purpose

allows the expression of the insert in Drosophila melanogaster

Depositor

Pedro Domingos

Article

Pocas et al Hum Mol Genet. 2015 Apr 1;24(7):1898-907

Insert

ɑ-Synuclein (SNCA Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mTln1-F2-pET151

Plasmid

#177870

Purpose

Expresses mouse Talin 1 F2 domain in bacterial cells

Depositor

Ben Goult

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

mouse Talin 1 F2 domain (Tln1 Mouse)

Use

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mTln1-F2F3-pET151

Plasmid

#177877

Purpose

Expresses mouse Talin 1 F2F3 domains in bacterial cells

Depositor

Ben Goult

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

mouse Talin 1 F2F3 domain (Tln1 Mouse)

Use

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLM494

Plasmid

#100539

Purpose

CEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.

Depositor

Bernd Müller-Röber

Article

Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:

Inserts

GGPP synthase
phytoene desaturase
phytoene-beta carotene synthase
HMG CoA reductase

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

PGK1, TDH3, TIP1, and ZEO1

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES2Cx32WTEGFP

Plasmid

#78150

Purpose

Mammalian Expression of Connexin 32 and EGFP from a bicistronic mRNA

Depositor

Mario Bortolozzi

Article

Carrer et al Hum Mol Genet. 2017 Oct 25. pii: 4565496

Insert

Connexin 32 (GJB1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AV4-EF1-NMNAT2

Plasmid

#124970

Purpose

Express NMNAT2

Depositor

Kazuhiro Oka

Article

Ljungberg et al Hum Mol Genet. 2012 Jan 15;21(2):251-67.

Insert

NMNAT2, GFP (NMNAT2 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

EF1-alpha

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Frt-hspB10

Plasmid

#63101

Purpose

Expression of HSPB10 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb10 (ODF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IP-hWIPI2B-3×FLAG

Plasmid

#215510

Purpose

Expresses FLAG tagged human WIPI2B.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

WD-repeat protein interacting with phosphoinositides 2B (WIPI2 Human)

Use

Retroviral

Tags

3×FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB-HDAC5(mut-H-Y)

Plasmid

#114398

Purpose

For PYL-HDAC5 H1006Y expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC5 H1006Y

Use

Tags

Expression

Mammalian

Mutation

additional D593E compared to NCBI ref NP_005465.2

Promoter

CMV

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB(EXPR-PYL-HDAC5-NEO)

Plasmid

#114395

Purpose

For PYL-HDAC5 expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC5

Use

Tags

3xFlag-NLS (internal)

Expression

Mammalian

Mutation

D593E compared with NCBI reference NP_005465.2

Promoter

CMV

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB-HDAC5(mut-H-A)

Plasmid

#114397

Purpose

For PYL-HDAC5 H1006A expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC5 H1006A

Use

Tags

3xFlag-NLS (internal)

Expression

Mammalian

Mutation

D593E compared to NCBI ref NP_005465.2

Promoter

CMV

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB-EXPR(PYL-cat_dom_HDAC5)

Plasmid

#114401

Purpose

For PYL-HDAC5 catalytic domain expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC5 catalytic domain

Use

Tags

3xFlag-NLS (internal)

Expression

Mammalian

Mutation

S220F and the deletion of M221

Promoter

CMV

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB-EXPR(PYL-Nterm_dom_HDAC5)

Plasmid

#114402

Purpose

For PYL-HDAC5 N terminal domain expression

Depositor

Vincent Dion

Article

Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

Insert

PYL-HDAC5 N terminal domain

Use

Tags

3xFlag-NLS (internal)

Expression

Mammalian

Mutation

D593E compared to NCBI ref NP_005465.2

Promoter

CMV

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge